Videos :: Repligen

Select a video category filter:

Corporate

How It Works

Technologies

Webinars

Applications

Tutorials

Webinars

AAV case study: 3X titer with integrated clarification

Two case studies illustrate multi-fold upstream AAV titer increases for viral vector from HEK293 cells. In addition to productivity gains, XCell™ and TFDF^® technologies clarify conditioned media for manufacturing operational simplicity. Product yield and process economics in the context of the current viral vector supply demand gap will be discussed.

Webinars

Advanced chromatography system design increases yield and reduces deviations

The newly designed Artesyn Chromatography Systems from Repligen execute processes with improved yield, reproducibility, and operational simplicity. Over-molded tubing connections eliminate the use of hose barbs and zip ties, which are prone to leaking. Synergistic multi-valve manifolds create a streamlined liquid path without small crevices that typically cause carry-over between buffers. Batch change-overs can be made in under 30 minutes with no tools. The positive impact on process yield and facility output will be discussed.

How It Works

Welcome to KF Comm 2

KF Comm 2 Software provides a modern, graphically driven interface for process monitoring and control. Create new experiments based on 13 programmed modes, customize batch reports and plug-and-play hardware components. Add an optional 21 CFR Part 11 feature package for regulated environments. Start a run in only 4 clicks Select from 13 pre-programmed modes Operate securely on Microsoft SQL® database for stability

Webinars

Configurability, single-use and compliance for with new automated flat sheet TFF up to 500L

Abstract Driven by the rise in cell and gene therapies, TFF unit operations at scales from 140 mL to 500 L now require the single-use, automation and data integrity capabilities previously reserved for larger scale systems. The newly developed KrosFlo® FS TFF Systems from Repligen provide walk-away automation for multi-step concentration, diafiltration and fed-batch processes with TMP control, closed and single-use flow paths and 21 CFR Part 11 software compliance for processes up to 500 L. Key components to this new system include: a patented automatic back-pressure control valve, a proprietary advanced microprocessor controller that enables plug-and-play hardware connectivity and modern software built upon a secure SQL database. Key technical features and applications supported by flexible configurations of the KrosFlo® FS TFF Systems will be discussed.

Technologies Webinars

Repligen Virtual Seminar | Overcome Bioprocessing Capacity Challenges

Bioprocessing manufacturers are experiencing a surge in demand, and a need for novel solutions that enhance process economics and process optimizations. Led by plenary speaker Prof. J. Christopher Love, topics will include: • Strategic challenges and a vision for on-demand biomanufacturing • Capacity expansion initiatives to meet unprecedented demand • Viral vector manufacturing at scale • Upstream capacity expansion through cell retention based intensification

Webinars

Configurability and Compliance for Automated TFF up to 500L

Driven by the rise in cell and gene therapies, TFF unit operations at scales from 10 mL to 500 L now require the single-use, automation and data integrity capabilities previously reserved for larger scale systems. The newly developed KrosFlo® KMPi TFF System from Repligen provides walk-away automation for concentration, diafiltration and fed-batch processes with TMP control, closed and single-use flow paths and 21 CFR Part 11 software compliance for processes up to 500L. Key components to this new system include: a patented automatic back-pressure control valve, a proprietary control board that enables plug-and-play hardware connectivity and modern software built upon a secure SQL database. Key technical features and applications supported by flexible configurations of the KrosFlo® KMPi TFF System will be discussed.

Webinars

An improved workflow for miniaturized pre-packed columns and automation

A workflow for automated chromatography process development created by Repligen, Tecan, and Synthace enables efficient design, deployment, and analysis of downstream purification strategies. Miniaturized OPUS® RoboColumn® Columns reduce resource demand and allow for parallelization of experiments. In conjunction with robotic liquid handling from Tecan and purpose-built automation software from Synthace, RoboColumn® Column users can optimize yield, purity, and reproducibility for expediting downstream development. The synergistic benefits of OPUS® RoboColumn® Columns, Tecan robotics, and Synthace software will be demonstrated in the context of a case study that would be unapproachable using traditional methods. May 21, 2021 53 minutes

Webinars

XCell™ Lab System for Simplified and Scaled-down Intensification

XCell ATF® technology can expand bioprocess facility capacity and throughput with cell densities up 300 x 106 VCD/mL. The newly launched XCell™ Lab Controller System accelerates development of robust intensified processes at volumes as low as 0.5L, reducing media costs per run and facilitating advancement to manufacturing. The benefits of key features such as a 0.5L single-use device, permeate pressure recording, a pump-based control logic loop, support for two devices per controller and a modern smartphone-like software interface are be discussed.

Webinars

New COVID-19 Affinity Resin Purifies SARS COV-2 Spike Protein in One Step

The new NGL COVID-19 Spike Protein Affinity Resin from Repligen binds the SARS CoV-2 Spike Protein receptor binding domain (RBD) to yield exceptional purity in a single chromatography step. Developed in partnership with Navigo Proteins and Purolite Life Sciences , the product meets all expected requirements of a high-performance resin, including very high selectivity, high dynamic binding capacities and caustic stability over multi-cycle use. Design features of the resin and example performance data will be discussed.