Designed for pilot-scale purification using tangential flow filtration (TFF), the KrosFlo® KMPi System delivers precise process controls and accurate data collection.
• 100mL - 500L volume
• 0.049 m2 - 5.1 m2 filtration area
TFF for Pilot scale purification
Designed for 100mL - 500L purification applications, the KrosFlo® KMPi TFF System from Repligen is designed for robust pilot-scale downstream ultrafiltration and microfiltration.
Fully integrated and ready to use out of the box, the KMPi provides consistent and accurate flow rates with a range of 0.01 to 13.0 LPM.
KrosFlo® KMPi TFF SYSTEM
The fully integrated KrosFlo® KMPi TFF System consists of a digital peristaltic pump, multi-language interface with a graphical LCD display digital pressure monitor for automated alarm and shut-off controls, KMPi Pump head, scale for automated alarms and shut-off controls, Automatic Backpressure Valve, module stand, and proprietary KF Comm Software. The KrosFlo® KMPi provides consistent and accurate flow rates with a range of 0.01 to 13.0 LPM.
Fully integrated
Includes filters, pumps, process monitors, software and graphic display
Configurable
Pro-Connex® custom flow paths, 4 filter module configurations, 4 membrane chemistries, 2 effective filter lengths
Scalable
Compatible with KrosFlo® TFF systems from process development to commercial manufacturing
Flexible
Use with hollow fiber filters or flat sheet cassettes
KONDUiT walkaway automation
KONDUiT add-on module for automated process control of conductivity, UV and temperature
Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.
Automatic Back pressure control Valve
Unique to Repligen, the KrosFlo® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.
- Automatically adjusts tubing pinch distance according to user-defined set-points for inlet, retentate, permeate or transmembrane pressures (TMP) when attached to the Pro-Connex® flow path
- Maintains a uniform TMP throughout the process run, decreasing membrane fouling
- Controls the permeate pressure and flow during microfiltration applications
- Releases and tightens the pinch valve as solution viscosity increases or decreases during a process
- Can be set to start pinching from the open, middle, or closed position
- Compatible with tubing sizes 13, 14, 16, 25, HP15, 17, HP24, 18, HP35, 36, and HP36
Configurable Pro-Connex® Flow Path
Enhanced through an engineering design process, Pro-Connex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration. Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.
Proprietary KF Comm Software
KF Comm Software
- Automatically collects process data
- Provides real-time overviews of the filtration process
Microsoft Surface Pro 4 with Excel
- Automatically graphs data in Excel-based software
- 21CFR Part 11 enabled when used with optional KF Comm Compliant Workbook and interface software package from OFNI Systems (ExcelSafe™)
Real-time operating parameters
- Time
- Feed Pressure
- Retentate Pressure
- Permeate Pressure
- Feed Flow Rate
- Diafiltration Flow Rate
- Permeate Flow Rate
- Feed Scale Weight
- Permeate Scale Weight
- Pump RPM
- Notes (Alarms, Process status, Functions)
- Pump Mode
Processing parameters
- Transmembrane Pressure (TMP)
- Pressure Drop (ΔP)
- Retentate Flow Rate
- Permeate Flux
- Volumetric Throughput (VT)
- Concentration Factor
- Shear
- Diafiltration Volumes
- Process Time
- UV, Conductivity, and Temperature (with KONDUiT)
Compatible Filter Modules
| Primary Application |
Volume | Inlet/ Outlet | Side Port |
Scale Length |
Effective Length (cm) | Total Length (cm) |
Surface area |
|
|---|---|---|---|---|---|---|---|---|
MiniKros Sampler |
||||||||
 |
Batch TFF of small volumes | 3 L -15 L | 0.75" TC | 0.75" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 47 70 |
490 – 1000 cm2 1000 - 2200 cm2 1600 - 3400 cm2 |
MiniKros |
||||||||
 |
Pilot-scale TFF | 5 L - 50 L | 1.5" TC | 0.75" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 46 70 |
0.16 - 0.31 m2 0.32 - 0.65 m2 0.51 - 1.0 m2 |
KrosFlo® |
||||||||
 |
Production-scale TFF | 10 – 100L | 3" TC | 1.5" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
23 46 69 |
0.78 - 1.6 m2 1.65 - 3.25 m2 2.55 - 5.10 m2 |
Compatible Flat Sheet Cassettes
KONDUiT add-on Monitor
Monitor and evaluate conductivity, UV and temperature for automated process controls. KONDUiT is an add-on module ready to use with the KrosFlo® KR2i and KMPi TFF Systems, with the ability to scale from 2mL to 500L process volumes.
KONDUiT adds:
- End point conductivity control for UF /DF
- End point UV control for diafiltering free drug from drug delivery particles
- End point UV control for concentration of certain molecules
- UV alarm for membrane breakthrough
- A260 and A280 light sources available
*UV components sold separately
| Conductivity Range | 0.1 to 100 mS/cm |
| Temperature Range | 0-70° |
| Pressure Range | up to 75 psi (5 bar) |
| Dimensions | (L x W x H): 7¾" x 4¾" x 4½" |
| Weight | 1.6 kg |
Scalable from benchtop to production
The KrosFlo® family of TFF Systems for ultrafiltration and diafiltration supports scale up from process development to commercial manufacturing.
KrosFlo® KR2i TFF System
2ml - 10L
KrosFlo® KMPi TFF System
1L - 500L
KrosFlo® KTF TFF System
500L - 5000L
systems and Components
- IP 66 and NEMA 4X Enclosure
- 650 RPM pump drive with reversible motor
- 90 to 260 Vrms @ 50/60 Hz
- Standard 13 LPM Pump Head
- High Performance 17 LPM pump head (optional)
- ETL Listed and CE/UL/cUL marked
- Dimensions: 17.5 in x 11in x 13in
(445 x 230 x 330 mm) - Shipping weight: 40 lbs (18kg)
Transducers
- Measures and displays 4 pressures: Inlet Pressure, Outlet Pressure, Permeate Pressure, Transmembrane Pressure (TMP)
- User-defined pressure alarm and stop set-points
- Available with many different connector types and materials of construction
Scales
Maintain user-defined feed weight set-points, and total permeate mass during TFF processes.
Monitor
User-defined pressure alarms help ensure maximum sample recovery and membrane integrity.
KrosFlo® KR1 Auxiliary Pump
Auxiliary pumps automatically increase or decrease buffer/product addition to feed reservoir.
TangenX™ SIUS™ Flat Sheet Cassette Clamps
Stainless steel plates designed to be used with TangenX™ SIUS™ Cassettes and filter plate inserts.
