products

What is Slope Spectroscopy®?

A Unique and Patented Variable Pathlength Technology (VPT)

Providing a more efficient method for analyzing at-line and in-line processes, slope spectroscopy is a UV-based technology that serves as an alternative to the Beer-Lambert Law that focuses on pathlength as the variable that determines concentration.

Why Choose Slope Spectroscopy?

Decrease Errors

Eliminates reliance on a single fixed pathlength measurement.

Reduces the need for manual sample dilution or concentration adjustment.

Helps minimize operator-dependent variability and error.

Increase Accuracy

Captures absorbance data across multiple pathlengths.

Provides a more complete view of sample behavior and concentration.

Supports more reliable and consistent measurement results.

Save Time

Delivers concentration results in less than two minutes.

Automates analysis so users can focus on sample preparation and reporting.

Adjusts pathlength automatically based on absorbance changes.

How does Slope Spectroscopy work?

Called Slope Spectroscopy due to the slope this technique creates when there is a change in absorbance over a change in pathlength, this method leverages the power and flexibility of variable pathlength technology to create rapid and robust concentration measurements.

UV-based, this method analyzes samples at variable pathlengths using a precision linear stage to efficiently collect multiple absorbance data points to calculate a slope regression. Generating a slope value (absorbance over pathlength) allows you to quantify the concentration with higher accuracy, rather than relying on a single data point using absolute absorbance.

Unlike traditional spectroscopy — where the concentration is the variable — our method uses the pathlength as the variable, allowing the concentration to remain constant. When the concentration remains constant, you are able to eliminate dilution and decrease the chances of contamination and that creates a more reliable end result and product.

Traditional UV

Diagram illustrating traditional UV‑visible spectroscopy, showing light absorbance through a sample and corresponding absorbance spectrum.

Traditional spectroscopy uses concentration as the variable and a single data point using absolute absorbance

Slope Spectroscopy Method

Diagram illustrating slope‑based UV spectroscopy using a variable pathlength measurement, showing light source, sample vessel, detector, and absorbance versus pathlength relationship.

The Slope Spectroscopy Difference

A different approach to UV-Vis

Reduce Process Steps for Simplicity and Speed

Speak to a specialist today on how to start saving time and resources in your process using Slope Spectroscopy vs. traditional UV-Vis systems

Speak to an expert