Ready-to-use dialysis devices are all made with our proprietary Biotech Grade CE dialysis tubing, preassembled with volume specific lengths of membrane tubing and designed to maximize convenience, ease-of-use and sample recovery.
SpectraPor® Tube-A-Lyzer® Dynamic Dialysis Device
Tube-A-Lyzer® is a ready-to-use device that combines single-use convenience with the efficiency of dynamic dialysis to significantly improve dialysis efficiency and can reduce time from days to hours. The unique two chamber-device features a membrane defined sample chamber with a port for easy sample loading and recovery; surrounded by a flow-through buffer chamber for continuous and gentle dialysis. Compared to traditional-static dialysis, the continuous buffer flow increases the mass transfer rate across the semi-permeable membrane to achieve a higher exchange rate and purity in less time.
The Tube-A-Lyzer® easily mounts on a standard lab stand with a tube clamp and connects to a separate buffer source that is continuously pumped in either single-pass or recirculation mode. Two units can be coupled for operating in parallel. (Stand, pump, pump tubing and bracket sold separately)
Available in 2 sample volume sizes (8-10 ml and 25-30 ml) and made with with high purity Biotech Grade Cellulose Ester (CE) membrane in 6 MWCOs ranging from 0.1-100 kD (refer to the Chemical Compatibility table).
Features
- 2 sample volume sizes (8-10 ml & 25-30 ml)
- Easy access port for sample loading, recovery & testing
- Biotech CE membrane in 6 concise MWCO’s (0.1 – 100 kD)
- Flow through buffer chamber for dynamic dialysis
- Coupling for parallel operation or split-flow
UNIQUE DESIGN
Self-contained cylindrical dynamic dialysis device with concentric sample and buffer chamber
EASY TO USE
Easy loading and recovery with Luer-Lok Syringe. Continuous buffer flow eliminates buffer changes.
FASTER
Increases the rate of dialysis and can reduce overall dialysis time from 1-2 days to 6-12 hours
BROAD MWCO RANGE
6 MWCOs from 0.1 - 100 kD for all dialysis applications from small peptides to large nanoparticles
HIGH PURITY
Achieve 98% sample purity with Biotech CE membrane, no heavy metal and sulfides.
FEATURED APPLICATION
Purification of fragile proteins
How it Works
Typical Applications for Tube-A-Lyzer® Dynamic Dialysis Devices
- Gentle purification of labile proteins and conjugates
- Isolation of delicate macromolecules
- Biopolymer purification and removal of monomers
- Buffer exchange for protein refolding
- Enzyme activity and ligand binding studies
Product Specifications
| 2 Volume Sizes | 8 - 10 ml | 25 - 30 ml |
|---|---|---|
| Buffer chamber volume | 50 - 55 ml | 120 - 130 ml |
| Total length | 23 cm | 50 cm |
| Total diameter | 2.2 cm | 2.2 cm |
| Membrane effective length | 14 - 16 mm | 36 - 38 mm |
| Membrane diameter | 1.0 mm | 1.0 mm |
| 6 MWCOs |
|---|
| 0.1 - 0.5 kD |
| 3.5 - 5 kD |
| 8-10 kD |
| 20 kD |
| 50 kD |
| 100 kD |
| Membrane | Biotech grade Cellurose Ester membrane (CE) |
| Sample chamber | Polycarbonate top and bottom piece Polyurethane potting Female Luer-lok sample port Polypropylene male Luer-lok cap |
| Buffer chamber | Polycarbonate housing Polycarbonate end caps 6 mm (3/16 inch) hose-barb inlet / outlet ports |
| Dynamic pump rate | 10-20 ml/min (should be optimized for application) |
| Packaging | Dry packaged with 20% glycerin |
