Using Single Use TFF membranes provides increased flexibility for a Contract Manufacturing Organization (CMO) with long term benefits to the processes that use them. Benefits include cost of goods reduction, suite time reduction, and decreased cleaning validation studies leading to a win-win relationship between client and CMO.
Stephanie Wickham performed her graduate studies at the University of Oklahoma Health Sciences Center with a Ph.D in Microbiology and Immunology earned in 2009 with a focus on the determination of amino acids responsible for the binding of a bacterial toxin to its mammalian cellular receptor. During her graduate work, Stephanie gained experience in small scale microbial fermentation, transient transfections of mammalian cell lines, small scale affinity purification, UFDF to concentrate membrane vesicles, and conjugation to fluorescent probes. A post-doctoral fellowship was performed at the University of Oklahoma Health Sciences Center in Cell Biology developing small molecule enzyme inhibitors. During her post-doctoral fellowship, Stephanie gained valuable experience with yeast fermentation, enzyme kinetics, UFDF to concentrate fermentation broth, and affinity purification. Following completion of her post-doctoral fellowship in 2012, she joined Cytovance Biologics in the downstream manufacturing group where she gained the experience with large scale purification and its associated equipment, which led to a transition of the Manufacturing Sciences team. Once in manufacturing sciences, Stephanie became versed in mammalian and microbial fermentation in addition to the downstream. She has led this team since late 2014 and facilitated the successful transfer of more than 25 processes to manufacturing that include antibodies, fusion, enzyme, and cytokines.
Brent is currently the Director of Downstream Manufacturing at Cytovance Biologics. He joined Cytovance in 2012 as an on-the-floor production associate and has since advanced into his current role. Brent’s bioproduction experience ranges across a wide array of molecules (MAbs, enzymes, cytokines, fusions, etc.) from mammalian, microbial and transgenic platforms. Brent received his BS in Biomedical Engineering from Michigan Technological University and Ph.D. in Bioengineering from University of Oklahoma in 2012.