The KrosFlo® KML System is designed for upstream bioreactor perfusion using tangential flow filtration (TFF), with a single-use, non-pulsating, levitating magnetic pump.
• 1.5L - 100L volume
Featuring a low shear magnetic levitating pump for improved cell viability, the KrosFlo® KML TFF System is ideal for upstream perfusion managing 1.5L to 100L working volume bioreactors. Combined with gamma sterilized single-use ProConnex® Flow Paths, the KrosFlo® KML System delivers high protein production during lengthy TFF perfusion operations.
The KrosFlo® KML System enables faster process development facilitating multiple tests and processes, accurate process definition and execution consistency. Fully integrated and ready to use out of the box, the system includes precise and reproducible control and documentation of TFF processes.
KrosFlo® KML TFF SYSTEM
The KrosFlo® KML TFF System is a completely integrated system with a color touchscreen to display key parameters. There are visual and audible alarms with safety interlocks to ensure process parameters are maintained. The system comes with two auxiliary peristaltic pumps, two auxiliary scales for precise the management of the flow dynamics are sold separately. A retentate control valve manages TMP by adjusting the pinch distance between the plunger and the tubing, and an ultrasonic retentate flow meter that clamps on the tubing. To prevent cells from settling and forming a gel layer on the membrane lumen, the KrosFlo® KML System adjusts through a PID control loop from the flow meter. Further advancements include automatic back pulsing features and USB port for real time data collection.
Fully integrated
Includes filters, pumps, scales, process monitors, flow meters, software and graphic display
Multiple modes
TFF Perfusion, 5 additional TFF modes, 1 manual mode. Scale sizes selected by the user
Configurable
2 filter module configurations, 4 membrane chemistries, 3 effective filter lengths
easy User controls
Pressures, flows, weights and process overview with end user set-point functionality.
Ready to use
Out of the box functionality, short time to system proficiency
Scalable
Compatible with KrosFlo® TFF perfusion systems from process development to commercial manufacturing
Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.
CONFIGURABLE ProConnex® FLOW PATH
Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration. Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.
Automated Process Control Modes
Perfusion mode, five system process control modes and one manual operation mode are available with the KrosFlo® KML TFF System.
| Mode | Primary Application | ||
|---|---|---|---|
| Perfusion Mode | Cell Culture perfusion | ||
| Manual Mode | Simple concentration of the process solution | ||
| C | Concentration Mode |
Simple concentration of the process solution |
|
| C/D | Concentration / Diafiltration | Concentration and then diafiltration of the process solution in succession | |
| C/D/C | Concentration / Diafiltration / Concentration | Diafiltration of the process solution with one buffer before concentration of the process solution a second time | |
| C/D/D/C | Concentration / Diafiltration / Diafiltration / Concentration | Diafiltration of the process solution with two different buffers in succession before concentration of the process solution a second time | |
| CFC | Constant Feed Concentration | Concentration of the solution while maintaining a constant feed weight | |
Compatible Filter Modules
| Primary Application |
Volume | Inlet/ Outlet | Side Port |
Scale Length |
Effective Length (cm) | Total Length (cm) |
Surface area |
|
|---|---|---|---|---|---|---|---|---|
MiniKros Sampler |
||||||||
 |
Batch TFF of small volumes | 3 L -15 L | 0.75" TC | 0.75" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 47 70 |
490 – 1000 cm2 1000 - 2200 cm2 1600 - 3400 cm2 |
MiniKros |
||||||||
 |
Pilot-scale TFF | 5 L - 50 L | 1.5" TC | 0.75" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 46 70 |
0.16 - 0.31 m2 0.32 - 0.65 m2 0.51 - 1.0 m2 |
Scalable from benchtop to production
The KrosFlo® family of TFF Systems for perfusion cell culture supports scale up from process development to commercial manufacturing.
KrosFlo® KML System
1.5L - 100L
KrosFlo® KPS Systems
500L - 5000L
Components
- Flow Range 350 ml/min to 13 LPM at 7000 RPM @ 15 psig (1 bar)
- 0-9000 RPM range with 300 watt power supply
- FDA approved materials, USP Class VI, Animal BSE/TSE Free Material
- PLC with color 8.4" touchscreen HMI
- 0-40° C Operating Range
- CE/UL/cUL marked
- Automation Direct DL06 PLC with 800x600 Resolution HMI
- Splash-proof
Integrated Detachable
Pro-Connex® Flow Path Stand
Designed to hold gamma sterilized single-use Pro-Connex® Flow Path assemblies
2 Integrated Peristaltic Pumps
- Flow ranges from 0.16 to 347 ml/min
- Permeate pump sequenced for back-pulsing in the Perfusion mode
- RS232 communication
- Integrated power within single connection to KML
Retentate Control Valve
- Non-invasive to process stream
- 4-20 mA control
Ultrasonic Single Pass Retentate Flow Meter
- Measures flow up to 20LPM +/- 5%
- Calibrated for Dow Pharma tubing at 35°C
