Intensified Production of Recombinant Vesicular Stomatitis Virus-Based Vectors by Tangential Flow Depth Filtration

In this study, we evaluated the use of a tangential flow depth filtration (TFDF) unit for both perfusion cultivation and continuous harvesting with clarification in a single unit operation at the 3 L scale. The intensified production of two different rVSV-based vectors, one inducing classical cytopathic effect and one mediating cell fusion reactions, was compared to optimized batch control processes.

 

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Game-changing TFDF® (Tangential Flow Depth Filtration) technology that simplify and intensify upstream viral vector bioprocessing, ideal for AAV, lentivirus, oncolytic, retrovirus, adenovirus, exosomes, and cells on microcarriers.