Tube-A-Lyzer® is a ready-to-use device that combines single-use convenience with the efficiency of dynamic dialysis to significantly improve dialysis efficiency and can reduce time from days to hours. The unique two chamber-device features a membrane defined sample chamber with a port for easy sample loading and recovery; surrounded by a flow-through buffer chamber for continuous and gentle dialysis. Compared to traditional-static dialysis, the continuous buffer flow increases the mass transfer rate across the semi-permeable membrane to achieve a higher exchange rate and purity in less time.
The Tube-A-Lyzer® easily mounts on a standard lab stand with a tube clamp and connects to a separate buffer source that is continuously pumped in either single-pass or recirculation mode. Two units can be coupled for operating in parallel. (Stand, pump, pump tubing and bracket sold separately)
Available in 2 sample volume sizes (8-10 ml and 25-30 ml) and made with with high purity Biotech Grade Cellulose Ester (CE) membrane in 6 MWCOs ranging from 0.1-100 kD (refer to the Chemical Compatibility table).
Self-contained cylindrical dynamic dialysis device with concentric sample and buffer chamber
Easy loading and recovery with Luer-Lok Syringe. Continuous buffer flow eliminates buffer changes.
Increases the rate of dialysis and can reduce overall dialysis time from 1-2 days to 6-12 hours
6 MWCOs from 0.1 - 100 kD for all dialysis applications from small peptides to large nanoparticles
Achieve 98% sample purity with Biotech CE membrane, no heavy metal and sulfides.
Purification of fragile proteins
Typical Applications for Tube-A-Lyzer® Dynamic Dialysis Devices
|2 Volume Sizes||8 - 10 ml||25 - 30 ml|
|Buffer chamber volume||50 - 55 ml||120 - 130 ml|
|Total length||23 cm||50 cm|
|Total diameter||2.2 cm||2.2 cm|
|Membrane effective length||14 - 16 mm||36 - 38 mm|
|Membrane diameter||1.0 mm||1.0 mm|
|0.1 - 0.5 kD|
|3.5 - 5 kD|
|Membrane||Biotech grade Cellurose Ester membrane (CE)|
|Sample chamber||Polycarbonate top and bottom piece
Female Luer-lok sample port
Polypropylene male Luer-lok cap
|Buffer chamber||Polycarbonate housing
Polycarbonate end caps
6 mm (3/16 inch) hose-barb inlet / outlet ports
|Dynamic pump rate||10-20 ml/min (should be optimized for application)|
|Packaging||Dry packaged with 20% glycerin|