Screening and Sample Preparation
OPUS® RoboColumns® are miniaturized pre-packed columns on a 96-well plate, designed for automated chromatography process development and sample preparation using robotic liquid handling workstations.
Automated parallel Chromatography
Shorten process development time significantly by performing parallel operation of OPUS® RoboColumns® in robotic liquid handling workstations, implementing chromatography Design of Experiment (DOE) and QbD (Quality by Design).
OPUS® RoboColumn® Pre-packed Columns
Used in the industry since 2007, OPUS® RoboColumns® are designed to work with robotic fluid handlers for fully automated parallel chromatography.
Up to 96 RoboColumns® (8 column types in parallel) can be individually arranged on a 96-well array plate according to application requirements. Resin is contained to form a bed between two filter frits and validated with consideration to resin compression requirements, to ensure high efficiency, good asymmetry and robust chromatography performance.
- Pre-packed with user-specified chromatography resin - over 300 resins
- Consistently packed with correct axial compression
- Operating linear flow-rate controlled by dispensing speed of the liquid handling workstation
- Demonstrated scale-up and scale-down due to constant residence time
- Large number of samples per time, ability to multiplex
Available formats
| Internal Diameter | Bed Height |
Column Volume |
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| cm | mm | cm | mm | mL | µL |
| 0.5 | 5.0 | 0.25 | 2.5 | 0.05 | 50 |
| 0.5 | 5.0 | 0.50 | 5.0 | 0.10 | 100 |
| 0.5 | 5.0 | 1.00 | 10.0 | 0.20 | 200 |
| 0.5 | 5.0 | 2.25 | 22.5 | 0.45 | 450 |
| 0.5 | 5.0 | 2.50 | 25.0 | 0.50 | 500 |
| 0.5 | 5.0 | 3.00 | 30.0 | 0.60 | 600 |
Minimum order is one row of 8 OPUS® RoboColumns® with the same resin and column dimension.
OPUS® RoboColumn®
internal design
OPUS® RoboColumn®
96-well plate
OPUS® RoboColumn®
5 mm ID x 30 mm H; V = 600 µL
OPUS® RoboColumn®
5 mm ID x 10 mm H; V = 200 µL
Convenient
Pre-packed columns provided ready to use
Flexible
Packed with user-specified chromatography resin, multiple column volumes
Automated
Use with Tecan and Perkin Elmer robotic fluid handlers
Fast
Process up to 8 OPUS® RoboColumns® in parallel, shorten development time
Cost-effective
Reduces resource requirements
Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.
Designed for robotic Fluid Handlers
Compatible with both Tecan and PerkinElmer platforms, the preferred supplier for OPUS®RoboColumn® automation is the Tecan Freedom EVO® robotic workstation.
- Simultaneous automated step-gradient elution into a 96-well plate
- Subsequent analysis in an integrated plate reader
- Flexible software wizard
The combination of OPUS® RoboColumns® and the Tecan Freedom EVO® robotic workstation delivers a complete integrated platform for state-of-the-art small-scale protein purification.
screening and sample preparation Applications
Process Development
- Resin screening / Condition screening
Development of a purification process for a specific molecule requires evaluation of the resin as well as operation conditions for each chromatography step. Using OPUS® RoboColumn® on a Tecan Freedom EVO® generated up to 96 pseudo-chromatograms (1536 data-points) in 1.5 days when comparing 12 CIEX resins at 8 different pH 3.7-7.0.
- 8 chromatographic separations in parallel with 16 fractions each in 16 minutes
- 96 individual buffers in 10 minutes
Sample Preparation / Process Analytical Technology (PAT)
- In-process fermentation monitoring
- Protein characterization
- Drug discovery
The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates. Non-purified samples require sample preparation before analysis can be executed. OPUS® RoboColumns® enable one-step purification of multiple samples in parallel.
Using the OPUS® RoboColumn® on a Tecan Freedom EVO® generates 2mg to 24mg API (Active Pharmaceutical Ingredient) per RoboColumn® run.
Specifications
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Plate size |
128.3 L x 86.0 W x 14.0 mm H |
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Plate material |
Polyoxymethylene copolymer |
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Number of wells |
96 |
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Column volume |
50 µL |
100 µL |
200 µL |
450 µL |
500 µL |
600 µL |
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Bed height |
2.5 mm |
5.0 mm |
10.0 mm |
22.5 mm |
25.0 mm |
30.0 mm |
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Column inner diameter |
5 mm |
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Column material |
Polypropylene |
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Chemical stability |
All commonly used aqueous buffers, pH 1 – 14, organic solvents*. Avoid the use of halogenated organic solvents, hexane |
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Column storage solution |
AIEC and CIEC Columns: 20 % ethanol + 150 mM sodium chloride |
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Recommended storage temperature |
AIEC, CIEC, HIC, SEC and MMC: +4°C to +30°C |
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Working temperature |
+4°C to +30°C |
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Maximum working pressure |
Up to 8.0 bar |
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Flow velocity |
16 – 1000 cm/h** |
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Labelling |
Laser-etched on every individual column body. Label contains:
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* Note: The chemical stability of the packed chromatography resin has also to be taken into consideration. For more information please refer to the manufacturer’s resin specification.
** The Liquid Transfer menu of the Te‑Chrom™ Wizard enables set up of the flow rate for each individual dispensing and chromatography step (5.1.1.6 Liquid Transfer, Te‑Chrom™ Wizard Software Manual, 396076, en, V1.0).