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KrosFlo® RS TFF Systems
The KrosFlo® RS are a series of tangential flow filtration (TFF) systems engineered for an era of new modalities. These systems are single-use and cGMP compliant with features like end-to-end automation, minimal hold-up volumes, and effortless scalability. Support automated ultrafiltration and diafiltration (UF/DF) processes for batch volume ranges of 35mL to 2000L.
product features
When Every Drop Counts
Minimize hold-up volumes and reduce batch variation with valve block technology and end-to-end automation.
Enhanced Process Control
Experience intelligent mixing and recipe customization with Tulip tank and intuitive recipe-driven software platform.
Scalable Solution
Scale effortlessly from 1/8” to 1” while maintaining process parameters.
Bench scale TFF meets cGMP production
Introducing the KrosFlo® RS 10
Meet the first bench scale TFF system built specifically for cGMP production with true end to end automation.
The new KrosFlo RS 10 takes all of the components, controls, and capabilities of our highly compliant, commercial manufacturing large-scale TFF systems and brings it down to bench scale.
One feature unique to the RS 10 is the ability to choose your own feed pump upon ordering to better fit your process needs.
- Centrifugal
- Diaphragm
- Peristaltic
Automation Redefined
While many traditional single-use TFF systems claim to be “automated” they often require frequent manual intervention by operators due to the limited number of controlled valves available. The more controlled valves throughout the system’s flow path, the greater the degree of automation capability.
With significantly more controlled valves than any other TFF systems on the market, the KrosFlo® RS enables users to truly automate the entire process, end-to-end, significantly reducing the risk of human error and inconsistencies from batch-to-batch. Automation beyond UF/DF- that’s end-to-end.
- WFI/Buffer Flushing
- Integrity testing
- Fed-Batch Concentration
- Product Recovery
- Clean-in place
- NBP testing
Recovery Maximized
Traditional hose barb assemblies found on most TFF system flow-paths space valves far apart increasing total hold-up volume and creating dead-legs which limits concentration capabilities. The ProConnex® flow paths featured on KrosFlo® RS are made up of injection molded tubing connections that fit precisely within the recesses of the system’s valve blocks.
The valve block technology allows the RS to cluster a set of valves close together for automated and optimized concentration.
These features and more make these the TFF system of choice for mRNA and gene therapy manufacturers.
Full Process Control Unlocked with KlariFi™
The RS series is powered by the KlariFi™ system control software, an intuitive recipe-driven platform that unlocks the full potential of your TFF run. Use the point and click recipe editor to build from scratch or to customize from an extensive library of pre-built templates.
Enjoy ease of navigation with a flow diagram-based interface that mirrors your system’s hardware. Process controls, settings, and data are available at a glance with the ability to visualize, pause and adjust during a run without compromising batch integrity. Best of all, KlariFi™ is built to enable CFR 21 part 11 and GAMP 5 compliance.
- Intuitive point and click interface
- Windows-integrated access control
- Transfer recipes between systems
- Secure batch reports
- Customizable data trending
Intelligent Mixing Even at the Lowest Working Volume
Innovative tulip tank technology delivers hold-up volumes as low as 1.5L with active mixing.
As fluid levels drop, the dual impellers independently adjust speed to reduce harmful surface interaction while automated valves adapt retentate flow to avoid damage upon entering the tank.
Tulip Tank cart available with KrosFlo® RS 30, 40, 50
Filter Flexibility
Expand your application usage by using the KrosFlo® RS with flat sheet cassettes or hollow fiber filters. Swap between filter types in less than 10 minutes.
Repligen offers the broadest range of membrane formats in the market with TangenX®Cassettes and Spectrum® Hollow Fiber filters.
Proven in Production
Forge Biologics improves pDNA processing efficiency by ~300% with the KrosFlo RS 20 TFF System
Forge Biologics operates one of the largest dedicated gene therapy manufacturing facilities in the world, with 20 custom-designed cGMP suites. When their process development team set out to optimize plasmid DNA purification for their gene therapy pipeline, they chose the KrosFlo RS 20 as their cGMP TFF platform.
The challenge
Forge needed to reduce pDNA processing time without compromising supercoiled DNA quality or RNA clearance, while establishing a scalable workflow from process development through commercial manufacturing.
The approach
Working with Repligen's TFF applications team, Forge evaluated both TangenX flat sheet cassettes and Spectrum hollow fiber filters on the KrosFlo RS 20. They optimized TFF operating parameters using the KrosFlo KR2i TFF system at bench scale, then transferred the process directly to the KrosFlo RS 20 for cGMP production.
The results
- Improved efficiency by ~300% in pDNA processing time through optimized TFF parameters and enhanced automation
- Enhanced RNA removal without additional purification steps
- Seamless scale-up to large volume manufacturing
KrosFlo RS TFF System Overview Videos
New to the KrosFlo RS Systems? Watch TFF experts demonstrate live equipment and guide you through key features.
KrosFlo RS 10 TFF System Overview
Join Lucas Smith as he highlights Repligen’s latest innovation in bench-scale TFF technology, the KrosFlo® RS 10 TFF system. Designed to streamline cGMP manufacturing for small batch therapeutics, the KrosFlo® RS 10 enables operators to choose their preferred feed pump, automate all process steps, and maximize yields. In this overview video Lucas will discuss system features, process flow, and network integration capabilities.
KrosFlo RS 30 TFF System Overview
In this video Lucas Smith walks you through the KrosFlo RS® 30 TFF System, Repligen’s integrated solution to empower cGMP manufacturers with end-to-end automation and optional real-time concentration control. The system comes with 1/2” line sizes, a membrane area ranging between 1 and 5 m2 and is designed to provide you with everything needed for cGMP compliance.
ProConnex Flow Path Installation on KrosFlo RS 30 TFF System
Intuitive and done in a matter of minutes, Lucas Smith provides a step-by-step installation of the ProConnex® flow path onto the KrosFlo® RS 30. The process outlined in this brief overview is applicable to all KrosFlo RS systems.
KrosFlo RS 50 TFF System Overview
In this video, Lucas Smith gives us a closer look at the innovative KrosFlo® RS 50 TFF System by Repligen. Discover unique features such as intelligent mixing and end-to-end automation. This single-use TFF system supports 1” line size tubing and membrane areas ranging between 5 and 20m2.
Scale up Streamlined
Designed as a single standardized platform and not as standalone systems, KrosFlo® RS share the same industrial grade components and automation capabilities across the entire system series. With the same software, hardware, controls and cGMP compliance built into every system, challenges in scale-up are significantly reduced, allowing you to confidently maintain your process as you scale.
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| System | RS 10 | RS 20 | RS 30 | RS 40 | RS 50 |
| Tubing Diameter | 1/8" | 1/4" | 1/2" | 3/4" | 1" |
| Flatsheet | 0.01 - 0.1 m2 | 0.1 - 0.5 m2 | 1 - 5 m2 | 1 - 10 m2 | 5 - 20 m2 |
| Hollow Fiber | 0.02 - 0.6 m2 | 0.05 - 1.0 m2 | 0.05 - 5 m2 | - | 4 - 34 m2 |
| Recirculation Pump (Diaphragm) |
0.5 LPM | 3.4 LPM | 20 LPM | 41 LPM | 83 LPM |
| Centrifugal | 0.73 LPM | - | - | - | - |
| Peristaltic | 0.34 LPM | - | - | - | - |
Upgrade to RPM™ for Real-time Concentration Control
Integrated with the CTech™ FlowVPX® in-line spectrophotometer, unlock advanced analytical capabilities based on real-time concentration data. Reduce variability, offline sampling and dilution steps.
- Greater control and reproducibility
- Increase efficiency and yields with reduced cycling
- Reduce risk with real-time concentration data
Explore the System Components
KrosFlo® RS single-use Tangential Flow Filtration (TFF) systems incorporate the most advanced engineering principles alongside unique hardware to achieve unprecedented automation, hold-up volume and process control.
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KrosFlo® RS 10 TFF System
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KrosFlo® RS 20 TFF System
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KrosFlo® RS 30 TFF System
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KrosFlo® RS 40 TFF System
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KrosFlo® RS 50 TFF System
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KrosFlo RS TFF systems are a series of five single-use, cGMP-ready tangential flow filtration systems by Repligen, designed for fully automated UF/DF processing across batch volumes from 25 mL to 2,000 L.
The KrosFlo RS TFF system is different because it enables true end-to-end automated UF/DF with a highly actuated valve architecture that reduces manual steps and improves process consistency. Valve block technology and ProConnex injection-molded flow paths help minimize hold-up volume and dead legs, while the Tulip Tank supports effective mixing at low working volumes (down to 1.5 L). The KrosFlo RS can also integrate with the PATsmart™FlowVPX® system to use real-time in-line concentration measurements for automated transitions and concentration-based endpoint control (instead of relying only on VCF).
The KrosFlo RS TFF System is designed for automated UF/DF across multiple modalities, including monoclonal antibodies (mAbs), recombinant proteins, vaccines, and cell and gene therapies (AAV, lentivirus, CAR‑T, and RNA-based therapies such as siRNA and mRNA). For more challenging applications such as antibody drug conjugates (ADCs) and high‑concentration mAbs, built-in control capabilities help enable reliable UF/DF while supporting product quality.
Yes. All KrosFlo TFF RS systems feature 304L stainless steel construction, single-use flow paths, and KlariFi software that enables CFR 21 Part 11 electronic records and signatures, GAMP 5 compliance, and secure batch reporting.
KrosFlo RS TFF systems are compatible with TangenX flat sheet cassettes, Spectrum hollow fiber filters, and most third-party filter options. Users can swap between filter types in under 10 minutes.
All five KrosFlo RS TFF system models (RS 10 through RS 50) share the same software, hardware architecture, controls, and compliance framework. This standardized platform design means process parameters are maintained as you scale from 1/8" to 1" line sizes, significantly reducing tech transfer risk.
KlariFi™ is Repligen's intuitive, recipe-driven control platform. It features a point-and-click recipe editor, a flow-diagram interface mirroring the physical hardware, customizable data trending, Windows-integrated access control, and transferable recipes between systems.
The KrosFlo RS 50 TFF system supports flow rates of up to 83 liters per minute.
While highly dependent on your configuration the KrosFlo RS 10 TFF system typically supports minimum working volumes of 25mL or less dependent on bottle selection, membrane usage and process parameters.
Related Products
ProConnex® Flow Paths for Systems
Expertly designed plug-and-play flow path
ProConnex® Flow Paths for TFF and Chromatography processes are single-use and engineered for optimum performance, reproducibility and time savings. System specific flow paths provide speed and reliability. Configured flow paths prioritize process requirements and sterility.
TangenX® Flat Sheet Cassettes
First-to-market with single-use TFF flat sheet cassettes, TangenX® TFF technologies are reliable, scalable, and cost-effective solutions for efficient concentration and diafiltration.
Fluid Management
Repligen offers innovative, designed-for-purpose storage and transfer solutions for fluid management within and between unit operations. These include flow paths, tubing, valves and gaskets, bottles and containers, totes, carts and cleanroom process equipment.
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