CaptivA_all.jpgThe value choice in Protein A resins, CaptivA® Resin delivers industry-standard results at a fraction of the cost of traditional resins.

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CaptivA Protein A Resin

CaptivA® Protein A Affinity Resin combines an industry standard cross-linked agarose bead with recombinant native Staphylococcal Protein A (rSPA) manufactured by Repligen, the world’s leading manufacturer of recombinant Protein A.

Base Matrix
4% highly cross-linked agarose bead

Particle Size
45-165 μm

Ligand

  • Recombinant native Staphylococcus Protein A (rSPA) expressed in E.coli
  • Recombinant form equivalent to the native Protein A form
  • Contains all five immunoglobin binding domains (E,D,A,B,C)
  • Coupling via reductive amination provides multi-point attachment


Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system.  We focus on the timely delivery of high quality, consistent and robust products, and ensuring business continuity for our customers.

LEARN MORE ABOUT REPLIGEN MANUFACTURING

  • Industry- accepted, in 3 commercial processes and an expanding pipeline of Phase 1-3 processes 
  • High dynamic binding capacity, 30-40 mgs / ml
  • Low Protein A leaching, less than 10 ng/mg IgG 
  • Cost-efficient, one-third the cost of traditional resins, increased savings when pre-packed into OPUS® columns
  • Secure supply

Dynamic Binding Capacity
44 mg hIgG/ mL at 6 min residence time

Protein A Leaching
< 10 ng/mg IgG

Caustic Stability
100 cycles with 0.1M NaOH

Recommended Flow Velocity
30-300 cm/hr

Recommended pH and Temperature Stability
Working: 3-10
Cleaning-in-Place: 2-11
Temperature Stability: 4-40 C

CaptivA® Protein A Affinity Resin delivers high dynamic binding capacity at standard process flow rates and residence times.

CaptivA_binding capacity.jpgDynamic binding capacity was determined for human polyclonal IgG at four different flow velocities providing residence times ranging from 0.5 to 6 minutes. A 5 x 50 mm column was loaded with a 2 mg/ml hIgG solution in PBS pH 7.4. Capacity was determined and reported at 2, 5, and 10% breakthrough. A column bed height between 10-20 cm, loaded at 200 cm/hr provides 6-12 minutes of residence time.

CaptivA® Protein A Affinity Resin retains desired dynamic binding capacity when exposed to stringent washing conditions that reduce standard feed stream contaminants. The CaptivA® recombinant Protein A attachment mechanism creates multiple covalent attachment points between the ligand and agarose bead resulting in superior alkali resistance compared to single point attachment chemistries.

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The human polyclonal IgG dynamic binding capacity was determined following 0.1 N NaOH exposure. A 5 x 50mm column was loaded with a 2 mg/ml hIgG solution in PBS pH 7.4. The loading residence time was 3 minutes. Capacity was determined and reported at 2% breakthrough. After elution, the column was exposed to 0.1 N NaOH for 5 hours.  Five cycles were completed. An exposure time of 25 hours represents 100 x 15 minute CIP cycles.

 

CaptivA® Protein A Affinity Resin multi-point protein chemistry consistently delivers low Protein A leaching.

CaptivA_Protein_leaching.jpgThe human polyclonal IgG binding capacity was determined following each column cycle. Cleaning was performed between cycles with either extra volumes of Glycine pH 3.0 elution buffer or 0.1 N NaOH with a 15 minute contact time. Leached Protein A was measured in the product elution pool from each cycle using the Repligen Protein A ELISA Kit.

Protein A  leaching from CaptivA® Protein A Resin is  consistently

Learn more about Protein A ELISA KiTS

CaptivA® Protein A Affinity Resin is available pre-packed into OPUS® chromatography columns, combining the superior process economics of CaptivA® resin with OPUS® Column ready-to-use convenience and flexible column size options.

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Learn more about OPUS® Pre-packed Chromatography Columns

CaptivA® Protein A Affinity Resin is the most cost-effective chromatography resin available today, delivering industry standard performance at a fraction of the cost of traditional affinity resins, especially when pre-packed into OPUS® chromatography columns.

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LEARN MORE ABOUT OPUS® PRE-PACKED CHROMATOGRAPHY COLUMNS

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