New bioprocessing applications enable manufacturing facilities built on the principle of flexibility, and focused on process and cost economics.
DRIVING BIOPROCESSING INDUSTRIALIZATION
N-1 perfusion refers to the use of an ATF device to achieve high cell density in the seed train step before the production bioreactor (N), achieving a more rapid and robust process while maintaining an existing Fed-batch based production scheme.
High density (HD) cell banking is seed train optimization involving larger volume frozen seed banks, eliminating seed steps from vial and enabling banking of larger volumes up to 1 liter.
A continuous capture process is the next big challenge for modern bioproduction. Multi-column chromatography operates in continuous mode, balancing downstream productivity with upstream titers.
Tangential flow filtration (TFF) is commonly used for concentration and diafiltration operations in a purification process. Concentration is the unit operation where the feed volume is reduced and the species retained by the membrane increases in relative concentration. Diafiltration is the convective elimination of permeable solutes passing to the permeate and the addition of fresh solvent to the retentate.