Ultrafiltration / Diafiltration

Used in nearly every biotherapeutic process, ultrafiltration/diafiltration (UF/DF) is a critical purification process, using tangential flow filtration (TFF), prior to final drug formulation.

Ultrafiltration or UF (sometimes referred to as concentration) involves separation of components, on the basis of molecular weight and size, to isolate the biologic of interest. Diafiltration or DF (sometimes referred to as buffer exchange) involves the addition of buffer such that the liquid surrounding the target is exchanged to one that is preferred for storage stability. Several UF/DF operations can be connected in a series to perform multiple UF/DF steps until the desired purity levels are reached.

Repligen set new standards in UF/DF with a complete range of Tangential Flow Filtration (TFF) Systems from bench to production scale with walk-away automation, including single-use flow paths, market-leading hollow fiber and flat sheet filters, and in-line process monitoring.

Repligen solutions help overcome key challenges in UF/DF, with hands-on process and implementation consultation from global Field Applications Specialists.

Speak to an expert

Ultrafiltration/Diafiltration (UF/DF) Unit Operation

Click on the workflow links below to find productivity and throughput solutions you can implement today. 
Repligen workflow solutions include hardware, software and consumables that scale from process development to large-scale GMP manufacturing levels.

KrosFlo® TFF Systems

Turnkey systems for tangential flow filtration (TFF), KrosFlo® Systems are compatible with single-use hollow fiber filters and/or flat sheet cassettes, modernizing how UF/DF is performed at every stage from process development to large-scale manufacturing.

ProConnex® Flow Paths for Systems

Expertly designed plug-and-play flow path
 

ProConnex® Flow Paths for TFF and Chromatography processes are single-use and engineered for optimum performance, reproducibility and time savings. System specific flow paths provide speed and reliability. Configured flow paths prioritize process requirements and sterility. ​

Hollow Fiber Filters

Ideal for ultrafiltration, diafiltration and microfiltration, single-use hollow fiber filters for tangential flow filtration are packed in housings engineered to handle high pressures, with a choice of fiber chemistries and sizes from process development to manufacturing.

TangenX® Flat Sheet Cassettes

First-to-market with single-use TFF flat sheet cassettes, TangenX® TFF technologies are reliable, scalable, and cost-effective solutions for efficient concentration and diafiltration.

Fluid Management

Repligen offers innovative, designed-for-purpose storage and transfer solutions for fluid management within and between unit operations. These include flow paths, tubing, valves and gaskets, bottles and containers, totes, carts and cleanroom process equipment.

CTech™ FlowVPX® System

In-line GMP testing

Designed to meet the rigors of regulatory GMP requirements, the new CTech FlowVPX® spectrophotometer system offers reliable real-time results with integrated ease for concentration measurements during every stage of the downstream GMP process.

KrosFlo® KR2i RPM™ System

TFF with In-Line Protein Concentration Management

Monitor concentration in real time during your UF/DF run. Featuring in-line protein concentration management, control your process with the power of the KrosFlo® KR2i TFF System and insights from the integrated CTech™ FlowVPX® System.

Customer First.

Support is part of the Repligen DNA. Our goal is to provide exceptional customer experience, and to support the efficient and successful adoption and implementation of all Repligen products and services.

  • Field Application Support
  • Customer Service
  • Field Service Engineers

Have a question?