Plasmid DNA is accepted as a starting material for the production of mRNA or viral vectors such as lentivirus vectors and adeno-associated virus (AAV) vectors.
Repligen solutions for plasmid DNA manufacturing
Needs and challenges
- Process improvements are needed, including closed single-use systems, automated and standardized platforms.
- Capacity has to keep up with increasing demand; facility throughput is important.
- Low shear filtration processes are critical as pDNA becomes concentrated.
Repligen solutions can help overcome obstacles in Plasmid DNA bioprocessing.
Click on the unit operation below to find productivity and throughput solutions you can implement today.
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| ProConnex® Flow Paths |
Spectrum® Hollow Fiber Filters |
KrosFlo® TFF Systems |
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Sterile, disposable, sensor-integrated and ready-to-operate Compatible with hollow fiber and flat sheet filters Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk |
Wide range of formats, chemistries and pore sizes Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation |
Fully automated, easy to operate Scalable from bench to production Compatible with hollow fiber and flat sheet TFF Low hold-up volumes |
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|---|---|---|---|---|
| ProConnex® Flow Paths |
Spectrum® Hollow Fiber Filters |
KrosFlo® TFF Systems |
TangenX™ SIUS™ Gamma Flat Sheet Cassettes | SoloVPE™ Analytics |
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Sterile, disposable, sensor-integrated and ready-to-operate Compatible with hollow fiber and flat sheet filters Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk |
Wide range of formats, chemistries and pore sizes Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation |
Fully automated, easy to operate Scalable from bench to production Compatible with hollow fiber and flat sheet TFF Low hold-up volumes |
Closed, ready-to-use, single-use cassettes High flux membranes increase yield, reduce process times, promote consistency, minimize costs Ideal for AAV processes where large volumes need to be concentrated 20-60x prior to chromatography |
Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability |
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| OPUS® Columns |
SoloVPE™ Analytics |
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Ready-to-use, no packing expertise needed Pack any resin for AAV, Lentivirus, or plasmid purification (including custom resins) 0.5 - 30 cm IDs | 5 - 30 cm bed heights |
Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability |
|
|
|
|
|
|---|---|---|---|---|
| ProConnex® Flow Paths |
Spectrum® Hollow Fiber Filters |
KrosFlo® TFF Systems |
TangenX™ SIUS™ Gamma Flat Sheet Cassettes | SoloVPE™ Analytics |
|
Sterile, disposable, sensor-integrated and ready-to-operate Compatible with hollow fiber and flat sheet filters Ideal for Gene Therapy: aseptic processing, reduced cross-contamination risk |
Wide range of formats, chemistries and pore sizes Ideal for UF/DF of large, shear-sensitive molecules, like Lentivirus - high yield and improved impurity removal in process and/or final purified product formulation |
Fully automated, easy to operate Scalable from bench to production Compatible with hollow fiber and flat sheet TFF Low hold-up volumes |
Closed, ready-to-use, single-use cassettes High flux membranes increase yield, reduce process times, promote consistency, minimize costs Ideal for AAV processes where large volumes need to be concentrated 20-60x prior to chromatography |
Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability |
|
| SoloVPE™ Analytics |
|
Rapid and accurate absorbance measurements; Avoid labor-intensive, precision-impacting sample dilution Automated plasmid/viral vector concentration measurement with increased assay variability and repeatability |