Using award-winning alternating tangential flow (ATF) technology, XCell ATF® cell retention devices deliver high cell concentration and process intensification during cell culture.
How ATF works
The XCell ATF® System is based on award-winning alternating tangential flow (ATF) technology, created by the action of a diaphragm moving upward and downward within a pump head, connected to a filter housing and attached to a bioreactor. Alternating tangential flow is attained by the action of a diaphragm pump.
100% cell retention, 80% faster implementation, no autoclave
The XCell ATF® Single-use System comes in multiple sizes from lab-scale to production-scale. Each system consists of a single-use device, a single-use hollow fiber filter cartridge, and a control system.
100% cell retention, high cell densities and product yields
The XCell ATF® System in stainless steel comes in multiple sizes from lab-scale to production-scale. Each system consists of a diaphragm pump in a stainless steel housing, a single-use hollow fiber filter cartridge, and a control system.
Enables continuous processing, provides clarified harvest ready for capture chromatography
100% cell retention, high cell concentration and viability; connects to any bioreactor and can be used in a variety of applications
Gentle action reduces cell stress, increases cell viability and productivity; Self-cleaning, reduces biofilm build up on the hollow fiber filter
Reduces implementation time by up to 80%, no autoclave
From process development-scale to production-scale
Field Applications and Service Specialists provide worldwide support
Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.
Perfusion, or upstream continuous processing, has been used since the 1980s. High cell density perfusion with XCell ATF® devices delivers higher cell concentration and cell viability.
N-1 perfusion refers to the intensification of cell growth in the N-1 bioreactor prior to the production N bioreactor, whh remains in fed-batch mode. This process enables high cell density seeding of the production bioreactor.
Fed-Batch:
High Productivity Harvest
High Productivity Harvest (HPH) is a novel application developed by Repligen that enables you to harvest a fed-batch bioreactor in a fully sterile manner while eliminating both centrifugation and depth filtration.
High Productivity Harvest: A Novel Approach to Fed-batch Process Intensification
Mitigation of Protein Retention in Perfusion Process and Effect of Filter Pore Size
Demonstration of XCell ATF® 2 and XCell ATF® 6 Single-use Performance
Mitigation of Protein Retention in High Density Perfusion Process
Tools for Intensification of Cell Culture Production and Continuous Downstream Processing
ATF Perfusion Technology: Improved Fed-Batch Throughput and Reduced Seed Train Expansion
Special Report on Continuous Bioprocessing: Upstream, Downstream, Ready for Prime Time?
WuXi Biologics Expands Perfusion Cell Culture Capabilities using ATF 10 System
Continuous Manufacturing: A Changing Processing Paradigm
Developing an upstream process for a monoclonal antibody including medium optimization
Frequently asked questions | perfusion