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Screening and Sample Preparation
OPUS® MiniChrom® Pre-packed Columns

 

OPUS® MiniChrom® Columns are designed for bench-scale resin screening, process development and sample preparation.

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Shop opus® Minichrom® Columns for AAV

Pre-packed Columns for process development

Designed for bench-scale resin screening, process development,  and sample preparation, OPUS® MiniChrom® Columns are pre-packed with user-specified resin and supplied ready to use with any liquid chromatography system.  Flexible column dimension configurations (internal diameters, bed heights, volumes) allow comparative evaluation of process parameters and the construction of customized resin libraries.

OPUS® MiniChrom® Pre-packed Columns

Currently platformed in various process development projects, as well as for resin sampling by various resin vendors, over 30,000 OPUS® MiniChrom® Columns have been in used in the industry since 2007.  Individual laser labeling containing Part Number and Serial Number ensure traceability to the corresponding performance data. 

  • Pre-packed with user-specified chromatography resin - over 200 resins
  • 8 different column dimensions
  • Defined, documented packing procedures with correct compression factor to deliver high performance from process resins ​​​​
    • Reduced HETP consistent near 5
    • Asymmetry stable at 1.1 to 1.2
    • Up to 30 bar pressure resistance
  • Compatible with all common liquid chromatography systems - 1/16" female connectors
  • A Certificate of Analysis (CoA) for each individual column is available on request

See resin list

Available formats

     

Internal
Diameter

 Bed
Height		 Column Volume
cm mm cm   mm mL  
0.5 5.0 1.0 10.0 0.2  
0.5 5.0 2.5 25.0 0.5  
0.5 5.0 5.0 50.0 1.0  
0.8 8.0 2.0 20.0 1.0  
0.8 8.0 5.0 50.0 2.5  
0.8 8.0 10.0 100.0 5.0  
1.13 11.3 5.0 50.0 5.0  
1.13 11.3 10.0 100.0 10.0  
OPUS MiniChrom Columns

Internal diameter x Bed Height (cm)

Convenient

Pre-packed columns provided ready to use

Flexible

Packed with any user-specified process resin; Compatible with any liquid chromatography system

Variable configurations

Internal diameter, bed height, and column volume configurations enable customization to specific process development needs

High reproducibility

Reliable performance from process resins batch to batch

Broad scalability

Up to 10 ml volume and 3 diameters

Cost-effective

Small configuration drives reduced material needs, and saves valuable resin samples while delivering similar performance to larger preparative or process columns

 

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

about Repligen Manufacturing

proven Applications

​Process Development

  • Resin screening / Condition screening
  • Parameter optimization

The development of a purification workflow for a specific molecule requires evaluation of the resin and the operating conditions for each chromatography step.

Using a scale-down chromatography column configuration drives efficiency and higher throughput. The performance of OPUS® MiniChrom® Columns is equivalent to the performance of larger columns, yielding reliable and predictive chromatography performance data. Using these smaller scale-down columns for process development saves valuable samples, cost, resources and time.

Results can be saved in a resin library of preferred resins.

Scharl et al., Trend analysis of performance parameters of pre-packed columns for protein chromatography over a time span of ten years,  Journal of Chromatography A, 1465 (2016) 63–70.

Sample preparation / Process Analytical Technology (PAT)

  • Larger protein quantities

The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates.  Non-purified samples require sample preparation before analysis can be executed. OPUS® MiniChrom® Columns enable one-step purification of multiple samples in parallel.

Use of OPUS® MiniChrom® Columns generates 8-400 mg of sample per run.

OPUS MiniChrom Columns

Specifications

Column inner diameter

5 mm

    

8 mm

     11.3 mm  

Packed bed height

10 mm

 25 mm

50 mm

20 mm

50 mm

100 mm

50 mm

 100 mm

Column volume

0.2 mL

0.5 mL

1.0 mL

1.0 mL

2.5 mL

5.0 mL

5.0 mL

 10.0 mL

Inner cross-sectional area

0.2 cm2

    

0.5 cm2

    

1.0 cm2

  

Connectors

Fingertight 1/16", female (10-32)

Column material

Polypropylene

Chemical stability

All commonly used aqueous buffers, pH 1 – 14, organic solvents*.
Avoid the use of halogenated organic solvents, hexane

Column storage solution

AIEC and CIEC Columns: 20 % ethanol + 150 mM sodium chloride
HIC, SEC, Affinity and MMC Columns: 20 % ethanol
CHT, CFT Columns (Bio-Rad): 20 % ethanol + 10 mM sodium phosphate, pH 6.8
ProSep® Columns(Millipore):1 % benzyl alcohol + 0.1 M sodium acetate, pH 5.1

Recommended storage temperature

AIEC, CIEC, HIC, SEC and MMC: +4°C to +30°C
Affinity Columns: +2°C to +8°C

Working temperature

+4°C to +30°C

Maximum working pressure

Up to 30 bar

Flow velocity

16 – 1000 cm/h**

Labelling

Laser-etched on every individual column body. Label contains:

  • Product number
  • Serial number
  • Resin name
  • Column dimensions (ID x H, V)
  • Flow direction

* The chemical stability of the packed chromatography resin also has also to be taken into consideration. For more information, please refer to the manufacturer’s resin specification.

Resources

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