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Minimize Risk With Rapid At-Line Concentration Measurement in Downstream Bioprocessing Applications (Webinar+Write Up)
In this webinar, we will review VPT and how it can be used in various applications from protein therapeutics to gene therapy. We will share real-world case studies demonstrating how companies have integrated the SoloVPE® System into their manufacturing workflow for release and in-process testing. Speaker: Paul Mania
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LONG® EGF
LONG® EGF is a recombinant analog of human epidermal growth factor (EGF) used in regulatory approved cell-based therapies.
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LONG® R³ IGF-I
LONG® R³ IGF-I (LONG R³) is a recombinant analog of human insulin-like growth factor-I (IGF-I) engineered for enhancement of cell culture performance.
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Fast Facts: Streamlining at-line and in-line concentration measurements with the Variable Pathlength Technology approach
Watch the video or read the poster to learn: What are the challenges of UV spectroscopy in pharmaceutical analysis? What is variable pathlength technology and how does it work? How can variable pathlength technology be used for at-line and in-line GMP applications? Speaker: Ramsey Shanbaky
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Single-use Containment Solutions
Single-use flexible and rigid containment solutions tailored for bioprocessing needs. Optimize liquid handling with 2D and 3D bags constructed from a robust-high-purity film platform to bottles and carboys.
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Go with the Flow: CTech FlowVPE System in combination with MALS for Real Time Molecular Weight (RT-MW) Applications
Multi Angle Light Scattering (MALS) can provide the real time molecular weight (Mw) of a pure solute or the average Mw of a solute matrix if the total concentration of the species is also available in real time. Analytical experiments with low protein concentration can use conventional fixed path length UV sensors, however these become saturated at the higher concentrations of manufacturing processes. The FlowVPE System enables the real time measurement of protein content in a wide concentration range, which can be coupled to MALS for RT-MW measurements. Besides a more agile process development, RT-MW can enable adaptive processing decisions and inform on product deviations Speaker: Andres Martinez-Roche
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Optimizing E. Coli cell growth performance with in-line, real-time OD600 (abs/mm) monitoring during the fermentation process
This study proposes utilizing an inline- variable pathlength spectrophotometer in a recirculation loop during fermentation to monitor and optimize the growth of E. Coli cells in real-time. This provides distinct advantages vs traditional methods due to the constant modification of path lengths to create a slope-based solution that is always within the linear range of the equipment with no buffer correction required. The growth curve is tracked in real-time using the slope (Absorbance/mm) at 600nm to measure cell density and to optimize growth performance of E. Coli cells. This will serve as a proof-of-concept for the optimal cultivation of E. Coli and production of pDNA. Speakers: Tanja Buch, Ramsey Shanbaky
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