REBEL XT At-line Media Analyzer
At-line Analysis for Cell Culture Media
Built on the proven foundation of the award winning REBEL analyzer, the REBEL XT System continues to challenge the limits of traditional lab testing—delivering the next level of at-line cell culture media analysis with greater reliability, streamlined usability, and tighter analytical performance. Designed for real-world cell culture media process development, it delivers faster, real-time insights into media composition—giving you the data you need, exactly where and when you need it.
Why wait for the lab? Break free from traditional lab delays. The REBEL XT at-line analyzer puts real-time media composition data at your fingertips—delivering results for over 20 key components in approximately 10 minutes, right where and when you need them.
Cell Culture Media Matters. Here’s Why.
Optimizing cell culture media composition is essential to advance process development and accelerate scale-up. The REBEL XT at-line analyzer sets a new standard for media analysis—delivering rapid, hassle-free insights that lead to deeper process understanding, faster decisions, and a quicker path to market.
Fresh Media Fingerprinting
Save money by avoiding risk of batch failure with fresh media formulation analysis.
Media Development
Know your cell nutrient requirements and accelerate media development timelines.
Process Development
Reduce process development timelines and improve productivity.
Process Monitoring
Get to the root cause of manufacturing process variations and decrease risk of wasted batches.
Process Modeling
Add the missing pieces to your predictive model for smarter DOE resulting in faster process development.
Reduce Downtime Between Experiments & Save Money
Fewer Bioreactor Runs, Less FTE Time, Shorter Development Time
The REBEL XT analyzer puts critical media composition data in your hands during the bioreactor run—not days later. By tracking nutrient changes in real time, you can optimize feeding strategies on the spot, boost cell growth, and improve product yield. The REBEL XT analyzer empowers you to make informed decisions that can significantly impact your biotherapeutic development, helping to save FTE hours, shorten development timelines, and cut costly rework.
Traditional Workflow With Core or External Lab Analytics:
REBEL XT Assisted Workflow:
*REBEL XT used in combination with modeling can lead to fewer bioreactor runs.
What If You Could Reduce Analytics from
3-6 Weeks to 10 Minutes?
Traditional Analytics: Can Take 3 to 6 Weeks
The traditional analytics process can take 3–6 weeks, which can lead to prolonged decision-making.
STEP ONE
Pull sample
STEP TWO
Filter cells
STEP THREE
Label, secure, collect, freeze samples (14 days), and fill out paperwork
STEP FOUR
Send frozen batch to core lab
STEP FIVE
Core lab defrosts, dilutes, preps derivatization reagents
STEP SIX
Core lab mixes samples with chemicals
STEP SEVEN
Reacted samples put into HPLC
STEP EIGHT
Run HPLC, acquire data
STEP NINE
Analyze data, summarize results
Report Generated
REBEL XT Analytics: Only Takes Approximately 10 Minutes
The REBEL XT provides rapid analytics, enabling quick decision-making.
STEP ONE
Pull sample
STEP TWO
Spin out cells and dilute
STEP THREE
Insert sample into REBEL XT
STEP FOUR
Start REBEL XT
Report Generated!
Remove Data Analysis Bottlenecks
REBEL and REBEL XT users with access to the JMP statistical software package are able to expedite high-throughput data analysis by data visualization.
REBEL XT Data Processing
Quantitative numbers for each analyte, from each sample directly from the REBEL XT.
JMP Statistical Discovery
Expedite high-throughput data analysis from REBEL XT with JMP statistical discovery software.
Consumables That Don’t Consume Your Time
Easy Sample Preparation
Just filter, spin, and dilute your samples. There’s no chemical reaction, and it works for both low and high sample throughput needs.
Low-Effort Method Development
Use the touch screen to get the sample running. BGE carries the samples through the microchip for ESI-MS detection.
Simple System Check
The REBEL XT makes it easy with a built-in quantification calibration and system performance check with REBEL XT SMArt standard.
Comprehensive Analysis
20+ analytes including amino acids, dipeptides, and choline are quantified in as little as 10 minutes per sample.
How Will You Use the REBEL XT Analyzer?
Monitor amino acids and media composition over the course of the cell culture process. Identify depleted nutrients and refine supplementation strategies on the fly. Correlate media composition to product yield and quality.
Biologics
Rapid spent media analysis with actionable intelligence for enhancing cell productivity and desired CQAs.
Cell Therapy
One of the keys to cell therapy process development lies in the cell culture media used to culture the cells after isolation.
Gene Therapy
Cut development times to screen and optimize media to boost viral vector production and improve transduction.
Fermentation
At-line bacterial media analysis that handles supplements with ease.
Synthetic Biology
Understand growth media components at any scale, from cellular agriculture to chemicals and materials.
Decoding Cell Culture Media Composition
REBEL XT analyzer offers a groundbreaking approach to the once insurmountable challenge of analyzing the intricacies of cell culture environments. Gain an unprecedented view into a wide spectrum of critical components in cell culture media. Each of these play a unique role in cellular function and metabolism, and REBEL XT is your key to streamlining your research workflows.
REBEL XT Analyte Panel
- Alanine
- Alanyl-Glutamine
- Arginine
- Asparagine
- Aspartic acid
- Choline
- Cystine
- Glutamic acid
- Glutamine
- Glycine
- Histidine
- Isoleucine
- Leucine
- Lysine
- Methionine
- Phenylalanine
- Proline
- Serine
- Threonine
- Tryptophan
- Tyrosine
- Valine
REBEL XT analyzer is used for rapid at-line analysis of cell culture media components including 19 amino acids, 2 dipeptides, and choline. Amino acids are critical for cellular metabolism and are often present in media used for expansion of mammalian, microbial, and insect cell lines such as CHO, HEK 293, E. coli, Sf9, Sf21, T cells, and stem cells.
With REBEL XT analyzer, you can quickly and easily quantify concentrations of over 20 media components in fresh and spent cell culture media. Its small footprint and award-winning design lets you conveniently analyze media samples on the bench, next to the bioreactors, without leaving your lab. This pioneering tool is powered by the advanced CE-MS (Capillary Electrophoresis – Mass Spectrometry) technology and produces easy-to-understand reports with concentrations of over 20 essential media components in 10 minutes. There is minimal sample preparation, and no prior expertise is required to operate the device.
The REBEL XT cell culture media analyzer is pivotal in accelerating process development, and supports Continuous Process Verification (CPV). Whether your work involves development or manufacturing of therapeutic recombinant proteins (e.g. monoclonal antibodies, bi-specific antibodies, antibody drug conjugates, biosimilars), cell and gene therapies, or cultivated meats, the REBEL XT can help streamline your cell culture media analysis workflows. By implementing REBEL XT at your lab, you will eliminate the need for sending out media samples to an external lab and having to wait weeks for results. Instead, you will be able to perform cell culture media analysis, in minutes, as often as you’d like, cost-effectively, with minimal training and no method development.
Having media composition data at your fingertips, helps you shave weeks off the process development time, better optimize your cell culture or fermentation processes, and monitor production runs.
REBEL XT helps advance cell therapy process development with on-demand analysis of cell culture media, right at the bench. REBEL XT allows you unprecedented insight into variations in amino acid and nutrient concentrations as your cells grow.
REBEL XT accepts both septa-capped vials and 96-well plates (two 96-well plates are provided with each REBEL XT kit). The samples and standards are kept at 5 °C inside the REBEL XT’s autosampler and there are plate covers included in the kits to prevent evaporation. Once your samples are loaded, you can either manually enter your sample information using the REBEL XT’s touchscreen or import the information using the pre-filled Batch Runsheet Template file.
Analysis reports are saved in either CSV or PDF file formats to REBEL XT’s internal memory or user-specified location on the network. We highly recommend exporting the analysis results/reports to a network location to ensure data integrity and prevent loss of data from overwriting when internal device memory gets full.
Each REBEL SMArt Kit can be used to analyze up to 200 samples.
The media sample volume is typically between 10-100 uL depending on the sample container (2ml autosampler vials or 96 well plates). Please note that a minimum 10x sample dilution is required.
The dynamic range for most analytes is 5-100 uM.
If a PQ has already been run, it would take 10 minutes for a single sample run and 4 minutes for subsequent replicates.
Yes, REBEL XT is 21 CFR part 11 complaint. REBEL XT records and timestamps all the critical system events (i.e., user logins/logouts, password changes, system modifications, completion of assays, maintenance, and performance qualifications). The audit log files are automatically exported to the system’s configured network location. Secondary policies/procedures should be implemented to ensure the integrity of the audit log files once they have been exported to a location on your network.
You can place and run the REBEL XT on a sturdy cart but please make sure that the cart can support REBEL XT’s weight and footprint. Ideally there should be a power outlet and a network port nearby to avoid any trip hazards. If you would like to make your REBEL XT mobile, reach out to us and we will point you in the right direction.