MAVERICK In-line Critical Process Parameter Monitoring
Process Analytical Technology for In-line Bioprocess Analysis
This easy-to-use process analytical technology device offers in-line bioprocess analysis and control with no complex modeling. Measure multiple critical parameters across media, across processes, across mammalian cell lines, across scales.
All In-line Gain, No Modeling Pain
MAVERICK is powered by Raman spectroscopy and a purpose-built de novo model. The de novo model is explicitly built on media chemistries and device physics, not on empirical data. It automatically interprets the spectra and quantifies process parameters enabling simultaneous measurements of glucose, lactate, and total biomass. This is achieved without the costs, complexities, and risks associated with conventional Process Analytical Technology (PAT) methods. There is no need to grapple with complex empirical models, reagents, or unreliable auto-sampling systems to implement in-line bioprocess analysis.
How the De Novo Model Works
The MAVERICK measurements are based on a de novo model. This model was developed from first principles without empirical observation/calibration in bioprocesses with any particular media. The de novo model considers both static and dynamic parameters measured during the actual bioprocess runs. One of the static parameters is a formulary which includes the spectral characteristics of many chemical components in media. These include glucose, lactate, and other cell culture media components detectable by Raman. The dynamic parameters include adapting to changes in environmental factors such as ambient light, temperature, and turbidity, including cell based fluorescence. It also accounts for the characteristics of each unique device from an optical, electronic, and measurement error model perspective.
Real-time Analysis in Minutes
Measure critical bioprocess parameters now, not in weeks or months. No bioprocess runs to collect Raman spectra. No modeling required. There’s an easier way–with Maverick's purpose-built de novo model. Get actionable process parameters in three easy steps: calibrate, autoclave, and start your bioprocess run. These videos show you how.
Calibration
Utilize two standards to calibrate and qualify MAVERICK.
Autoclave
Prepare your bioreactor and MAVERICK probe.
Start Bioprocess Run
Connect the MAVERICK probe to your bioreactor and begin monitoring process parameters. Measurements will be displayed in minutes.
Process Analytical Technology (PAT) Made Easy
While in-line process analytical technology (PAT) offers many benefits, adoption has been hindered by technical challenges, integration issues, and significant implementation costs.
MAVERICK addresses all these issues, ushering in an era of accessible, continuous monitoring of multiple parameters. With Maverick, your team can stay at the forefront of Bioprocessing 4.0 and fully harness the advantages of real-time in-line bioprocess analysis (bioreactor monitoring) and control. Intuitive setup and user-friendly interface means there is no training barrier to overcome.
MAVERICK– Easy to Learn, Easy to Use
MAVERICK is intuitively designed for ease of use, requiring no prior experience with Raman spectroscopy or chemometric modeling. It features a user-friendly hub with a high-resolution color touchscreen, CFR 21 Part 11 compliant software, and an intuitive interface that simplifies in-line bioprocess analysis. With MAVERICK, mastering bioprocess monitoring is straightforward and efficient.
Instant Raman,
Ready in Minutes
MAVERICK offers all the advantages of Raman-based process analytical technology (PAT) without the cost and complexities associated with developing “black box” empirical calibration models, required by conventional process spectroscopy-based methods. While it can take months and a dedicated team of experts to build and validate a robust empirical calibration model—MAVERICK only takes a simple two-point probe calibration to begin monitoring multiple key process parameters in most types of bioreactors. Just take it out of the box, and you’re already halfway through the setup process.
MAVERICK Setup: Complete in Less Than 1 Day
Start monitoring multiple process parameters in just four simple steps
STEP ONE
Take MAVERICK out of the box and connect components
STEP TWO
Perform two-point calibration with the standard
STEP THREE
Autoclave the probe body with the bioreactor
STEP FOUR
Reconnect the probe
You Are Ready to Monitor Multiple Process Parameters
Traditional Raman Setup: Takes 18 to 60 Months
The calibration model requires revalidation for any significant change in process conditions
STEP ONE
Unbox the instrument and connect the components
STEP TWO
Calibrate and standardize probe optics
STEP THREE
Autoclave the probe with bioreactor
STEP FOUR
Reconnect the probe and prepare the bioreactor runs
STEP FIVE
Collect Raman spectra from multiple bioreactor runs
STEP SIX
Extract samples and collect reference measurements
STEP SEVEN
Build multivariate empirical calibration model
STEP EIGHT
Validate model performance
You Are Ready to Monitor Process Parameters
Already Have Raman Implemented & Having Trouble?
MAVERICK is an easily deployable and cost-effective Raman solution, compatible with both empirical calibration and out-of-the-box measurements utilizing the de novo model. This enables easy implementation across multiple processes, sites, scales and applications. Scaling out real-time, in-line monitoring capabilities provides additional spectral data, decreased time to robust models, and enables control of CPPs.
MAVERICK's de novo model allows for easy measurement method validation because it is a commercial off-the-shelf system (COTS), unlike empirical models. This delivers a robust and scalable Process Analytical Technology (PAT) solution that helps advance your bioprocess through the pipeline to commercialization.
Multiplex Flex
Connect up to six measurement modules to a single MAVERICK hub to monitor process conditions and control feeds across multiple bioreactors independently and simultaneously in real-time. This setup saves valuable bench space and boosts efficiency.
Walk-Away Automation with MAVERICK
Control feeding pumps directly or relay the measurements to an existing automation or data acquisition system. Integration is simplified with analog and digital connectivity via Ethernet or OPC UA. Optional remote monitoring allows you to securely check on your bioreactors from anywhere, anytime, 24/7.
Data Visualization with MAVERICK and JMP Software
MAVERICK users can quickly visualize time-course data and compare different runs using the JMP statistical software package.
Maverick Data Processing
MAVERICK data files are rich in data from in-line measurements of glucose, lactate and biomass. The values in g/L, biomass units are provided in 1-minute intervals.
908 Devices Add-in,
with JMP Statistical Discovery
Visualize time-course data for glucose, lactate, and biomass and compare runs.
MAVERICK Consumables & Services
Ensure your MAVERICK operates at peak efficiency with our range of consumables and services. We offer replacement immersion probes, calibration standards, Installation Qualification/Operational Qualification (IQ/OQ) support, and comprehensive service plans designed to maintain your favorite process analytical technology tool in optimal condition.
How Will You Use MAVERICK?
With MAVERICK you get in-line, real-time, and robust bioreactor monitoring of multiple key process parameters in biopharmaceutical manufacturing of monoclonal antibodies, recombinant proteins, and viral vectors utilizing CHO and HEK293 cell lines in a variety of media types.
Biologic Production
Enhance cell culture process development and manufacturing through in-line monitoring and control.
Gene Therapy
Optimize viral vector production with real-time monitoring of critical process parameters.
MAVERICK is intended for bioprocessing applications in process development and biopharmaceutical manufacturing, enabling cell culture automation in biologic production for CHO or HEK293 mammalian cell line and media. For example, MAVERICK can help optimize viral vector titer in lentiviral vector production, AAV production, and other viral vector manufacturing applications.
MAVERICK works with CHO and HEK293 cell lines and media.
MAVERICK autoclavable immersion probe is 220 mm long with 12 mm outside diameter, making it compatible with most 250 mL to 10L bioreactors. The PG 13.5 compression fitting interfaces with the standard bioreactor sensor ports and enables adjustment of the immersion depth (minimum 40 mm immersion depth is required).
Yes. Each MAVERICK Hub connects and controls up to six Measurement Modules, supporting up to six individually or jointly operated bioreactors. Unlike other process Raman spectroscopy analyzers that employ optical switches or multi-channel design, MAVERICK preserves operational redundancy in multi-bioreactor applications.
The probe is calibrated using the two standards in the Calibration Kit. Calibration is performed prior to autoclaving the probe, before each bioreactor run. Each probe has a built-in memory chip that stores its calibration coefficients and verifies that the correct probe was re-attached post-sterilization.
On-board analog/digital controller offers optional direct control of feed pumps. Alternatively, the measured values can be sent to an existing bioreactor controller, SCADA, or data acquisition system via Analog outputs or OPC UA protocol.
Probe barrels are autoclavable or steam sterilizable and should be replaced after 10 autoclave cycles to ensure the probe maintains its integrity.
Normally, interpreting the spectrum from Raman spectroscopy requires the development of empirical multivariate calibration models (chemometric modeling) specific to analytes of interest. This requires multiple bioreactor runs to acquire real-time spectral data, manually collect numerous samples from the bioreactors for at-line/off-line analysis, and then utilize statistical analysis to correlate the Raman spectra to the at-line/off-line measurements. Naturally, this process is very time consuming, expensive, and complicated, making Raman-based bioprocess monitoring inaccessible to most biopharma labs.
MAVERICK purpose-built De Novo Model does not need to be trained on empirical process data. The model quantifies analyte concentration by accurately inferring peaks, associated with the analytes of interest based on a priori knowledge and the baseline from water, other mixture components, and instrumentation in the spectra.
MAVERICK devices and support are available for purchase directly from 908 Devices in North America and Europe, and through a network of distributors globally.