Go with the Flow: CTech FlowVPE System in combination with MALS for Real Time Molecular Weight (RT-MW) Applications
Multi Angle Light Scattering (MALS) can provide the real time molecular weight (Mw) of a pure solute or the average Mw of a solute matrix if the total concentration of the species is also available in real time. Analytical experiments with low protein concentration can use conventional fixed path length UV sensors, however these become saturated at the higher concentrations of manufacturing processes. The FlowVPE System enables the real time measurement of protein content in a wide concentration range, which can be coupled to MALS for RT-MW measurements. Besides a more agile process development, RT-MW can enable adaptive processing decisions and inform on product deviations
Speaker: Andres Martinez-Roche