OPUS RoboColumn :: Repligen

OPUS^®RoboColumns^® are miniaturized pre-packed columns on a 96-well plate, designed for automated chromatography process development and sample preparation using robotic liquid handling workstations.

Automated parallel Chromatography

Shorten process development time significantly by performing parallel operation of OPUS^® RoboColumns^® in robotic liquid handling workstations, implementing chromatography Design of Experiment (DOE) and QbD (Quality by Design).

OPUS^® RoboColumn^®Pre-packed Columns

Used in the industry since 2007, OPUS^® RoboColumns^® are designed to work with robotic fluid handlers for fully automated parallel chromatography.

Up to 96 RoboColumns^® (8 column types in parallel) can be individually arranged on a 96-well array plate according to application requirements. Resin is contained to form a bed between two filter frits and validated with consideration to resin compression requirements, to ensure high efficiency, good asymmetry and robust chromatography performance.

Pre-packed with user-specified chromatography resin - over 300 resins
Consistently packed with correct axial compression
Operating linear flow-rate controlled by dispensing speed of the liquid handling workstation
Demonstrated scale-up and scale-down due to constant residence time
Large number of samples per time, ability to multiplex

See resin list

Available formats


Internal Diameter		Bed Height		Column Volume
cm	mm	cm	mm	mL	µL
0.5	5.0	0.25	2.5	0.05	50
0.5	5.0	0.50	5.0	0.10	100
0.5	5.0	1.00	10.0	0.20	200
0.5	5.0	2.25	22.5	0.45	450
0.5	5.0	2.50	25.0	0.50	500
0.5	5.0	3.00	30.0	0.60	600

Minimum order is one row of 8 OPUS^® RoboColumns^® with the same resin and column dimension.

OPUS^® RoboColumn^®
internal design

OPUS^® RoboColumn^®
96-well plate

OPUS^® RoboColumn^®
5 mm ID x 30 mm H; V = 600 µL

OPUS^® RoboColumn^®
5 mm ID x 10 mm H; V = 200 µL

Convenient

Pre-packed columns provided ready to use

Flexible

Packed with user-specified chromatography resin, multiple column volumes

Automated

Use with Tecan and Perkin Elmer robotic fluid handlers

Fast

Process up to 8 OPUS^® RoboColumns^® in parallel, shorten development time

Cost-effective

Reduces resource requirements

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

about Repligen Manufacturing

Designed for robotic Fluid Handlers

Compatible with both Tecan and PerkinElmer platforms, the preferred supplier for OPUS^®RoboColumn^® automation is the Tecan Freedom EVO^® robotic workstation.

Simultaneous automated step-gradient elution into a 96-well plate
Subsequent analysis in an integrated plate reader
Flexible software wizard

The combination of OPUS^® RoboColumns^®and the Tecan Freedom EVO^® robotic workstation delivers a complete integrated platform for state-of-the-art small-scale protein purification.

screening and sample preparation Applications

Process Development

Resin screening / Condition screening

Development of a purification process for a specific molecule requires evaluation of the resin as well as operation conditions for each chromatography step. Using OPUS^® RoboColumn^® on a Tecan Freedom EVO^® generated up to 96 pseudo-chromatograms (1536 data-points) in 1.5 days when comparing 12 CIEX resins at 8 different pH 3.7-7.0.

8 chromatographic separations in parallel with 16 fractions each in 16 minutes
96 individual buffers in 10 minutes

Sample Preparation / Process Analytical Technology (PAT)

In-process fermentation monitoring
Protein characterization
Drug discovery

The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates. Non-purified samples require sample preparation before analysis can be executed. OPUS^® RoboColumns^®enable one-step purification of multiple samples in parallel.

Using the OPUS^® RoboColumn^® on a Tecan Freedom EVO^® generates 2mg to 24mg API (Active Pharmaceutical Ingredient) per RoboColumn^®run.

Specifications

Plate size	128.3 L x 86.0 W x 14.0 mm H
Plate material	Polyoxymethylene copolymer
Number of wells	96
Column volume	50 µL	100 µL	200 µL	450 µL	500 µL	600 µL
Bed height	2.5 mm	5.0 mm	10.0 mm	22.5 mm	25.0 mm	30.0 mm
Column inner diameter	5 mm
Column material	Polypropylene
Chemical stability	All commonly used aqueous buffers, pH 1 – 14, organic solvents*. Avoid the use of halogenated organic solvents, hexane
Column storage solution	AIEC and CIEC Columns: 20 % ethanol + 150 mM sodium chloride HIC, SEC, Affinity and MMC Columns: 20 % ethanol CHT, CFT Columns (Bio-Rad): 20 % ethanol + 10 mM sodium phosphate, pH 6.8 ProSep^® Columns(Millipore):1 % benzyl alcohol + 0.1 M sodium acetate, pH 5.1
Recommended storage temperature	AIEC, CIEC, HIC, SEC and MMC: +4°C to +30°C Affinity Columns: +2°C to +8°C
Working temperature	+4°C to +30°C
Maximum working pressure	Up to 8.0 bar
Flow velocity	16 – 1000 cm/h**
Labelling	Laser-etched on every individual column body. Label contains: Product number Resin name Column volume in µL

* Note: The chemical stability of the packed chromatography resin has also to be taken into consideration. For more information please refer to the manufacturer’s resin specification.

** The Liquid Transfer menu of the Te‑Chrom™ Wizard enables set up of the flow rate for each individual dispensing and chromatography step (5.1.1.6 Liquid Transfer, Te‑Chrom™ Wizard Software Manual, 396076, en, V1.0).

Resources

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OPUS® RoboColumns® are miniaturized pre-packed columns on a 96-well plate, designed for automated chromatography process development and sample preparation using robotic liquid handling workstations.