What is Slope Spectroscopy®?
A Unique and Patented Variable Pathlength Technology (VPT)
Providing a more efficient method for analyzing at-line and in-line processes, slope spectroscopy is a UV-based technology that serves as an alternative to the Beer-Lambert Law that focuses on pathlength as the variable that determines concentration.
How does Slope Spectroscopy work?
Called Slope Spectroscopy due to the slope this technique creates when there is a change in absorbance over a change in pathlength, this method leverages the power and flexibility of variable pathlength technology to create rapid and robust concentration measurements.
UV-based, this method analyzes samples at variable pathlengths using a precision linear stage to efficiently collect multiple absorbance data points to calculate a slope regression. Generating a slope value (absorbance over pathlength) allows you to quantify the concentration with higher accuracy, rather than relying on a single data point using absolute absorbance.
Unlike traditional spectroscopy — where the concentration is the variable — our method uses the pathlength as the variable, allowing the concentration to remain constant. When the concentration remains constant, you are able to eliminate dilution and decrease the chances of contamination and that creates a more reliable end result and product.
Traditional UV
Traditional spectroscopy uses concentration as the variable and a single data point using absolute absorbance
Slope Spectroscopy Method
The Slope Spectroscop Difference
A different approach to UV-Vis
Decrease Errors
Traditional UV-Vis spectroscopy relies on a fixed pathlength at a single absorbance data point. By locking in the pathlength the user is forced to “unlock” or change the concentration to an acceptable level which leads to an error-prone process.
Increase Accuracy
Slope Spectroscopy provides multiple absorbance data points from different pathlengths offering greater insight into sample and measurement results.
Save Time
The Slope Spectroscopy method using our instruments and software can report concentration results in less than two minutes. The PATsmart SoloVPE System works at-line, while the PATsmart FlowVPX System works in-line. Our ViPER software automates your analysis, so you just focus on the sample preparation and the resulting report. The intuitive software detects change in absorbance, and auto adjusts pathlength accordingly.
Reduce Process Steps for Simplicity and Speed
Speak to a specialist today on how to start saving time and resources in your process using Slope Spectroscopy vs. traditional UV-Vis systems.