Modular, plug-and-play hardware with peristaltic pump 

The KrosFlo® KMPi TFF System achieves an unparalleled combination of integration, configurability and automation through modular, plug-and-play hardware.  All components, either directly or indirectly, connect to the main KrosFlo® KMPi peristaltic pump, which then communicates with the KF Comm 2 software for control and monitoring.

  • Configure a minimal number of components for simple applications such as concentration (C) or diafiltration (D)
  • Plug-and-play additional components for fractionation (F) and multi-step processes (i.e. CDC/D/C)

Click on the tabs below to explore different combinations of concentration and diafiltration configurations using the KrosFlo® KMPi System.

Concentrate with a streamlined configuration

C = Concentration D = Diafiltration F = Feed

Add a scale and auxiliary pump to access diafiltration applications

C = Concentration D = Diafiltration F = Feed

Build process understanding with increased sensors

C = Concentration D = Diafiltration F = Feed


ProConnex® Flow Paths for KMPi

Save time, streamline operations and hit the ground running​

These ready-to-go flow paths create time and quality gains for every run. Enjoy the affordable convenience of off-the-shelf flow paths designed by the engineers who created the system you adore. These are created specifically to run Spectrum® Hollow Fiber or TangenX® flat sheet cassettes on the KrosFlo KMPi systems. Filter sold separately.

ProConnex® Filter Assemblies


Transfer bi-directional data through
proprietary board

An advanced microprocessor-based controller is integrated into the main KrosFlo® KMPi System pumps, enabling plug-and-play hardware component behavior. Bi-directional communication is established between the KF Comm 2 software and hardware components, such as the Automatic Back Pressure Control Valve, scales, auxiliary pumps and the Konduit device.

KrosFlo® KR2i Virtual Demo Series

In this 6-part series, Julie Grace, and Carl Breuning, provide viewers with a virtual training on the KrosFlo® KR2i TFF system. This training series will cover the following topics:

  • Introduction to system components
  • Installation of flow-path and filter
  • KF Comm2 software overview
  • Recipe creation
  • Running a CFC/D/C experiment
  • Recovery of product


Menu-driven Software for Optimized TFF

KF Comm 2 Software, backward compatible with legacy KrosFlo® KR2i and KrosFlo® KMPi Systems, is conveniently installed on any computer using the Windows Operating System. The software is compatible with Chrome, Mozilla, Firefox and Microsoft Explorer browsers and is always current with automatic software and firmware updates. 

  • Proprietary software optimized for KrosFlo® TFF Systems
  • Validated for GMP Processes with optional 21 CFR Part 11 option
  • Intuitive workflow with 13 modes of operation



Automatic Back Pressure Control Valve

A Repligen-exclusive technology, the Automatic Back Pressure Control Valve adds transmembrane pressure (TMP) control to every TFF process, reducing operator hands-on-time and improving process reproducibility. 

  • Automatically adjust tubing pinch distance according to user-defined set-points
  • Maintain uniform TMP throughout process run, decreasing membrane fouling
  • Control permeate pressure and flow during microfiltration applications
  • Use with compatible tubing sizes 13, 14, 16, 25, HP15, 17, HP24, 18, HP35, 36, and HP36
  • Activate automatically using inlet, retentate, permeate or transmembrane pressures 

Monitor and evaluate conductivity, UV and temperature 

Konduit is an add-on module ready to use with the KrosFlo® KR2i and KMPi TFF Systems, with the ability to scale from 2mL to 500L process volumes.

Conductivity Range 0.1 to 100 mS/cm
Temperature Range0-70°
Pressure Range up to 75 psi (5 bar)
Dimensions(L x W x H): 7¾" x 4¾" x 4½"
Weight 1.6 kg


Ideal for Gene Therapy from Lab to Manufacturing

Run TFF development experiments and compliant small-scale manufacturing runs on a single platform. Start with the KrosFlo® KR2i System with  volumes as low as 10 mL. Scale up to 500 L with the KrosFlo® KMPi System while maintaining pump design, system architecture and software.

  • Scale-up and scale-down with a single platform
  • Configurable across applications (C, D and CFC/D/C)
  • Modern 21 CFR Part 11 compliance software built on secure Microsoft® SQL Server 

Scalable From Lab-scale to Process-scale

The KrosFlo® family of TFF Systems for concentration and diafiltration supports scale up from process development to commercial manufacturing.

KrosFlo® KR2i
2 ml - 15 L

KrosFlo® KMPi
100 mL - 500 L

KrosFlo® KTF
10 L - 2000+ L

KrosFlo® KMPi TFF System

The fully integrated KrosFlo® KMPi TFF System consists of hardware, software and flow path components. Key hardware components include: a digital peristaltic pump, auxiliary pumps and an automatic back pressure valve.

The KF Comm 2 software features a multi-language interface for complete monitoring and control and an optional 21 CFR Part 11 environment. Add tubing and sanitize/sterilize your own flow path around a Spectrum® hollow fiber filter or build a closed and gamma-irradiated ProConnex® Flow Path from over 250 components.

Click on the links below to explore different features.


Optimized for use with Repligen Hollow Fiber Filter Modules

Volume Inlet/ Outlet Side
Effective Length (cm) Total
Length (cm)

MiniKros Sampler

MiniKros-Sampler.png Batch TFF of small volumes 3 L -15 L 0.75" TC 0.75" TC 1 x FL
2 x FL
3 x FL
490 – 1000 cm2
1000 - 2200 cm2
1600 - 3400 cm2


MiniKros.png Pilot-scale TFF 5 L - 50 L 1.5" TC 0.75" TC 1 x FL
2 x FL
3 x FL
0.16 - 0.31 m2
0.32 - 0.65 m2
0.51 - 1.0 m2


KrosFlo.png Production-scale TFF 10 – 100L 3" TC 1.5" TC 1 x FL
2 x FL
3 x FL
0.78 - 1.6 m2
1.65 - 3.25 m2
2.55 - 5.10 m2

Components and Accessories

  • IP 33 Enclosure
  • 600 RPM pump with reversible drive
  • 90-260 Vrms @ 50/60 Hz
  • Standard Easy Load 3 Pump Head for precision tubing L/S number 13, 14, 16, 25, 17, and 18 provided.
  • (Optional) Easy Load 3 pump head for high performance tubing L/S numbers 15, 24, 35, 36
  • ETL listed and CE/UL/cUL marked
  • Pump Drive Dimensions: 10.5 in x 8 in x 8 in
Konduit adds:
  • End point conductivity control for UF /DF
  • End point UV control for diafiltering free drug from drug delivery particles
  • End point UV control for concentration of certain molecules
  • UV alarm for membrane breakthrough
  • A260 and A280 light sources available

*UV components sold separately


  • Measures and displays 4 pressures: Feed Pressure, Retentate Pressure, Permeate Pressure, Transmembrane Pressure (TMP)
  • User-defined pressure alarms and stop set-points
  • Available with different connector types (Luer and TC)  and selected materials of construction (PS, PC)

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Maintain user-defined feed weight set-points, and total permeate mass during TFF processes.

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KrosFlo® KR Jr Auxiliary Pump

Auxiliary pumps automatically increase or decrease buffer/product addition to feed reservoir.

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Manufacturing Centers of Excellence

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands,  Germany and Estonia.


Expert Project Management

Customer First.

Support is part of the Repligen DNA. Our goal is to provide exceptional customer experience, and to support the efficient and successful adoption and implementation of all Repligen products and services.

  • Field Application Support
  • Customer Service
  • Field Service Engineers

Have a question?




Process volume Up to 500L
Filter surface area Up to 5.1 m2
Main drive/Feed pump Up to 11LPM (650 RPM)
Pressure transducers Up to 3 Leur-style, polysulfone single-use (-0.48 to 5.2bar)
Scales 60Kg (included); Optional: 60Kg, 200Kg, 500Kg and 1000Kg
Auxiliary pumps KrosFlo® Research I, 600 RPM
Tubing PharmaPure, TPE, size #26, #73 and #82
TMP control Automatic backpressure control valve (Non-invasive/No-product contact)
Reservoirs 10L polypropylene with 4-port cap and drain
Tablet as HMI Microsfot Surface Pro
Software KFComm 2 with optional 21CFR part 11 package
Compliance UL and CE certified