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OPUS® MiniChrom Columns are designed for bench-scale resin screening, process development and sample preparation.

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Internal diameter x Bed Height (cm)

High reproducibility

Superimposition of 25 column qualification curves

 

Designed for bench-scale resin screening, process development,  and sample preparation, OPUS® MiniChrom Columns are pre-packed with user-specified resin and supplied ready to use with any liquid chromatography system.  Flexible column dimension configurations (internal diameters, bed heights, volumes) allow comparative evaluation of process parameters and the construction of customized resin libraries.

Each OPUS® MiniChrom Column is validated and documented for column HETP and asymmetry. Individual laser labeling containing Part Number and Serial Number ensure traceability to the corresponding performance data. 

  • Pre-packed with user-specified chromatography resin
  • Packed with correct compression factor to deliver high performance from process resins
  • Compatible with all common liquid chromatography systems

 OPUS® MiniChrom Columns are available in the following formats:

Internal Diameter   Bed Height         Column Volume 
cm mm   cm   mm   mL  
               
0.5 5.0   1.0 10.0   0.2  
0.5 5.0   2.5 25.0   0.5  
0.5 5.0   5.0 50.0   1.0  
0.8 8.0   2.0 20.0   1.0  
0.8 8.0   5.0 50.0   2.5  
0.8 8.0   10.0 100.0   5.0  
1.13 11.3   5.0 50.0   5.0  
1.13 11.3   10.0 100.0   10.0  

 

A Certificate of Analysis (CoA) for each individual column is available on request.

  • Convenient, pre-packed columns provided ready to use
  • Flexible, packed with any user-specified process resin
  • Variable configurations of internal diameter, bed height, and column volume enable customization to specific process development needs
  • Compatible with any liquid chromatiography system
  • High reproducibility, ensures reliable performance from process resins batch to batch
  • Broad scalability, up to 10 ml volume and 3 diameters
  • Proven, technology currently platformed in various process development projects, as well as for resin sampling by various resin vendors
  • Certified, with individual column performance certificates available upon request
  • Cost-effective, small configuration drives reduced material needs, and saves valuable resin samples while delivering similar performance to larger preparative or process columns

Process development / Resin screening

The development of a purification workflow for a specific molecule requires evaluation of the resin and the operating conditions for each chromatography step.

Using a scale-down chromatography column configuration drives efficiency and higher throughput. OPUS® MiniChrom Columns perform equivalent to larger columns and yield reliable and predictive chromatography performance data. Using these smaller scaled-down columns for process development saves valuable samples, cost, resources and time.

Results can be saved in resin library of preferred resins.

Sample preparation

The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates.  Non-purified samples require sample preparation before analysis can be executed. OPUS® MiniChrom Columns enable one-step purification of multiple samples in parallel.

Use of OPUS® MiniChrom Columns generates 8-400 mg of sample per MiniChrom per run.

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system.  We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

LEARN MORE ABOUT REPLIGEN MANUFACTURING

Column inner diameter

5 mm

   

8 mm

    11.3 mm  

Packed bed height

10 mm

25 mm

50 mm

20 mm

50 mm

100 mm

50 mm

100 mm

Column volume

0.2 mL

0.5 mL

1.0 mL

1.0 mL

2.5 mL

5.0 mL

5.0 mL

10.0 mL

Inner cross-sectional area

0.2 cm2

   

0.5 cm2

   

1.0 cm2

 

Connectors

Fingertight 1/16", female (10-32)

Column material

Polypropylene

Chemical stability

All commonly used aqueous buffers, pH 1 – 14, organic solvents*.
Avoid the use of halogenated organic solvents, hexane

Column storage solution

  • AIEC and CIEC Columns: 20 % ethanol + 150 mM sodium chloride
  • HIC, SEC, Affinity and MMC Columns: 20 % ethanol
  • CHT, CFT Columns (Bio-Rad): 20 % ethanol + 10 mM sodium phosphate, pH 6.8
  • ProSep® Columns(Millipore):1 % benzyl alcohol + 0.1 M sodium acetate, pH 5.1

Recommended storage temperature

  • AIEC, CIEC, HIC, SEC and MMC: +4°C to +30°C
  • Affinity Columns: +2°C to +8°C

Working temperature

+4°C to +30°C

Maximum working pressure

Up to 30 bar

Flow velocity

16 – 1000 cm/h**

Labelling

Laser-etched on every individual column body. Label contains:

  • Product number
  • Serial number
  • Resin name
  • Column dimensions (ID x H, V)
  • Flow direction

* The chemical stability of the packed chromatography resin also has also to be taken into consideration. For more information, please refer to the manufacture’s resin specification.
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