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Evolution of upstream intensification: Optimizing perfusion processes with PAT
Process analytical technology (PAT) in bioprocessing has improved manufacturing control while reducing variability and costs in process development and manufacturing. Leveraging PAT with process intensification tools drives robust, efficient, and scalable platforms. In this webinar, Bristol Myers Squibb scientists describe application of PAT to upstream intensification towards developing a mAb N-1 platform process with accelerated timelines. The optimal cell-specific perfusion rate and inoculation density were determined within 2- 3 experiments. The PAT landscape in upstream intensification, the benefits of this approach, and the scale-up from benchtop to manufacturing will be discussed.
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ELISA Kits
ELISA Kits from Repligen deliver the precision, reproducibility, and sensitivity required for the accurate quantitation of leached native Protein A, recombinant Protein A, the MabSelect SuRe™ ligand, the NGL-Impact® ligands, AVIPure ligands, and LONG® R3 IGF-I.
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Development of Manufacturing Therapeutic Platform for EVs Derived from MSC using TFDF and TFF
There has been a growing demand in Extracellular Vesicle (EV) supply in recent years due to their emerging role as intercellular messengers and their therapeutic potential as targeted and natural drug delivery vehicles with high specificity and efficiency
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AVIPure® Albumin Affinity Resin
Discover a high performance affinity chromatography resin for the primary capture of human albumin from complex feedstocks.
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SpectraPor® Biotech Grade Cellulose Ester (CE) Dialysis Membrane
Repligen produces molecular porous Biotech Grade Cellulose Ester (CE) membranes in a large range of MWCO’s specifically for critical MW separations using a proprietary casting process free of heavy metal and sulfur contaminants.  The CE dialysis tubing is ultra-pure and does not require special cleaning or treatment.
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Sustainability
MAKING AN IMPACT At Repligen, sustainability is a strategic priority that impacts all aspects of our organization. Through a balanced approach that blends stakeholder engagement, employee empowerment and strategic investments, we have committed to driving positive impacts across environmental, social and governance topics that are material to our business.
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Viral vector N-1 intensification reduces seed train vessel number, media volume and time
The demand for gene therapy viral vectors requires manufacturing processes to scale up to 2000 L. Viral vector production at this scale requires a 9- to 12-day seed train with two to three seeding bioreactors, increasing the risk of failure and manufacturing cost. Perfusion-based cell culture intensification techniques shorten and simplify the seed train for recombinant proteins and monoclonal antibodies production by reducing time, equipment, bioreactor size and cost while increasing the potential number of batches per year. This webinar will discuss similar efficiency gains for three different viral vectors (lentivirus, AAV8 and AAV9) through intensification using KrosFlo® TFDF® Technology that resulted in reduce train vessel number, N-1 vessel size, media volume and time.
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ProConnex® MixOne CB-X
A cutting-edge single-use mixing system for bioprocess products that boasts exceptional performance for shear-sensitive substances.
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Modalities
​A NEW GENERATION OF BIOLOGICS New modalities, such as viral vectors and mRNA, are critical in addressing challenging targets in bioprocessing. In addition to mAbs and rProteins, these exciting and potent clinical modalities promise faster, more efficient therapeutics with a broad range of potential applications.
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KrosFlo ® Fittings – Repligen
The KrosFlo ® Pro-Connex fittings both simplify and make a Tangential Flow Filtration path more robust. The sanitary flange of the Pro-Connex fitting attaches directly to the Hollow Fiber Filtration Module with a standard sanitary clamp. Tubing and pressure transducers can then be attached to the Pro-Connexs hose barb and female luer port, eliminating the need for tube-to-tube adapters, fittings and connections, Pro-Connex fitting are molded polysulfone, the same low-leachable and highly compatible polymer used in Spectrums hollow fiber module housings. Pro-Connexs are available in 6 sizes, 2 with a 90 degree hose barb fitting which makes connections for some applications easier.