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KrosFlo® TFDF® Systems |
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Closed, single-use, automated, alternative to centrifugation and primary depth filtration Ideal for Lentivirus processes: Improved yields low shear, low hold-up volumes Scalable from 1 to 2000L |
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SoloVPE® Device |
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Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution Automated plasmid/viral vector concentration measurement with increased accuracy and repeatability |
Lentiviral process intensification achieves a 250% overall lentiviral harvest yield increase over depth filtration. After first harvest, the process generates >90% lentiviral yield compared to 70% using depth filtration. Low shear TFDF® technology preserves HEK293 cell growth and enables a second harvest that results in 80% more yield. Process flux maintained at 750 LMH throughout without fouling. Transmembrane pressure maintained less than 3 psi across both harvests with one filter.
Higher product yield and transmission with HPH
Source: Cell & Gene Therapy Insights, 2000, 6, 455-467
Multiple harvests with the same filter while maintaining low TMP
Repligen Field Application Specialists (FAS) with expertise in Gene Therapy provide support from consultation to implementation globally.
Lentiviral vector manufacturing process enchancement utilizing TFDF™ technology
by Thomas Williams, Oliver Goodyear, Lee Davies, Carol Knevelman, Michael Bransby, Kyriacos Mitrophanous and James Miskin
Cell and Gene Therapy Insights, April 2020
Gene Therapy Manufacturing 2.0
by René Gantier, PhD, Director of Gene Therapy Technologies, Repligen Corporation
Repligen Corporation
April 2021
AAV Studies Deploying a Closed, Irradiated, Single-use Flat Sheet TFF Device
by David Bianchi, Carl Breuning, Michael LaBreck, Shelly Parra, Mary Jo Wojtusik, Repligen Corporation
Alex Meola, Michael Mercaldi, Thomas Thiers, Homology, Medicines Inc.
Repligen Corporation
Homology, Medicines Inc.
July 2020