You are using an unsupported browser.
Some features of this site may not function properly. For optimal user experience, please view this site in Chrome, Firefox, Safari, or Edge.

XCell ATF®

Simplify and intensify upstream bioprocessing. The XCell ATF® System, in single-use or stainless steel format, delivers high cell retention. 

Simplify and intensify cell culture

Using award-winning alternating tangential flow (ATF) technology, XCell ATF® cell retention devices deliver high cell concentration and process intensification during cell culture. 

  • 2 formats: single-use and stainless steel
  • Leading perfusion device for mAb and rProtein production
  • Applications beyond perfusion - N-1 perfusion, High Productivity Harvest

How ATF works

award-winning ATF Technology


The XCell ATF® System is based on award-winning alternating tangential flow (ATF) technology, created by the action of a diaphragm moving upward and downward within a pump head, connected to a filter housing and attached to a bioreactor. Alternating tangential flow is attained by the action of a diaphragm pump.

XCell ATF® formats

XCell™ ATF Single-use System

XCell ATF® - Single-use 

100% cell retention, 80% faster implementation, no autoclave

The XCell ATF® Single-use System comes in multiple sizes from lab-scale to production-scale. Each system consists of a single-use device, a single-use hollow fiber filter cartridge, and a control system.

XCell™ ATF System - Stainless Steel

XCell ATF® - Stainless Steel

100% cell retention, high cell densities and product yields

The XCell ATF® System in stainless steel comes in multiple sizes from lab-scale to production-scale. Each system consists of a diaphragm pump in a stainless steel housing, a single-use hollow fiber filter cartridge, and a control system.


Enables continuous processing, provides clarified harvest ready for capture chromatography

High performance

100% cell retention, high cell concentration and viability; connects to any bioreactor and can be used in a variety of applications


Gentle action reduces cell stress, increases cell viability and productivity;  Self-cleaning, reduces biofilm build up on the hollow fiber filter

single-use format

Reduces implementation time by up to 80%, no autoclave


From process development-scale to production-scale


Field Applications and Service Specialists provide worldwide support

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system.  We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

about repligen manufacturing

perfusion and fed-batch applications

Long-term Perfusion

Perfusion, or upstream continuous processing, has been used since the 1980s. High cell density perfusion with XCell ATF® devices delivers higher cell concentration and cell viability.

N-1 Perfusion

N-1 perfusion refers to the intensification of cell growth in the N-1 bioreactor prior to the production N bioreactor, whh remains in fed-batch mode. This process enables high cell density seeding of the production bioreactor.

High Productivity Harvest

High Productivity Harvest (HPH) is a novel application developed by Repligen that enables you to harvest a fed-batch bioreactor in a fully sterile manner while eliminating both centrifugation and depth filtration.


Expand All

Frequently asked questions | perfusion