RoboColumn.jpgOPUS® RoboColumns® are miniaturized pre-packed columns on a 96-well plate, for automated chromatography process development and sample preparation using robotic liquid handling workstations.

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OPUS® RoboColumns® are designed to work with robotic fluid handlers for fully automated parallel chromatography.

Up to 96 RoboColumns® (8 column types in parallel) can be individually arranged on a 96-well array plate according to application requirements.  Resin is contained to form a bed between two filter frits and validated with consideration to resin compression requirements, to ensure high efficiency, good asymmetry and robust chromatography performance.

  • Pre-packed with user-specified chromatography resin
  • Consistently packed with correct axial compression
  • Operating linear flow-rate controlled by dispensing speed of the liquid handling workstation
  • Demonstrated scale-up and scale-down due to constant residence time 
  • Large number of samples per time, ability to multiplex

 OPUS® RoboColumns® are available in the following formats:

Internal Diameter Bed Height Column Volume
cm mm cm mm mL µL
           
0.5 5.0 0.25 2.5 0.05 50
0.5 5.0 0.50 5.0 0.10 100
0.5 5.0 1.00 10.0 0.20 200
0.5 5.0 2.25 22.5 0.45 450
0.5 5.0 2.50 25.0 0.50 500
0.5 5.0 3.00 30.0 0.60 600


Minimum order is one row of 8 OPUS® RoboColumns® with the same resin and column dimension.

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OPUS® RoboColumn®
5 mm ID x 10 mm H; V = 200 µL

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OPUS® RoboColumn®
5 mm ID x 30 mm H; V = 600 µL

Shorten process development time significantly by parallel operation of OPUS® RoboColumns® with robotic liquid handling workstations, implementing chromatography Design of Experiment (DOE) and QbD (Quality by Design).

Compatible with both Tecan and PerkinElmer platforms, the preferred supplier for OPUS® RoboColumn® automation is the Tecan Freedom EVO® robotic workstation.

  • Simultaneous automated step-gradient elution into a 96-well plate
  • Subsequent analysis in an integrated plate reader
  • Flexible software wizard 

The combination of OPUS® RoboColumns® and the Tecan Freedom EVO® robotic workstation delivers a complete integrated platform for state-of-the-art small-scale protein purification.

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  • Convenient, pre-packed columns provided ready to use
  • Flexible, packed with user-specified chromatography resin, multiple column volumes
  • Automated, use with Tecan and Perkin Elmer robotic fluid handlers
  • Fast, process up to 8 RoboColumns® in parallel, shortens development time
  • Proven applications in the industry for over 10 years
  • Cost-effective, reduces resource requirements

Resin and condition screening

Using OPUS® RoboColumn® on a Tecan Freedom EVO® generated up to 96 pseudo-chromatograms (1536 data-points) in 1.5 days when comparing 12 CIEX resins at 8 different pH 3.7-7.0.

  • 8 chromatographic separations in parallel with 16 fractions each in 16 minutes
  • 96 individual buffers in 10 minutes
 
 

Sample preparation / Process analytics

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The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates.  Non-purified samples require sample preparation before analysis can be executed. OPUS® RoboColumns® enable one-step purification of multiple samples in parallel.

Using the OPUS® RoboColumn® on a Tecan Freedom EVO® generates 2mg to 24mg API (Active Pharmaceutical Ingredient) per RoboColumn® run.

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system.  We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

LEARN MORE ABOUT REPLIGEN MANUFACTURING

Plate size

128.3 L x 86.0 W x 14.0 mm H

Plate material

Polyoxymethylene copolymer

Number of wells

96

Column volume

50 µL

100 µL

200 µL

450 µL

500 µL

600 µL

Bed height

2.5 mm

5.0 mm

10.0 mm

22.5 mm

25.0 mm

30.0 mm

Column inner diameter

5 mm

Column material

Polypropylene

Chemical stability

All commonly used aqueous buffers, pH 1 – 14, organic solvents*.  Avoid the use of halogenated organic solvents, hexane

Column storage solution

  • AIEC and CIEC Columns: 20 % ethanol + 150 mM sodium chloride
  • HIC, SEC, Affinity and MMC Columns: 20 % ethanol
  • CHT, CFT Columns (Bio-Rad): 20 % ethanol + 10 mM sodium phosphate, pH 6.8
  • ProSep® Columns(Millipore):1 % benzyl alcohol + 0.1 M sodium acetate, pH 5.1

Recommended storage temperature

  • AIEC, CIEC, HIC, SEC and MMC: +4°C to +30°C
  • Affinity Columns: +2°C to +8°C

Working temperature

+4°C to +30°C

Maximum working pressure

Up to 8.0 bar

Flow velocity

16 – 1000 cm/h**

Labelling

Laser-etched on every individual column body. Label contains:

  • Product number
  • Resin name
  • Column volume in µL

* Note: The chemical stability of the packed chromatography resin has also to be taken into consideration. For more information please refer to the manufacturer’s resin specification.
** The Liquid Transfer menu of the Te‑Chrom™ Wizard enables set up of the flow rate for each individual dispensing and chromatography step (5.1.1.6 Liquid Transfer, Te‑Chrom™ Wizard Software Manual, 396076, en, V1.0).
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