XCell® ATF Devices and Controllers

XCell^® ATF Systems simplify and fast-track upstream intensification by providing an integrated solution consisting of an XCell^® ATF Device, XCell® ATF Controller, software, tubing, pressure sensors and accessories. The family of Systems supports diverse applications across development from 1L to 5000 L.

XCell® ATF Device Size Selection Guide

Presented are hollow fiber filter areas, XCell^® ATF Pump volumes, and typical bioreactor working volumes paired with each XCell ATF Device. Actual capacity and vessel size depend upon specific cell culture process conditions.

Device	Effective filter surface area (m2)	Approximate ATF volume exchanged (L)	Suspension cultures volume (L)
XCell ATF 1	0.022	0.017	0.5 - 2
XCell ATF 2	0.13	0.1	2 - 10
XCell ATF 4	0.77	0.4	10 - 50
XCell ATF 6	2.5	1.3	50 - 200
XCell ATF 10	11.0	6.0	200 - 1000

XCell^® ATF Single-use Devices

XCell ATF 1 Single-use Device

XCell ATF 2 Single-use Device

XCell ATF 6 Single-use Device

XCell ATF10 Single-use Device

XCell ATF Single-use Tubing Sets

Filter Wetting Procedure

XCell ATF Single-use Devices are supplied dry. To ensure robust filter performance, it is important to wet the filter prior to use. The XCell ATF Tubing Set and the suggested wetting protocols are designed to:

Ensure efficient and complete filter wetting by minimizing the formation of air pockets during the wetting process
Enable pre-use filter integrity testing while maintaining sterility
Provide a means for evaluating the sterility of the XCell ATF device prior to establishing the bioreactor connection
Allow change-out of the XCell ATF device during a cell culture run without interrupting the bioreactor or XCell ATF operation

XCell ATF Stainless Steel Devices

XCell ATF 2 Stainless Steel Device

XCell ATF 4 Stainless Steel Device

XCell ATF 6 Stainless Steel Device

XCell ATF 10 Stainless Steel Device

How to Install a Hollow Fiber Filter

Watch the video on how to install a hollow fiber filter into an XCell ATF 10 stainless steel device.

XCell^® Controllers

XCell Lab Controller

for use with:
XCell ATF 1 (SU)
XCell ATF 2 (SU,SS)
XCell ATF 4 (SS)

XCell LS Controller

for use with:
XCell ATF 4 (SS)
XCell ATF 6 (SU, SS)
XCell ATF 10 (SU, SS)

XCell C410 Controller

for use with:
XCell ATF 4 (SS)
XCell ATF 6 (SU, SS)
XCell ATF 10 (SU, SS)

XCell C410 Controller Cart
(optional)

Advancing Upstream Intensification Through Partnership

Repligen has partnered with Sartorius to integrate the XCell^® ATF large scale controller technology into the Biostat 3G STR^® bioreactor to advance Upstream Intensification for biopharmaceutical manufacturers.

XCell ATF devices by scale


	XCell^® ATF 1	XCell^® ATF 2	XCell^® ATF 4
Typical Bioreactor size (L)	0.5 - 2	2 - 10	10 - 50
Format	SU	SU, SS	SS
Chemistry	PES	PES, PS	PES, PS
Typical pore size SU	0.2µ	0.2µ	0.2µ
Typical pore size SS	NA	0.2µ, 0.5µ, 0.50Da	0.2µ, 0.5µ, 0.50KDa
Effective surface area (m²)	0.022	0.13	0.77
Filter height (cm)	60	60	30
Displacement volume (L)	0.017	0.1	0.4
ATF flow range (Lpm)	0.008 - 0.140	0.3 - 1.5	3 - 8
Typical scalable flow/fiber and filtrate rate	12 mL/min/fiber and 4-6 LMH
Controller	XCell Lab Scale Controller		XCell Lab Scale Controller XCell C410 Controller XCell LS Controller


	XCell^® ATF 4	XCell^® ATF 6
Typical Bioreactor size (L)	10 - 50	50 - 200
Format	SS	SU, SS
Chemistry	PES, PS	PES, PS
Typical pore size SU	0.2µ	0.2µ
Typical pore size SS	0.2µ, 0.5µ, 0.50KDa	0.2µ, 0.5µ, 0.50KDa
Effective surface area (m²)	0.77	2.5
Filter height (cm)	30	60
Displacement volume (L)	0.4	1.3
ATF flow range (Lpm)	3 - 8	10 - 20
Typical scalable flow/fiber and filtrate rate
Controller	XCell Lab Scale Controller XCell C410 Controller XCell LS Controller	XCell C410 Controller XCell LS Controller


	XCell^® ATF 6	XCell^® ATF 10
Typical Bioreactor size (L)	50 - 200	200 - 1000
Format	SU, SS	SU, SS
Chemistry	PES, PS	PES, PS
Typical pore size SU	0.2µ	0.2µ
Typical pore size SS	0.2µ, 0.5µ, 0.50KDa	0.2µ, 0.5µ, 0.50KDa
Effective surface area (m²)	2.5	11
Filter height (cm)	60	60
Displacement volume (L)	1.3	6
ATF flow range (Lpm)	10 - 20	30 - 80
Typical scalable flow/fiber and filtrate rate	12 mL/min/fiber and 4-6 LMH	12 mL/min/fiber and 4-6 LMH
Controller	XCell C410 Controller XCell LS Controller	XCell C410 Controller XCell LS Controller