Lentivirus is an efficient gene delivery vector because of its high-efficiency infection of dividing and non-dividing cells, low immunogenicity and long-term stable expression of a transgene. Examples of lentiviruses are HIV, SIV, and FIV.
Repligen solutions for Lentivirus manufacturing
Needs and challenges:
- Tools and processes are needed that take into consideration the shear/pH/temperature sensitivity of Lentivirus.
- Closed and aseptic processing is ideal; a sterile flow path is key to application.
- Full or partial media exchange may be needed for optimized viral genome titer.
Repligen solutions can help overcome obstacles in Lentivirus bioprocessing.
Click on the unit operation below to find productivity and throughput solutions you can implement today.
Expand All
Expand All
 |
|---|
|
Scalable, single-use cell retention device for cell culture process intensification and media exchange Higher pre-transfection viable cell density and total capsid titer |
|
 |
 |
 |
|---|---|---|---|
|
Scalable, single-use cell retention device for cell culture process intensification and media exchange Higher pre-transfection viable cell density and total capsid titer |
Sterile, disposable, sensor-integrated and ready-to-operate Compatible with hollow fiber and flat sheet filters Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk |
Wide range of formats, chemistries and pore sizes Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation |
Fully automated, easy to operate Scalable from bench to production Compatible with hollow fiber and flat sheet TFF Low hold-up volumes |
|
|
|
|
 |
 |
|---|---|---|---|---|---|
|
Scalable, single-use cell retention device for cell culture process intensification and media exchange Higher pre-transfection viable cell density and total capsid titer |
Sterile, disposable, sensor-integrated and ready-to-operate Compatible with hollow fiber and flat sheet filters Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk |
Wide range of formats, chemistries and pore sizes Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation |
Fully automated, easy to operate Scalable from bench to production Compatible with hollow fiber and flat sheet TFF Low hold-up volumes |
Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability |
Ready-to-use, no packing expertise needed Pack any resin for AAV, Lentivirus, or plasmid purification (including custom resins) 0.5 - 30 cm IDs | 5 - 30 cm bed heights |
 |
| KrosFlo® TFDF™ Systems |
|---|
|
Closed, single-use, automated, alternative to centrifugation and primary depth filtration Ideal for Lentivirus processes: Improved yields low shear, low hold-up volumes Scalable from 1 to 2000L |
|
|
|
|
|---|---|---|---|
|
Sterile, disposable, sensor-integrated and ready-to-operate Compatible with hollow fiber and flat sheet filters Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk |
Wide range of formats, chemistries and pore sizes Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation |
Fully automated, easy to operate Scalable from bench to production Compatible with hollow fiber and flat sheet TFF Low hold-up volumes |
Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability |
 |
|
|---|---|
|
Ready-to-use, no packing expertise needed Pack any resin for AAV, Lentivirus, or plasmid purification (including custom resins) 0.5 - 30 cm IDs | 5 - 30 cm bed heights |
Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability |
|
|
|
|
|---|---|---|---|
|
Sterile, disposable, sensor-integrated and ready-to-operate Compatible with hollow fiber and flat sheet filters Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk |
Wide range of formats, chemistries and pore sizes Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation |
Fully automated, easy to operate Scalable from bench to production Compatible with hollow fiber and flat sheet TFF Low hold-up volumes |
Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability |
|
| SoloVPE™ Device |
|---|
|
Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability |
Resources
Lentiviral vector manufacturing process enchancement utilizing TFDF™ technology
by Thomas Williams, Oliver Goodyear, Lee Davies, Carol Knevelman, Michael Bransby, Kyriacos Mitrophanous and James Miskin
Cell and Gene Therapy Insights, April 2020