KrosFlo® KTF TFF Systems
Uniquely combining simplicity and configurability, Repligen KrosFlo® KTF Systems perform hollow fiber TFF to support multiple scales, unit operations, and solutions.
Single-use TFF Systems for Rapid Bioprocessing
See how Repligen systems for tangential flow filtration combine unparalleled configurability with premium quality manufacturing. System designs maximize scalability from small to large volumes, flexibility between hollow fiber and flat sheet filter formats, and ability to use the same system in different unit operations while deploying ready-to-use application-specific flow path assemblies.
Configurable
Flexible across multiple parameters
KrosFlo® KTF Systems are configurable across multiple parameters, such as volume, application, and filter type. Multiple pumps of different sizes augment volume capability. Multiple pump types at different positions expand application and filter type support.
Volume Flexibility
- Configure for a wide range of batch sizes
- Fully compatible with ProConnex® TFF Flow Paths
- Line 1 supports up to 2500 L (KrosFlo® Max X06 Filter)
- Line 2 supports up to 1000 L (KrosFlo® K06 Filters)
Application Flexibility
- Configure for multiple applications
- iFIX software programmed for TFDF® applications
- Fully compatible with ProConnex® TFDF® Flow Paths
- Line 1 supports UF/DF (1500 cm2)
- Line 2 supports UF/DF (6000 cm2)
Configurable
Configure your KrosFlo® KTF System
Experience the ease and simplicity of designing on-line.
- Input requirements using pull down menus
- Download on-line engineering drawings within a day
- Receive as fast as 6 weeks ready-to-use
Configurable
Configurable ProConnex® Flow Paths
Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration. Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.
ProConnex® Configurator
In minutes, using an online drag and drop configurator and with customer input, Repligen representatives can configure a ProConnex® Flow Path specific to a process application in real-time. The output of the ProConnex® Configurator are 3D engineering drawings and a preliminary quote, which greatly expedite the approval, production and delivery of the ProConnex® Flow Path.
integrated
Integrated to support multiple scales and applications
The fully integrated KrosFlo® KTF Systems consist of hardware, software and flow path components. Key hardware components include a digital peristaltic pump, auxiliary pumps and an automatic back pressure valve. Different combinations support multiple scales, unit operations and applications to maximize resource utilization.
Click on the tabs below to explore multiple systems to suit specific needs.
|
KrosFlo® KTF 200 |
KrosFlo® KTF 600 |
KrosFlo® KTF 1000 |
|
| Typical process volumes | Up to 200L | Up to 1000L | Up to 2500L |
| Max. filter area | 1.0m2 (Hollow Fiber) | 17.2m2 (Hollow Fiber) | 17.2m2 (Hollow Fiber) |
| Configurable components | |||
| Pump | MagLev x 1 (i100) | MagLev x 1 (600 Series) | MagLev x 1 (2000 Series) |
| Flow rate | Pump 1: 10 LPM | Pump 1: 45 LPM | Pump 1: 85 LPM |
| Max. pressure | 1.5 Bar | 2.0 Bar | 2.5 Bar |
| Min. hold up volume | 200 ml | 2000 ml | 3500 ml |
| Tubing Connections to Tank | 3/8" ID | 3/4" ID | 1" and 3/4" ID |
| Hollow Fiber Filter | N filter modules | N, K, X filter modules | X filter modules |
| Retentate Flow meter | Clamp-on flow Ultrasonic |
✓ |
✓ |
| Feed Scale or Client Signal (4-20mA) | Platform 220kg | Platform 500kg, 1000kg, 2000kg | Platform 2000kg+ |
| Frame Width | 24" | 24" | 24" |
| Modes of TFF | Conc, Diaf, Final Conc, Fed Batch+ |
✓ |
✓ |
| Flow-Path | Sterile ProConnex® Flow Path |
✓ |
✓ |
| TMP Control | Non-invasive value |
✓ |
✓ |
| Single-Use Pressure Sensor | Sanitary PS 0.5", 1.0", 1.38" |
✓ |
✓ |
| Frame | Stainless Steel 304, < 35Ra |
✓ |
✓ |
| Permeate Pump | Tri lobe Peristaltic pump |
✓ |
✓ |
| Software and HMI | |||
| HMI |
Rockwell 6181P HMI/ Windows 10 | ✓ | ✓ |
| PLC | Allen Bradley Micrologix 1400 |
✓ |
✓ |
| Software | IFix 6.0 iHistorian Dream Reports E-signature |
✓ |
✓ |
| GMP | Enables 21CFR11 |
✓ |
✓ |
| Delta V | Connectivity to Delta V |
✓ |
✓ |
| Permeate Flow Meter | Clamp-on or Flow Thru Ultrasonic |
✓ |
✓ |
|
|
KrosFlo® KTF 650 |
KrosFlo® KTF 700 |
KrosFlo® KTF 1600 |
KrosFlo® KTF 2000 |
| Typical process volumes | Up to 1000L | Up to 1000L | Up to 2500L | Up to 5000L |
| Max. filter area | 17.2m2 (Hollow Fiber) | 17.2m2 (Hollow Fiber) | 17.2m2 (Hollow Fiber) | 17.2m2 (Hollow Fiber) x 2 |
| Configurable components | ||||
| Pump | MagLev x 2 | MagLev x 2 | MagLev x 2 | MagLev x 2 |
| Flow rate | Pump 1: 4 LPM Pump 2: 45 LPM |
Pump 1: 10 LPM Pump 2: 45 LPM |
Pump 1: 45 LPM Pump 2: 85 LPM |
Pumps 1 and 2: 85 LPM |
| Max. pressure | 1.5 Bar | 2.0 Bar | 2.5 Bar | 2.5 Bar |
| Min. hold up volume | 375 ml | 750 ml | 4500 ml | 6000 ml |
| Tubing Connections to Tank | 1/4" and 3/4" ID | 3/8" and 3/4" ID | 1" and 3/4" ID | 1" ID |
| Hollow Fiber Filter | T,S,N,K,X filter modules | S,N,K X filter modules | K,X filter modules | X filter modules |
| Retentate Flow meter | Clamp-on flow Ultrasonic |
✓ |
✓ |
✓ |
| Feed Scale or Client Signal (4-20mA) |
Platform 220kg, 500kg, 2000kg | Platform 200kg, 500kg, 2000kg | Platform 1000kg+ | Platform 2000kg+ |
| Frame Width | 30" | ✓ | ✓ | ✓ |
| Modes of TFF | Conc, Diaf, Final Conc, Fed Batch+ |
✓ |
✓ |
✓ |
| Flow-Path | Sterile ProConnex® Flow Path |
✓ |
✓ |
✓ |
| TMP Control | Non-invasive value |
✓ |
✓ |
✓ |
| Single-Use Pressure Sensor | Sanitary PS 0.5", 1.0", 1.38" |
✓ |
✓ |
✓ |
| Frame | Stainless Steel 304, < 35Ra |
✓ |
✓ |
✓ |
| Permeate Pump | Tri lobe Peristaltic pump |
✓ |
✓ |
✓ |
| Software and HMI | ||||
| HMI | Rockwell 6181P HMI/Windows 10 | ✓ | ✓ | ✓ |
| PLC | Allen Bradley Micrologix 1400 |
✓ |
✓ |
✓ |
| Software | IFix 6.0 iHistorian Dream Reports E-signature |
✓ |
✓ |
✓ |
| GMP | Enables 21CFR11 |
✓ |
✓ |
✓ |
| Delta V | Connectivity to Delta V |
✓ |
✓ |
✓ |
| Permeate Flow Meter | Clamp-on or Flow Through Ultrasonic |
✓ |
✓ |
✓ |
integrated
Proprietary software and closed flow path support compliance and quality
|
|
|
|---|---|
| Base Control Platform | iFIX 6.0 Software, 300 tags |
| Batch Reports | Dream Report® |
| Security / Trends | Windows® 10 with optional E-signature with iHistorian |
| PLC | Allen Bradley® PLC Micrologix 1400 |
| Communication | Direct to iFIX:
|
| Interface Options |
Remote control via Ethernet IP
|
| Regulatory | 21CFR11 Enabled - Compliance and Documentation, including Audit Trail |
| Process Sequence | Sequence programmed to Process Requirements |
READY TO SCALE FAST
Maintain processes while scaling to production
KrosFlo® KTF 200
KrosFlo® KTF 600
KrosFlo® KTF 2000
KrosFlo® KTF TFF Systems
KrosFlo KTF Systems include filters, ProConnex® Flow Path, pumps, process monitors, software and graphic display on a single skid, with a small footprint.
Click on the links below to explore different features.
Solutions
You know that horrible feeling of watching precious material go to waste? Get rid of it. Configure a low hold-up volume KrosFlo® KTF System and ProConnex® Flow Path and recover more product. Every time.
2021-06-16
01:34
The limitless design, exquisite accuracy and elegant simplicity of origami have been brought to TFF systems. Configure your optimal KTF KrosFlo® TFF system from the same starting point to satisfy your specific process needs. We have systems down to a science … and an art.
2021-03-19
01:44
Optimized for use with Repligen Hollow Fiber Filter Modules
Below is a list of compatible Hollow Fiber Filters for use with KrosFlo® KTF Systems.
| Primary Application |
Volume | Inlet/ Outlet | Side Port |
Scale Length |
Effective Length (cm) | Total Length (cm) |
Surface area |
|
|---|---|---|---|---|---|---|---|---|
MiniKros |
||||||||
 |
Pilot-scale TFF | 5 L - 50 L | 1.5" TC | 0.75" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 46 70 |
0.16 - 0.31 m2 0.32 - 0.65 m2 0.51 - 1.0 m2 |
KrosFlo® |
||||||||
 |
Production-scale TFF | 10 – 100L | 3" TC | 1.5" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
23 46 69 |
0.78 - 1.6 m2 1.65 - 3.25 m2 2.55 - 5.10 m2 |
KrosFlo® MAX |
||||||||
 |
Production-scale TFF | 100L – 1000+L | 6" TC | 1.5" TC | R65 3 x FL R85 |
49.9 67.9 107.5 |
73.5 91.5 131.1 |
4.30 - 10.40 m2 7.10 - 17.10 m2 11.30 - 11.35 m2 |
Manufacturing Centers of Excellence
Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.
Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands, Germany and Estonia.
Expert Project Management
Customer First.
Support is part of the Repligen DNA. Our goal is to provide exceptional customer experience, and to support the efficient and successful adoption and implementation of all Repligen products and services.
- Field Application Support
- Customer Service
- Field Service Engineers
KrosFlo® KR2i TFF System
Single-use fully integrated benchtop TFF system for use in lab scale process development ranging from 2mL - 15L. Compatible with hollow fiber or flat sheet filters, this system offers many user-defined set-point controls so you can run complex applications in fully-automated modes.
ProConnex® Flow Paths for Systems
Expertly designed plug-and-play flow path
ProConnex® Flow Paths for TFF and Chromatography processes are single-use and engineered for optimum performance, reproducibility and time savings. System specific flow paths provide speed and reliability. Configured flow paths prioritize process requirements and sterility.
Spectrum® Hollow Fiber Filter Modules
Ideal for ultrafiltration, diafiltration and microfiltration, single-use hollow fiber filters for tangential flow filtration are packed in housings engineered to handle high pressures, with a choice of fiber chemistries and sizes from process development to manufacturing.
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