Introducing SpectraFlo™ Dynamic Dialysis Systems, the only commercially available systems for scalable dynamic dialysis from lab scale to large volume processing
Dynamic Dialysis
Dynamic dialysis is an exciting new technology that utilizes fluid dynamics to increase purification efficiency and improve large buffer handling, ideal for the production of fragile proteins, viscous fluids and polymer gels, such as hyaluronic acid. These closed, high-efficiency systems offer high concentration gradient dialysis without manual buffer changes and in-process sample handling.
SpectraFlo™ DYNAMIC DIALYSIS SYSTEMS
SpectraFlo™ Lab and Process Systems
SpectraFlo™ Mobile Dialysis System
continuous buffer flow
No manual buffer changes
Walkaway convenience
Fully INtegrated
Includes dialysis tank, pump, reservoirs, membrane
Fast implementation time
scale up, scale out
Scale up from lab to process scale.
Scale out from 1 to 6 tanks in series.
dynamic dialysis applications
Dynamic dialysis is ideal for the purification of viscous fluids and hydrogels (hyaluronic acid – HA) that cannot be filtered. Where traditional filtration methods may fail, dynamic dialysis uses flow dynamics to increase both the rate and efficiency of dialysis.
Applications |
Why Dynamic Dialysis is preferred |
Example |
|
Delicate samples |
Gentle separation without shear stress |
Labile proteins and intact viruses |
|
Viscous samples (Hyaluronic Acid) |
Process hydrogels that are too viscous for filtration |
Cross-linked Hyaluronic Acid for aesthetics and orthobiologics |
|
Protein refolding |
Refold proteins to native state using gradual pH |
Overexpressed proteins containing inclusion bodies |
|
High debris lysate |
Will not foul like pressure driven filtration |
Demineralized bone matrix for othopedic surgery |
|
Polymer and Biopolymer synthesis |
Low MWCO enables removal of unbound low |
Oligonucleotides and polypeptides |
|
Scale up of any static dialysis application |
Easy scale up without complex validation or |
Desalting |
Tank Sizes
SpectraFlo™ MODES OF OPERATION
Case Study: model of dynamic dialysis
This model of a GMP batch production of shear-sensitive protein shows an efficent scale-up of large volume dynamic dialysis for purification.
SpectraFlo™ MOBILE FEATURES AND BENEFITS
Features |
Benefits |
|
Up to 6 tanks in series |
Scale up to 12L Sample Size |
|
Parallel or series operation |
Optimize buffer usage |
|
Wet work tray |
Prepare and load samples with secondary containment |
|
Drain manifold |
Load and drain buffer easily |
|
BSE/TSE free polypropylene construction |
Use for GMP processes and cleanroom manufacturing |
|
Collapsible shelves and tubing hooks |
Store complete system in compact space |
Dialysis Membranes
Dialysis membranes from Repligen include biotech-grade, standard-grade and custom membranes that can be used in both lab-scale and process-scale operations.
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Processing Reservoirs
Designed with drain and 4 ports for recirculating or reservoirs in series
Bisectors
Double sample capacity with 2 dialysis tubings in each tank. *Tank not included.
