Only Repligen offers ready-to-use devices with ultrapure membrane to provide excellent sample recovery and ultimate convenience - no need for closures, knots, buoys, pontoons, or weights.
HOW TRADITIONAL DIALYSIS WORKS
FLOAT-A-LYZER
The Float-A-Lyzer® is the ready-to-use laboratory dialysis device for 1 ml - 10 ml volumes.
- Uses ultrapure Biotech Cellulose Ester (CE) Membrane
- 3 volume sizes
- 9 molecular weight cut-offs (MWCOs)
Part NUmbers and specifications
EASY TO USE
Ready to use out of the box, the leak-proof screw-on cap provides easy sample access with included pipette without risk of needle punctures
ULTRAPURE MEMBRANE
Biotech Grade Cellulose Ester (CE) membrane tubing prevents polarization and membrane fouling, achieves high sample purities; no heavy metals and sulfide contaminants
unique design
Included floatation ring improves sample buoyancy and vertical orientation during dialysis, design allows multiple samples to be dialyzed in the same reservoir
total sample recovery
95-98% sample recovery while maintaining 98% sample purity
Broad MWCO Range
3 volume sizes, 9 color-coded molecular weight cut-offs (MWCOs)
Three easy steps
-
Rinse with water
-
Load sample
-
Dialyze
| 3 Volume Sizes | 1 ml | 5 ml | 10 ml |
|---|---|---|---|
| Working volume | 0.8 ml - 1.3 ml | 4.0 ml - 5.5 ml | 8.0 ml - 11.0 ml |
| Total length | 5 cm | 10 cm | 16 cm |
| Membrane diameter | 10 mm | 10 mm | 10 mm |
| Top piece diameter | 23 mm | 23 mm | 23 mm |
| Flotation ring | 38 mm | 38 mm | 38 mm |
| 9 MWCOs | Color Code |
|---|---|
| 0.1 - 0.5 kD | Green |
| 0.5 - 1 kD | Orange |
| 3.5 - 5 kD | Black |
| 8 - 10 kD | Yellow |
| 20 kD | Red |
| 50 kD | Violet |
| 100 kD | Blue |
| 300 kD | Amber |
| 1000 kD | Pink |
| Membrane type | Biotech grade Cellulose Ester (CE) |
| Screw-on cap | Open and close for in-process sampling |
| Color-coded for MWCO | |
| Polypropylene | |
| Silicon O-ring | Leak proof and resealable |
| Flotation ring | Device body inserts through center to maintain buoyancy in buffer |
| Polyethylene | |
| Top/Bottom piece | Polycarbonate |
| Potting | Polyurethane |
| Sample loading | Disposable pipette for 5 and 10 ml (included) Pipette tip for 1 ml (not included) |
| Package/Quantity | Dry with glycerol, 12/package |
Micro Float-A-Lyzer®
The Micro Float-A-Lyzer® is a pre-assembled device designed for easy and efficient dialysis of very small sample volumes (100-500 µl).
- Uses ultrapure Biotech Grade Cellulose Ester (CE) tubular membrane
- 2 volume sizes
- 7 molecular weight cut-offs (MWCOs)
Part Numbers and specifications
EASY TO USE
Ready to use out of the box, the individual syringe allows for easy sample access, loading and retrieval without risk of needle punctures
ULTRAPURE MEMBRANE
Biotech Grade Cellulose Ester (CE) membrane tubing prevents polarization and membrane fouling, achieves high sample purities; no heavy metals and sulfide contaminants
unique design
Can stand upright on benchtops, or float and self-orient during dialysis, units can interlock for simultaneous dialysis of multiple samples
total sample recovery
95-98% sample recovery while maintaining 98% sample purity
Broad MWCO Range
2 volume sizes, 7 color-coded molecular weight cut-offs (MWCOs)
| 2 Volume Sizes | 100-200 µl | 400-500 µl |
|---|---|---|
| Height | 4.4 cm | 6.3 cm |
| Width | 4.5 cm | 4.5 cm |
| Thickness | 1.9 cm | 1.9 cm |
| Membrane flat width | 10 mm | 10 mm |
| Membrane diameter | 6.4 mm | 6.4 mm |
| 7 MWCOs | Color Code |
|---|---|
| 0.1 - 0.5 kD | Green |
| 0.5 - 1.0 kD | Orange |
| 3.5 - 5 kD | Black |
| 8-10 kD | Yellow |
| 20 kD | Red |
| 50 kD | White |
| 100 kD | Blue |
| Membrane type | Biotech Grade Cellulose Ester (CE) |
| PP Luer-Lok cap | Open and close for in-process sampling Color-coded for MWCO Leak proof and re-sealable |
| Body piece | Seals top/bottom ends of membrane Buoyant and self-orienting Polycarbonate |
| Potting | Polyurethane |
| Sample loading | 1 ml syringe (included) |
| Packaging | 12/package |
tube-a-lyzer®
The Tube-A-Lyzer® is a ready-to-use, gentle dialysis separation device that combines convenience and disposability with the efficiency of dynamic dialysis.
- Uses Biotech Grade Cellulose Ester (CE) membrane tubing
- 6 molecular weight cut-offs (MWCOs)
- 2 volume sizes
part numbers and specifications
EASY TO USE
Ready to use, the dialysis device provides easy access for loading, recovering and in-process testing. This closed, high efficiency device offers high concentration dialysis without manual buffer changes
ULTRAPURE MEMBRANE
Biotech Grade Cellulose Ester (CE) membrane tubing prevents polarization and membrane fouling, achieves high sample purities; no heavy metals and sulfide contaminants
faster
Increases the rate of dialysis and reduces overall dialysis time from 1-2 days to 4-12 hours
Sterile
Non-pyrogenic device, can be sterilized (25 kGy gamma irradiation).
total sample recovery
95-98% sample recovery while maintaining 98% sample purity
unique design
Self-contained, disposable device incorporates a semi-permeable membrane tubing that separates the sample chamber from the surrounding flow-through buffer chamber
| 2 Volume Sizes | 8 - 10 ml | 25 - 30 ml |
|---|---|---|
| Buffer chamber volume | 50 - 55 ml | 120 - 130 ml |
| Total length | 23 cm | 50 cm |
| Total diameter | 2.2 cm | 2.2 cm |
| Membrane effective length | 14 - 16 mm | 36 - 38 mm |
| Membrane diameter | 1.0 mm | 1.0 mm |
| 6 MWCOs |
|---|
| 0.1 - 0.5 kD |
| 3.5 - 5 kD |
| 8-10 kD |
| 20 kD |
| 50 kD |
| 100 kD |
| Membrane | Biotech grade Cellurose Ester membrane (CE) |
| Sample chamber | Polycarbonate top and bottom piece Polyurethane potting Female Luer-lok sample port Polypropylene male Luer-lok cap |
| Buffer chamber | Polycarbonate housing Polycarbonate end caps 6 mm (3/16 inch) hose-barb inlet / outlet ports |
| Dynamic pump rate | 10-20 ml/min (should be optimized for application) |
| Packaging | Dry packaged with 20% glycerin |
accessories
These polystyrene cylindrical reservoirs provide the optimum environment for laboratory dialysis. Stir bar is included.
| 2 Volume Sizes | 600 ml | 2400 ml |
|---|---|---|
| Dialysis device | up to 1 ml | any size |
| Tubing flat width | up to 16 mm | up to 80 mm |
Spectra/Gel® makes the concentration of protein sample solutions quick and efficient. Made of polyacrylate-polyalcohol it has the property of expanding as it absorbs water. The high molecular weight polymer can be used with all Repligen dialysis membranes without contaminating the sample. After dialysis, remove the dialysis tubing and coat it with Spectra/Gel® . Water and smaller molecules diffuse through the membrane and are absorbed by Spectra/Gel® Absorbent. Spectra/Gel® is easy to remove after absorbancy.
- High water absorbent capacity
- Easy to use with any MWCO dialysis membrane
- Easy to concentrate small molecular weight molecules
- Easy to remove
- Cost effective
- Convenient for sample concentration with Float-A-Lyzer® G2
The length of time needed for concentration will depend on the final volume, the MWCO, the surface to volume ratio of the membrane tubing and the amount of Spectra/Gel® Absorbent. After dialysis in a Spectra/Por® Float-A-Lyzer G2 with a 20 kD MWCO, the volume can be reduced from 5 mL to 0.5 mL in 60 minutes with 100 grams of the Spectra/Gel® Absorbent.
Easy-to-use dialysis products such as Spectra/Por® Float-A-Lyzer G2 can be used with Spectra/Gel® to concentrate samples.
Designed with durability, reliability, and safety in mind, the Spectra/Por® Magnetic Stirrer has a precision, high-speed control system—ranging from 60 - 1500 RPM—with a continuously smooth revolution pattern. The technically advanced PCB (Printed Circuit Board) eliminates electrical overload and regulates energy distribution. A bearing-type motor maintains gentle revolution over the lifetime of the equipment. The Spectra/Por® Magnetic Stirrer features a heat-resistant, anti-corrosive aluminum body with a high-quality powder coating. A ceramic coated stainless steel top plate provides excellent chemical resistance, especially against strong acids and bases.
- Small footprint, ideal for benchtop workspace
- Ceramic coated stainless steel top plate suitable for large glassware
- Responsive turn knob for precise adjustment of speed
- Includes support rod, excellent for mounting thermometers
- CE Certified
| Speed Range | 60-1500 rpm |
| Controller | Electronic solid state |
| Material | Ceramic coated stainless steel |
| Overall dimensions | 8.5 x 10 x 4.5 in | (21.5 x 25.4 x 11.4 cm) |
| Plate dimension | 7.3 x 7.1 in | (18.5 x 18 cm) |
| Maximum power consumption | 500 watt, 3.0 amp |