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KrosFlo® KMPi TFF System

Designed for pilot-scale purification using tangential flow filtration (TFF), the KrosFlo® KMPi System delivers precise process controls and accurate data collection.
       •  100mL - 500L volume
       •  0.049 m2 - 5.1 m2 filtration area

TFF for Pilot scale purification


Designed for 100mL - 500L purification applications, the KrosFlo® KMPi TFF System from Repligen is designed for robust pilot-scale downstream ultrafiltration and microfiltration.

Fully integrated and ready to use out of the box, the KMPi provides consistent and accurate flow rates with a range of 0.01 to 13.0 LPM. 

How hollow fiber TFF works


The fully integrated KrosFlo® KMPi TFF System consists of a digital peristaltic pump, multi-language interface with a graphical LCD display digital pressure monitor for automated alarm and shut-off controls, KMPi Pump head, scale for automated alarms and shut-off controls, Automatic Backpressure Valve, module stand, and proprietary KF Comm Software. The KrosFlo® KMPi provides consistent and accurate flow rates with a range of 0.01 to 13.0 LPM. 

KrosFlo® KMPi System
with Hollow Fiber Filters

100mL - 500L volume
0.049 m2 - 5.1 m2 filtration area

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KrosFlo® KMPi System
with Flat Sheet Cassettes

500mL - 500L+ volume
0.5 m2 - 2.5 m2 filtration area

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Fully integrated

Includes filters, pumps, process monitors, software and graphic display


ProConnex® custom flow paths, 4 filter module configurations, 4 membrane chemistries, 2 effective filter lengths


Compatible with KrosFlo® TFF systems from process development to commercial manufacturing


Use with hollow fiber filters or flat sheet cassettes

KONDUiT walkaway automation

KONDUiT add-on module for automated process control of  conductivity, UV and temperature


Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

Automatic Back pressure control Valve

Unique to Repligen, the KrosFlo® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.

  • Automatically adjusts tubing pinch distance according to user-defined set-points for inlet, retentate, permeate or transmembrane pressures (TMP) when attached to the ProConnex® flow path
  • Maintains a uniform TMP throughout the process run, decreasing membrane fouling
  • Controls the permeate pressure and flow during microfiltration applications
  • Releases and tightens the pinch valve as solution viscosity increases or decreases during a process
  • Can be set to start pinching from the open, middle, or closed position
  • Compatible with tubing sizes 13, 14, 16, 25, HP15, 17, HP24, 18, HP35, 36, and HP36


Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration.  Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.

ProConnex® Flow Paths


KF Comm 2 Software, backward compatible with legacy KrosFlo® KR2i and KrosFlo® KMPi Systems, is conveniently installed on any computer using the Windows Operating System. The software is comptaible with Chrome, Mozilla, Firefox and Microsoft Explorer browsers and is always current with automatic software and firmware updates. 

  • Proprietary software optimized for KrosFlo® TFF Systems
  • Validated for GMP Processes with optional 21 CFR Part 11 option
  • Intuitive workflow with 13 modes of operation

KF Comm 2 Software

Compatible Filter Modules

Volume Inlet/ Outlet Side
Effective Length (cm) Total
Length (cm)

MiniKros Sampler

MiniKros-Sampler.png Batch TFF of small volumes 3 L -15 L 0.75" TC 0.75" TC 1 x FL
2 x FL
3 x FL
490 – 1000 cm2
1000 - 2200 cm2
1600 - 3400 cm2


MiniKros.png Pilot-scale TFF 5 L - 50 L 1.5" TC 0.75" TC 1 x FL
2 x FL
3 x FL
0.16 - 0.31 m2
0.32 - 0.65 m2
0.51 - 1.0 m2


KrosFlo.png Production-scale TFF 10 – 100L 3" TC 1.5" TC 1 x FL
2 x FL
3 x FL
0.78 - 1.6 m2
1.65 - 3.25 m2
2.55 - 5.10 m2

Compatible Flat Sheet Cassettes

TangenX™ SIUS™
Flat Sheet Cassettes

Membrane surface areas

0.5 meter2
1.5 meter2
2.5 meter2

KONDUiT add-on Monitor

Monitor and evaluate conductivity, UV and temperature for automated process controls. KONDUiT is an add-on module ready to use with the KrosFlo® KR2i and KMPi TFF Systems, with the ability to scale from 2mL to 500L process volumes.

KONDUiT adds:

  • End point conductivity control for UF /DF
  • End point UV control for diafiltering free drug from drug delivery particles
  • End point UV control for concentration of certain molecules
  • UV alarm for membrane breakthrough
  • A260 and A280 light sources available

*UV components sold separately



Conductivity Range 0.1 to 100 mS/cm
Temperature Range 0-70°
Pressure Range up to 75 psi (5 bar)
Dimensions (L x W x H): 7¾" x 4¾" x 4½" 
Weight 1.6 kg 

Scalable from benchtop to production

The KrosFlo® family of TFF Systems for ultrafiltration and diafiltration supports scale up from process development to commercial manufacturing.

systems and Components

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  • IP 66 and NEMA 4X Enclosure
  • 650 RPM pump drive with reversible motor
  • 90 to 260 Vrms @ 50/60 Hz
  • Standard 13 LPM Pump Head
  • High Performance 17 LPM pump head (optional)
  • ETL Listed and CE/UL/cUL marked
  • Dimensions: 17.5 in x 11in x 13in 
    (445 x 230 x 330 mm)
  • Shipping weight: 40 lbs (18kg)


  • Measures and displays 4 pressures: Inlet Pressure, Outlet Pressure, Permeate Pressure, Transmembrane Pressure (TMP)
  • User-defined pressure alarm and stop set-points
  • Available with many different connector types and materials of construction


Maintain user-defined feed weight set-points, and total permeate mass during TFF processes.


User-defined pressure alarms help ensure maximum sample recovery and  membrane integrity.

KrosFlo® KR1 Auxiliary Pump

Auxiliary pumps automatically increase or decrease buffer/product addition to feed reservoir.

TangenX™ SIUS™ Flat Sheet Cassette Clamps

Stainless steel plates designed to be used with TangenX™ SIUS™ Cassettes and filter plate inserts.


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