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AVIPure® Albumin Affinity Resin

Discover a high performance affinity chromatography resin for the primary capture of human albumin from complex feedstocks.

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When it comes to purifying small albumin fusion proteins or recombinant albumin, the choice of chromatography resin can make all the difference. That's where AVIPure Albumin Affinity Chromatography Resin steps in, setting a new standard in protein purification. With it's innovative design and exceptional performance, AVIPure offers a unique solution for your purification needs.
 

The AVIPure Albumin Resin Difference

  • Higher Dynamic Binding Capacity: AVIPure Albumin's dynamic binding capacity sets it apart from other commercially available albumin affinity resins. With a higher binding capacity, you can load more sample and obtain greater yields in each purification run. This not only enhances the efficiency of your process but also maximizes your overall productivity
  • Ease of Cleaning: AVIPure Albumin stands out from the competition for its stability and simple cleaning protocols. Unlike other affinity resins on the market, our resin can be cleaned using 0.5 molar sodium hydroxide
  • Exceptional Reusability: We take pride in the longevity of AVIPure resins. Our data demonstrates that this resin can be used for up to 90 cycles with no noticeable impact on its performance. This remarkable durability not only ensures consistent performance but also significantly reduces your cost of goods. With AVIPure, you'll get more value from every liter of resin
  • High Flow Rates: We understand the need for efficiency in protein purification. AVIPure features a cutting-edge affinity ligand attached to a larger 70-micron base bead, allowing you to run your purification process smoothly at higher flow rates. This means you can achieve your purification goals faster, saving valuable time and resources
  • Bind and Elute Mode: Our resin is specifically designed for bind and elute mode, streamlining your workflow and ensuring maximum yield in every purification run. Say goodbye to inefficient processes with low yields and embrace a simpler, more effective way to purify your target proteins while achieving high purity and yield
     

Resin Design

AVIPure Albumin chromatography resin consists of small recombinant ligand coupled to a 70 micron hardened agarose base bead manufactured free of animal derived components. It features a nanomolar affinity to human albumin and fusion proteins. The binding site is distal to both the N and C termini, reducing the risk of steric hindrance with a fusion partner.

AVIPure Albumin Affinity Resin Performance Characteristics

 

 

AVIPure Albumin Affinity Resin 

Affinity Resin A

Affinity Resin B

Affinity Resin C
Dynamic Binding Capacity

40 mg/mL, 4 min RT, pH 7.4 

>24 mg/mL, 4 min RT

≥18 mg/mL at pH 7

15 mg/mL
Elution

pH 3 or pH 8-9 with additives

50 mM sodium phosphate, 2 M NaCl, pH 7

50 mM sodium phosphate, 2 M NaCl, pH 7

pH 3 or neutral:
2 M MgCl2, or 1 M NaCl+0.5 M arginine
Particle Size

~70 µm

~75 µm

~90 µm

~50 µm
Pressure Flow

375 cm/h at 20 cm bed height

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm 

200-400 cm/h
Cycling / CIP

0.1–0.5 M NaOH

0.5 M NaOH

0.1–0.5 M NaOH

No NaOH

 

 

AVIPure Albumin Affinity Resin Characteristics
 

Affinity (KD)

Target

Fusion

Size by SEC

Affinity

HSA

-

66 kDa

3 nM

AF#1

N-term

300 kDa

<1 nM

AF#2

C-term

120 kDa

2 nM

AF#3

C-term

120 kDa

<1 nM

AF#4

C-term

150 kDa

1 nM

AF#5

C-term

76 kDa

<1 nM

AVIPure Albumin shows even higher capacity at lower pH

  • 3.6 mL column (0.66 x 10cm)
  • 8 minute load residence time 

AVIPure Albumin affinity resin has high NaOH tolerance for superior cleaning and resin stability 

AVIPure Albumin affinity resin tolerates >45 hours of incubation in 0.5 M NaOH.

  • 0.3 x 5 cm column (0.35 mL)
  • 4 mg/mL HSA load concentration
  • 4 min residence time

Pressure flow curve

  • 20% lower backpressure at 300 cm/hr than the 50 micron base bead
  • Maximum recommended flow
    • 15 cm = 450 cm/h
    • 20 cm = 375 cm/h
    • 25 cm = 275 cm/h

Why choose AVIPure?

The choice of chromatography resin is a critical decision in protein purification. AVIPure Albumin Affinity Chromatography Resin offers a range of benefits that make it the superior choice for researchers and scientists. Whether you're working with small albumin fusion proteins or recombinant albumin, our resin's high flow rates, ease of cleaning, and exceptional dynamic binding capacity all work together to enhance the efficiency of your purification process.

Furthermore, our commitment to sustainability and cost-effectiveness is evident in AVIPure's impressive reusability. With the ability to withstand up to 90 purification cycles, you can count on AVIPure to consistently deliver the results you need while reducing your operational costs.

1 L of resin cycled 800 times produces approximately 28 kg Albumin when loaded to 80% capacity each cycle. Additionally, users are able to achieve even higher productivity if the resin is used during continuous chromatography. 

 

Experience the AVIPure Advantage

AVIPure Albumin Affinity Chromatography Resin is more than just a purification tool; it's a game-changer for your research and production needs. Join the growing community of scientists and researchers who trust AVIPure to streamline their protein purification processes and elevate their results.
Make the smart choice for your research. Contact us today to learn more about AVIPure and how it can benefit your purification workflow. 

 

Request a Sample

Complete this form to request an affinity resin sample. A technical specialist will contact you shortly to arrange the resin evaluation.

 

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