Single-use TFF Systems for Rapid Bioprocessing

See how Repligen systems for tangential flow filtration combine unparalleled configurability with premium quality manufacturing. System designs maximize scalability from small to large volumes, flexibility between hollow fiber and flat sheet filter formats, and ability to use the same system in different unit operations while deploying ready-to-use application-specific flow path assemblies. 

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Configurable

Flexible across multiple parameters

KrosFlo® KTF Systems are configurable across multiple parameters, such as volume, application, and filter type. Multiple pumps of different sizes augment volume capability. Multiple pump types at different positions expand application and filter type support.

Volume Flexibility

  • Configure for a wide range of batch sizes
  • Fully compatible with ProConnex® TFF Flow Paths
  • Line 1 supports up to 2500 L (KrosFlo® Max X06 Filter)
  • Line 2 supports up to 1000 L (KrosFlo® K06 Filters)

Application Flexibility

  • Configure for multiple applications
  • iFIX software programmed for TFDF® applications
  • Fully compatible with ProConnex® TFDF® Flow Paths
  • Line 1 supports UF/DF  (1500 cm2)
  • Line 2 supports UF/DF (6000 cm2)
Configurable

Configure your KrosFlo® KTF System 

Experience the ease and simplicity of designing on-line.

  • Input requirements using pull down menus
  • Download on-line engineering drawings within a day
  • Receive as fast as 6 weeks ready-to-use

Configure your system

Configurable

Configurable ProConnex® Flow Paths

Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration.  Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.

ProConnex® Filter Assemblies

ProConnex® Configurator

In minutes, using an online drag and drop configurator and with customer input, Repligen representatives can configure a ProConnex® Flow Path specific to a process application in real-time.  The output of the ProConnex® Configurator are 3D engineering drawings and a preliminary quote, which greatly expedite the approval, production and delivery of the ProConnex® Flow Path.

  integrated

Integrated to support multiple scales and applications

The fully integrated KrosFlo® KTF Systems consist of hardware, software and flow path components. Key hardware components include a digital peristaltic pump, auxiliary pumps and an automatic back pressure valve. Different combinations  support multiple scales, unit operations and applications to maximize resource utilization.

Click on the tabs below to explore multiple systems to suit specific needs.

 

KrosFlo® KTF 200

KrosFlo® KTF 600

KrosFlo® KTF 1000

Typical process volumes Up to 200L Up to 1000L Up to 2500L
Max. filter area 1.0m2 (Hollow Fiber) 17.2m2 (Hollow Fiber) 17.2m2 (Hollow Fiber)
Configurable components 
Pump MagLev x 1 (i100) MagLev x 1 (600 Series) MagLev x 1 (2000 Series)
Flow rate Pump 1: 10 LPM Pump 1: 45 LPM Pump 1: 85 LPM
Max. pressure 1.5 Bar 2.0 Bar 2.5 Bar
Min. hold up volume 200 ml 2000 ml 3500 ml
Tubing Connections to Tank 3/8" ID 3/4" ID 1" and 3/4" ID
Hollow Fiber Filter N filter modules N, K, X filter modules X filter modules
Retentate Flow meter Clamp-on flow Ultrasonic

Feed Scale or Client Signal (4-20mA) Platform 220kg Platform 500kg, 1000kg, 2000kg Platform 2000kg+
Frame Width 24" 24" 24"
Modes of TFF Conc, Diaf, Final Conc, Fed Batch+

Flow-Path Sterile ProConnex® Flow Path

TMP Control Non-invasive value

Single-Use Pressure Sensor Sanitary PS 0.5", 1.0", 1.38"

Frame Stainless Steel 304, < 35Ra

Permeate Pump Tri lobe Peristaltic pump

Software and HMI      
HMI
 
Rockwell 6181P HMI/ Windows 10
PLC Allen Bradley Micrologix 1400

Software IFix 6.0
iHistorian
Dream Reports
E-signature
 







GMP Enables 21CFR11

Delta V Connectivity to Delta V

Permeate Flow Meter Clamp-on or Flow Thru Ultrasonic

 

KrosFlo® KTF 650

KrosFlo® KTF 700

KrosFlo® KTF 1600

KrosFlo® KTF 2000

Typical process volumes Up to 1000L Up to 1000L Up to 2500L Up to 5000L
Max. filter area 17.2m2 (Hollow Fiber) 17.2m2 (Hollow Fiber) 17.2m2 (Hollow Fiber) 17.2m2 (Hollow Fiber) x 2
Configurable components 
Pump MagLev x 2 MagLev x 2 MagLev x 2 MagLev x 2
Flow rate Pump 1: 4 LPM
Pump 2: 45 LPM
Pump 1: 10 LPM
Pump 2: 45 LPM
Pump 1: 45 LPM
Pump 2: 85 LPM
Pumps 1 and 2: 85 LPM
Max. pressure 1.5 Bar 2.0 Bar 2.5 Bar 2.5 Bar
Min. hold up volume 375 ml 750 ml 4500 ml 6000 ml
Tubing Connections to Tank 1/4" and 3/4" ID 3/8" and 3/4" ID 1" and 3/4" ID 1" ID
Hollow Fiber Filter T,S,N,K,X filter modules S,N,K X filter modules K,X filter modules X filter modules
Retentate Flow meter Clamp-on flow Ultrasonic

Feed Scale or Client Signal
(4-20mA)
Platform 220kg, 500kg, 2000kg Platform 200kg, 500kg, 2000kg Platform 1000kg+ Platform 2000kg+
Frame Width 30"
Modes of TFF Conc, Diaf, Final Conc, Fed Batch+

Flow-Path Sterile ProConnex® Flow Path

TMP Control Non-invasive value

Single-Use Pressure Sensor Sanitary PS 0.5", 1.0", 1.38"

Frame Stainless Steel 304, < 35Ra

Permeate Pump Tri lobe Peristaltic pump

Software and HMI
HMI Rockwell 6181P HMI/Windows 10
PLC Allen Bradley Micrologix 1400

Software IFix 6.0
iHistorian
Dream Reports
E-signature
 










GMP Enables 21CFR11

Delta V Connectivity to Delta V

Permeate Flow Meter Clamp-on or Flow Through Ultrasonic

   integrated

Proprietary software and closed flow path support compliance and quality

 

 

Base Control Platform iFIX 6.0 Software, 300 tags
Batch Reports Dream Report®
Security / Trends Windows® 10 with optional E-signature with iHistorian
PLC Allen Bradley® PLC Micrologix 1400
Communication Direct to iFIX:
  • OPC - DA, UA, AE
  • Ethernet IP - straight to PLC
Interface Options

Remote control via Ethernet IP
Connecting to other SCADA:

  • Delta V™, Emerson, Etc.
Regulatory 21CFR11 Enabled - Compliance and Documentation, including Audit Trail
Process Sequence Sequence programmed to Process Requirements
 
  READY TO SCALE FAST

Maintain processes while scaling to production

KrosFlo® KTF 200

KrosFlo® KTF 600

KrosFlo® KTF 2000

KrosFlo® KTF TFF Systems

KrosFlo KTF Systems include filters, ProConnex® Flow Path, pumps, process monitors, software and graphic display on a single skid, with a small footprint.

Click on the links below to explore different features.

The R Factor Video Blog
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Sweet hold-up volumes

You know that horrible feeling of watching precious material go to waste? Get rid of it. Configure a low hold-up volume KrosFlo® KTF System and ProConnex® Flow Path and recover more product. Every time.

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The art of TFF origami

The limitless design, exquisite accuracy and elegant simplicity of origami have been brought to TFF systems. Configure your optimal KTF KrosFlo® TFF system from the same starting point to satisfy your specific process needs. We have systems down to a science … and an art.

Optimized for use with Repligen Hollow Fiber Filter Modules

Below is a list of compatible Hollow Fiber Filters for use with KrosFlo® KTF Systems.

  Primary
Application
Volume Inlet/ Outlet Side
Port
Scale
Length
Effective Length (cm) Total
Length (cm)
Surface
area

MiniKros

               
MiniKros_2020.png Pilot-scale TFF 5 L - 50 L 1.5" TC 0.75" TC 1 x FL
2 x FL
3 x FL
20
41.5
65
25
46
70
0.16 - 0.31 m2
0.32 - 0.65 m2
0.51 - 1.0 m2

KrosFlo®

               
krosflo_2020.png Production-scale TFF 10 – 100L 3" TC 1.5" TC 1 x FL
2 x FL
3 x FL
20
41.5
65
23
46
69
0.78 - 1.6 m2
1.65 - 3.25 m2
2.55 - 5.10 m2

KrosFlo® MAX

               
krosflo_max_2020.png Production-scale TFF 100L – 1000+L 6" TC 1.5" TC R65
3 x FL
R85
49.9
67.9
107.5
73.5
91.5
131.1
4.30 - 10.40 m2
7.10 - 17.10 m2
11.30 - 11.35 m2

Manufacturing Centers of Excellence

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands,  Germany and Estonia.

Locations

Expert Project Management

Customer First.

Support is part of the Repligen DNA. Our goal is to provide exceptional customer experience, and to support the efficient and successful adoption and implementation of all Repligen products and services.

  • Field Application Support
  • Customer Service
  • Field Service Engineers

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