OPUS® RoboColumn® Pre-packed Columns
LEADER OF THE PACK
OPUS® RoboColumn® miniaturized pre-packed columns are on a 96-well plate, designed for automated chromatography process development and sample preparation using robotic liquid handling workstations.
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Click and quote OPUS® PD Pre-packed chromatography columns
Configure and quote pre-packed columns using the latest web-based technology.
When you are planning your next set of small-scale chromatography experiments, configure the columns online and obtain a quote in minutes. Simply login, select your format, volume and resin that meets your experimental design and add to cart. A quote with all details and pricing will be emailed to you within minutes.
Make your chromatography life easier.
FLEXIBILITY
Flexibility with pre-packed convenience
- Pre-packed with user-specified chromatography resin - over 300 resins
- Consistently packed with correct axial compression
- Operating linear flow-rate controlled by dispensing speed of the liquid handling workstation
- Demonstrated scale-up and scale-down due to constant residence time
- Large number of samples per time, ability to multiplex
Available formats
Internal Diameter |
Bed Height |
Column Volume |
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| cm | mm | cm | mm | mL | µL |
| 0.5 | 5.0 | 0.25 | 2.5 | 0.05 | 50 |
| 0.5 | 5.0 | 0.50 | 5.0 | 0.10 | 100 |
| 0.5 | 5.0 | 1.00 | 10.0 | 0.20 | 200 |
| 0.5 | 5.0 | 2.25 | 22.5 | 0.45 | 450 |
| 0.5 | 5.0 | 2.50 | 25.0 | 0.50 | 500 |
| 0.5 | 5.0 | 3.00 | 30.0 | 0.60 | 600 |
Minimum order is one row of 8 OPUS RoboColumn pre-packed columns with the same resin and column dimension.
Automation
Automated parallel chromatography
Shorten process development time significantly by performing parallel operation of OPUS RoboColumn pre-packed columns in robotic liquid handling workstations, implementing chromatography Design of Experiment (DOE) and QbD (Quality by Design).
Designed for robotic fluid handlers
Compatible with both Tecan and PerkinElmer platforms, the preferred supplier for OPUS RoboColumn automation is the Tecan Freedom EVO® robotic workstation.
- Simultaneous automated step-gradient elution into a 96-well plate
- Subsequent analysis in an integrated plate reader
- Flexible software wizard
The combination of OPUS RoboColumn and the Tecan Freedom EVO® robotic workstation delivers a complete integrated platform for state-of-the-art small-scale protein purification.
Rapid and simple AAV capture and polish DOE with new digital tools
This outline describes the advanced and fully automated purification development and optimization for an AAV vector with OPUS® RoboColumns® and Tecan’s Fluent™ robotic workstation.
High Performance
Process Development
- Resin screening / Condition screening
Development of a purification process for a specific molecule requires evaluation of the resin as well as operation conditions for each chromatography step. Using OPUS RoboColumn on a Tecan Freedom EVO® generated up to 96 pseudo-chromatograms (1536 data-points) in 1.5 days when comparing 12 CIEX resins at 8 different pH 3.7-7.0.
- 8 chromatographic separations in parallel with 16 fractions each in 16 minutes
- 96 individual buffers in 10 minutes
Sample Preparation / Process Analytical Technology (PAT)
- In-process fermentation monitoring
- Protein characterization
- Drug discovery
The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates. Non-purified samples require sample preparation before analysis can be executed. OPUS RoboColumn enables one-step purification of multiple samples in parallel.
Using the OPUS RoboColumn on a Tecan Freedom EVO® generates 2mg to 24mg API (Active Pharmaceutical Ingredient) per RoboColumn® run.
OPUS RoboColumn Pre-packed Chromatography Colums
Used in the industry since 2007, OPUS RoboColumn pre-packed columns are designed to work with robotic fluid handlers for fully automated parallel chromatography.
Up to 96 RoboColumn pre-packed columns (8 column types in parallel) can be individually arranged on a 96-well array plate according to application requirements. Resin is contained to form a bed between two filter frits and validated with consideration to resin compression requirements, to ensure high efficiency, good asymmetry and robust chromatography performance.
OPUS® RoboColumn®
internal design
OPUS® RoboColumn®
96-well plate
OPUS® RoboColumn®
5 mm ID x 30 mm H; V = 600 µL
OPUS® RoboColumn®
5 mm ID x 10 mm H; V = 200 µL
Manufacturing Centers of Excellence
Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.
Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands, Germany and Estonia.
Customer First.
Support is part of the Repligen DNA. Our goal is to provide exceptional customer experience, and to support the efficient and successful adoption and implementation of all Repligen products and services.
- Field Application Support
- Customer Service
- Field Service Engineers
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Resources
Specifications
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Plate size |
128.3 L x 86.0 W x 14.0 mm H |
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Plate material |
Polyoxymethylene copolymer |
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Number of wells |
96 |
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Column volume |
50 µL |
100 µL |
200 µL |
450 µL |
500 µL |
600 µL |
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Bed height |
2.5 mm |
5.0 mm |
10.0 mm |
22.5 mm |
25.0 mm |
30.0 mm |
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Column inner diameter |
5 mm |
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Column material |
Polypropylene |
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Chemical stability |
All commonly used aqueous buffers, pH 1 – 14, organic solvents*. Avoid the use of halogenated organic solvents, hexane |
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Column storage solution |
AIEC and CIEC Columns: 20 % ethanol + 150 mM sodium chloride |
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Recommended storage temperature |
AIEC, CIEC, HIC, SEC and MMC: +4°C to +30°C |
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Working temperature |
+4°C to +30°C |
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Maximum working pressure |
Up to 8.0 bar |
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Flow velocity |
16 – 1000 cm/h** |
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Labelling |
Laser-etched on every individual column body. Label contains:
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* Note: The chemical stability of the packed chromatography resin has also to be taken into consideration. For more information please refer to the manufacturer’s resin specification.