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Solutions for Adeno-Associated Virus (AAV) Manufacturing

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Adeno-associated virus (AAV) is the most common viral vector used in gene therapy products due to demonstrated safety, low immunogenicity and long-term transgene expression. Typical cells used are HEK-293, HeLa, Vero, and SF9.

Repligen solutions for AAV Manufacturing

Needs and challenges:

  • Downstream operations are more challenging due to the cell lysis step.
  • Downstream processes can include sterile filtration although closed, aseptic processing is preferred.
  • Different serotypes can bring unique process challenges.

Repligen solutions can help overcome obstacles in AAV bioprocessing.

Click on the unit operation below to find productivity and throughput solutions you can implement today. 

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XCell ATF®
Systems

Scalable, single-use cell retention device for cell culture process intensification and media exchange

Higher pre-transfection viable cell density and total capsid titer

 

XCell_SU_2-6-10.jpg KrosFlo_Pro-Connex.jpg hf_pardot.jpg KrosFlo-tff-systems.jpg

XCell ATF®
Systems

ProConnex®
Flow Paths

Spectrum® Hollow
Fiber Filters

KrosFlo® TFF
Systems

Scalable, single-use cell retention device for cell culture process intensification and media exchange

Higher pre-transfection viable cell density and total capsid titer

Sterile, disposable, sensor-integrated and ready-to-operate

Compatible with hollow fiber and flat sheet filters

Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk

Wide range of formats, chemistries and pore sizes

Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation

Fully automated, easy to operate

Scalable from bench to production

Compatible with hollow fiber and flat sheet TFF

Low hold-up volumes

 

XCell_SU_2-6-10.jpg KrosFlo_Pro-Connex.jpg hf_pardot.jpg KrosFlo-tff-systems.jpg SOLO2_1.jpg

XCell ATF®
Systems

ProConnex®
Flow Paths

Spectrum® Hollow
Fiber Filters

KrosFlo® TFF
Systems

SoloVPE™
Device

Scalable, single-use cell retention device for cell culture process intensification and media exchange

Higher pre-transfection viable cell density and total capsid titer

Sterile, disposable, sensor-integrated and ready-to-operate

Compatible with hollow fiber and flat sheet filters

Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk

Wide range of formats, chemistries and pore sizes

Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation

Fully automated, easy to operate

Scalable from bench to production

Compatible with hollow fiber and flat sheet TFF

Low hold-up volumes

Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution

Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability

KrosFlo_Pro-Connex.jpg hf_pardot.jpg KrosFlo-tff-systems.jpg SIUSgamma_Front.jpg SOLO2_1.jpg

ProConnex®
Flow Paths

Spectrum® Hollow
Fiber Filters

KrosFlo®
TFF Systems

TangenX™ SIUS™ Gamma Flat Sheet Cassettes

SoloVPE™
Device

Sterile, disposable, sensor-integrated and ready-to-operate

Compatible with hollow fiber and flat sheet filters

Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk

Wide range of formats, chemistries and pore sizes

Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation

Fully automated, easy to operate

Scalable from bench to production

Compatible with hollow fiber and flat sheet TFF

Low hold-up volumes

Closed, ready-to-use, single-use cassettes

High flux membranes increase yield, reduce process times, promote consistency, minimize costs

Ideal for AAV processes where large volumes need to be concentrated 20-60x prior to chromatography

Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution

Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability

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OPUS® 
Columns

SoloVPE™
Device

Ready-to-use, no packing expertise needed

Pack any resin for AAV, Lentivirus, or plasmid purification (including custom resins)

0.5 - 30 cm IDs | 5 - 30 cm bed heights

Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution

Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability

KrosFlo_Pro-Connex.jpg hf_pardot.jpg KrosFlo-tff-systems.jpg SIUSgamma_Front.jpg SOLO2_1.jpg

ProConnex®
Flow Paths

Spectrum® Hollow
Fiber Filters

KrosFlo® TFF
Systems

TangenX™ SIUS™ Gamma Flat Sheet Cassettes

SoloVPE™
Device

Sterile, disposable, sensor-integrated and ready-to-operate

Compatible with hollow fiber and flat sheet filters

Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk

Wide range of formats, chemistries and pore sizes

Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation

Fully automated, easy to operate

Scalable from bench to production

Compatible with hollow fiber and flat sheet TFF

Low hold-up volumes

Closed, ready-to-use, single-use cassettes

High flux membranes increase yield, reduce process times, promote consistency, minimize costs

Ideal for AAV processes where large volumes need to be concentrated 20-60x prior to chromatography

Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution

Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability

SOLO2_1.jpg
SoloVPE™
Device

Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution

Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability

Resources

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