Protein Characterization
ZipChip CE-MS delivers rapid, high-resolution analysis of critical quality attributes—including titer, aggregation, and charge variants—directly from complex cell culture samples. With rapid at-line capabilities, you gain actionable insight at the point of need, enabling informed clone selection, earlier process optimization, and a more efficient path to consistent product quality.
Skip The Hard Part
We know characterization of proteins isn’t always easy. That’s where ZipChip comes in. Assay kits take the guesswork out of method development, and ZipChip rarely flinches at matrices with detergent and salt, so you don’t have to waste time on sample prep. There’s no wonder CE-mass spec was used to sequence the human genome
Really Know Your Product
ZipChip’s charge variant assay alone gives you more information than other techniques. Same goes for other characterization assays. Whether you’re running intact charge variant analysis, reduced analysis, or peptide mapping, ZipChip works at every level
Protein Characterization Applications
ZipChip helps analytical scientists take a load off with simple solutions for different protein characterization workflows. And its assay-defined kits mean there’s no method development ⎯ no matter what.
Charge Variant Analysis
Combine native separation with MS data and easily observe protein modifications at native protein level. Charge variant analysis techniques like cIEF and CEX are great, but just knowing you have variants means you still don’t have a complete picture. ZipChip separates variants at the same or better resolution as other methods. Here’s the big deal: because ZipChip uses mass spec for identification, you know exactly WHAT they are too.
Intact Mass Analysis
ZipChip gives you high quality spectra, super-fast analysis times, and greater insight for in-process and routine characterization. But the best bit is ZipChip doesn’t care what’s in your matrix, so you don’t have to waste time removing detergents and de-salting. You can even measure samples straight from your bioreactor ⎯ just dilute and shoot!
Peptide Mapping
Peptide mapping with ZipChip is quick and easy. It also provides an orthogonal approach to LC for separating tricky analytes like conjugated peptides, complex glycopeptides, isomerized residues, and degradants. Multi-attribute methods (MAM) are gaining popularity in the biopharmaceutical industry, but long LC gradients are often needed to accurately measure multiple CQAs. ZipChip helps you avoid that bottleneck with sample runs that take anywhere from 1-15 minutes.
Subunit & Antibody Drug Conjugate Analysis
Get matrix-friendly, domain-specific info with baseline-resolved mAb subunits in <5 mins, plus glycoform resolution for Fc glycans. You don’t waste time switching between methods either. ZipChip makes things simple by letting you do multi-characterization (intact, reduced/subunit, mapping) with the same kit. Just adjust your MS parameters and keep going!
CE mass spectrometry
ZipChip
Pop ZipChip on your mass spec as the source. It separates your samples and electrosprays them into your mass spec for analysis. There’s no optimization to do or connections to make. Click the chip in place and you’re off. Ready to go assay kits are optimized for each application, all you do is dilute-and-shoot.
The ZipChip is like a super-smart, fast sorter – ideal for protein characterization. It separates proteins quickly so we can see and count them easily. It works by using a tiny channel (capillary) and electricity to sort proteins based on their size and charge before they are analyzed. Traditional methods can be slow and need a lot of steps whereas the ZipChip can analyze your samples in minutes instead of hours with minimal sample preparation and method development. The ZipChip is like having a fast pass – it gets the job done quicker and needs less sample, making the whole process of counting proteins much more efficient.