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Solutions for Lentivirus Manufacturing

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Lentivirus is an efficient gene delivery vector because of its high-efficiency infection of dividing and non-dividing cells, low immunogenicity and long-term stable expression of a transgene. Examples of lentiviruses are HIV, SIV, and FIV.

Repligen solutions for Lentivirus manufacturing

Needs and challenges:

  • Tools and processes are needed that take into consideration the shear/pH/temperature sensitivity of Lentivirus.
  • Closed and aseptic processing is ideal; a sterile flow path is key to application.
  • Full or partial media exchange may be needed for optimized viral genome titer.

Repligen solutions can help overcome obstacles in Lentivirus bioprocessing.

Click on the unit operation below to find productivity and throughput solutions you can implement today. 

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XCell ATF® 
Systems

Scalable, single-use cell retention device for cell culture process intensification and media exchange

Higher pre-transfection viable cell density and total capsid titer

 

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XCell ATF® 
Systems

ProConnex® 
Flow Paths

Spectrum® Hollow
Fiber Filters

KrosFlo® TFF
Systems

Scalable, single-use cell retention device for cell culture process intensification and media exchange

Higher pre-transfection viable cell density and total capsid titer

Sterile, disposable, sensor-integrated and ready-to-operate

Compatible with hollow fiber and flat sheet filters

Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk

Wide range of formats, chemistries and pore sizes

Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation

Fully automated, easy to operate

Scalable from bench to production

Compatible with hollow fiber and flat sheet TFF

Low hold-up volumes

 

XCell_SU_2-6-10.jpg KrosFlo_Pro-Connex.jpg hf_pardot.jpg KrosFlo-tff-systems.jpg SOLO2_1.jpg OPUS_Family_Large-scale.jpg

XCell ATF®
 Systems

ProConnex® 
Flow Paths

Spectrum® Hollow
Fiber Filters

KrosFlo® TFF
Systems

SoloVPE™ 
Device

OPUS® 
Columns

Scalable, single-use cell retention device for cell culture process intensification and media exchange

Higher pre-transfection viable cell density and total capsid titer

Sterile, disposable, sensor-integrated and ready-to-operate

Compatible with hollow fiber and flat sheet filters

Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk

Wide range of formats, chemistries and pore sizes

Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation

Fully automated, easy to operate

Scalable from bench to production

Compatible with hollow fiber and flat sheet TFF

Low hold-up volumes

Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution

Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability

Ready-to-use, no packing expertise needed

Pack any resin for AAV, Lentivirus, or plasmid purification (including custom resins)

0.5 - 30 cm IDs | 5 - 30 cm bed heights

KrosFlo-TFDF-Lab-System
KrosFlo® TFDF™ 
Systems

Closed, single-use, automated, alternative to centrifugation and primary depth filtration

Ideal for Lentivirus processes: Improved yields low shear, low hold-up volumes

Scalable from 1 to 2000L

KrosFlo_Pro-Connex.jpg hf_pardot.jpg KrosFlo-tff-systems.jpg SOLO2_1.jpg

ProConnex® 
Flow Paths

Spectrum® Hollow
Fiber Filters

KrosFlo® TFF
Systems

SoloVPE™ 
Device

Sterile, disposable, sensor-integrated and ready-to-operate

Compatible with hollow fiber and flat sheet filters

Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk

Wide range of formats, chemistries and pore sizes

Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation

Fully automated, easy to operate

Scalable from bench to production

Compatible with hollow fiber and flat sheet TFF

Low hold-up volumes

Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution

Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability

opus_GT.jpg SOLO2_1.jpg

OPUS® 
Columns

SoloVPE™
Device

Ready-to-use, no packing expertise needed

Pack any resin for AAV, Lentivirus, or plasmid purification (including custom resins)

0.5 - 30 cm IDs | 5 - 30 cm bed heights

Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution

Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability

KrosFlo_Pro-Connex.jpg hf_pardot.jpg KrosFlo-tff-systems.jpg SOLO2_1.jpg

ProConnex® 
Flow Paths

Spectrum® Hollow
Fiber Filters

KrosFlo® TFF
Systems

SoloVPE™
Device

Sterile, disposable, sensor-integrated and ready-to-operate

Compatible with hollow fiber and flat sheet filters

Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk

Wide range of formats, chemistries and pore sizes

Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation

Fully automated, easy to operate

Scalable from bench to production

Compatible with hollow fiber and flat sheet TFF

Low hold-up volumes

Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution

Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability

SOLO2_1.jpg
SoloVPE™
Device

Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution

Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability

Resources

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Lentiviral vector manufacturing process enchancement utilizing TFDF™ technology
by Thomas Williams, Oliver Goodyear, Lee Davies, Carol Knevelman, Michael Bransby, Kyriacos Mitrophanous and James Miskin
Cell and Gene Therapy Insights, April 2020