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KrosFlo® KTF Systems for Tangential Flow Filtration

With sizes ranging up to 5000L, Repligen KrosFlo® KTF Systems for tangential flow filtration (TFF) set the standard for configurable, premium quality systems for modern bioprocessing. 

One integrated system, configurable design

KrosFlo® KTF Pilot and Process Systems overcome many challenges in single-use bioprocessing today. 

Built on the principle of flexibility, one system can be used from small to large scale, using either hollow fiber filters or flat sheet cassettes.  One system can be used upstream or downstream supporting multiple unit operations.

One system
for hollow fiber filters and flat sheet cassettes

One system
from small to large scale operations

One system
for multiple unit operations

 

Configurable, single-use TFF systems for rapid bioprocessing

See how Repligen systems for tangential flow filtration combine unparalleled configurability with premium quality manufacturing. System designs maximize scalability from small to large volumes, flexibility between hollow fiber and flat sheet filter formats, and ability to use the same system in different unit operations while deploying ready-to-use application-specific flow path assemblies. To ensure success, a team of sales specialists and applications experts helps support rapid start-up and robust operation in cGMP processes.

Multiple configurations to suit specific process needs

*Final design configured to customer application

Available configurations

Specification

KrosFlo® KTF Process System

Typical process volumes Up to 5000 Liters
Max. filter area 2 each up to 17.2 m2 (Hollow Fiber)
2 each up to 20 m2 (Cassettes)
Configurable Components
Pump Multiple MagLev pump sizes
Flow rate Pumps 1 and 2, up to 85 LMP
Max. Pressure 2.5 Bar
Min. hold up volume < 0.5 Liter
Tubing ≤ 1" ID
Membranes Hollow fibers and cassettes
Retentate Flow meter
Permeate Flow meter
Clamp-on flow meter Ultrasonic
Clamp-on or flow through Ultrasonic
Feed Scale Tank 4-20 mA or Ethernet
Cabinet width 24" or 30"
Modes of TFF (Client Base) Conc, Diaf, Final Conc, Fed Batch+
Flow Path Sterile ProConnex® Flow Path
TMP control Non-invasive / No Product Contact
Pressure sensors Multiple sizes
Frame Stainless Steel 304, <35Ra
Auxiliary pumps Tri Lobe SS Perisaltic pump
Software and HMI
PLC Allen Bradley
Software IFix 6.0
iHistorian
Dream Reports
E-signature
Delta V Connectivity to Delta V
GMP 21CFR11 Enabled

 

KrosFlo® KTF PILOT AND PROCESS TFF SYSTEMS

Integrated system

Includes filters, ProConnex® Flow Path, pumps, process monitors, software and graphic display on a single skid

Small footprint

configurable

One system with configurable designs to suit multiple user needs, formats, scale and applications

Scalable

1 – 5000L volumes, from process development to commercial manufacturing

custom flow path

Ready-to-operate ProConnex® Flow Path - customized, configurable, complete assemblies​​​​​​​

Global Support

Field Applications and Service Specialists provide worldwide support

QUALITY MANUFACTURING

Membranes, filters and cassettes developed and manufactured in-house at Boston Center of Excellence 

Compatible Hollow Fiber Filter Modules

  Primary
Application
Volume Inlet/ Outlet Side
Port
Scale
Length
Effective Length (cm) Total
Length (cm)
Surface
area

MiniKros

               
MiniKros_2020.png Pilot-scale TFF 5 L - 50 L 1.5" TC 0.75" TC 1 x FL
2 x FL
3 x FL
20
41.5
65
25
46
70
0.16 - 0.31 m2
0.32 - 0.65 m2
0.51 - 1.0 m2

KrosFlo®

               
krosflo_2020.png Production-scale TFF 10 – 100L 3" TC 1.5" TC 1 x FL
2 x FL
3 x FL
20
41.5
65
23
46
69
0.78 - 1.6 m2
1.65 - 3.25 m2
2.55 - 5.10 m2

KrosFlo® MAX

               
krosflo_max_2020.png Production-scale TFF 100L – 1000+L 6" TC 1.5" TC R65
3 x FL
R85
49.9
67.9
107.5
73.5
91.5
131.1
4.30 - 10.40 m2
7.10 - 17.10 m2
11.30 - 11.35 m2

Compatible flat sheet cassettes

0.1 m2
Surface Area

0.5 m2 
Surface Area

1.5 m2 
Surface Area

2.5 m2 
Surface Area

CONFIGURABLE ProConnex® FLOW PATHS

Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration.  Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.

ProConnex® Configurator

In minutes, using an online drag and drop configurator and with customer input, Repligen representatives can configure a ProConnex® Flow Path specific to a process application in real-time.  The output of the ProConnex® Configurator are 3D engineering drawings and a preliminary quote, which greatly expedite the approval, production and delivery of the ProConnex® Flow Path.

Custom flow paths designed in real time in a 3D drag-and-drop environment

Quotes with 3D engineering drawings ready in minutes

repligen global support ensures success


Local Sales Specialists
 

Experienced account managers with deep industry background act as one point of contact for customers

   


Field Application Specialists

Technical experts provide membrane and hardware design optimization, and post-sales support for scale-up

   


Bioprocess Systems Specialists

Seasoned specialists with application, design and automation knowledge supporting large system projects

   


Automation Engineers
 

Software engineers with application knowledge that support system connectivity with other unit operations

   


Field Service Engineers
 

Field-based engineers professionally trained for optimal start-up and post-sales support

 

expert project management

Systems manufacturing center of excellence

KrosFlo® KTF Pilot and Process Systems are developed, engineered, and manufactured at the Repligen Manufacturing Center of Excellence in Boston, Massachusetts.  In this facility, both membranes and systems are produced under the same roof, complemented with R&D expertise on membrane applications. Customers routinely come to the site for demonstrations and training. 

Systems Center of Excellence - Boston, MA

Membrane manufacturing

Systems manufacturing

Training area

 

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

about Repligen Manufacturing

specifications

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*Final design configured to customer application

Configure your krosflo® ktf system

Specification

KrosFlo® KTF Pilot System

Typical process volumes Up to 1000L
Max. filter area 17.2 m2 (Hollow Fiber)
5 m2 (Cassettes)
Configurable Components
Pump MagLev 600 Series
Flow rate Pumps 45 LMP
Max. Pressure 2.0 Bar
Min. hold up volume 2.0 Liter
Tubing 3/4" ID
Membranes Hollow fibers and cassettes
Retentate Flow meter
Permeate Flow meter
Clamp-on flow meter Ultrasonic
Clamp-on or flow through Ultrasonic
Feed Scale Platforms Available and Client 4-20mA
Cabinet width 24"
Modes of TFF (Client Base) Conc, Diaf, Final Conc, Fed Batch+
Flow Path Sterile ProConnex®
TMP control Non-invasive / No Product Contact
Pressure sensor 0.5" and 1.0" Polysulfone Single-Use
Frame Stainless Steel 304, <35Ra
Auxiliary pumps Tri Lobe SS Perisaltic pump
Software and HMI
PLC Allen Bradley
Software IFix 6.0
iHistorian
Dream Reports
E-signature
 
Delta V Connectivity to Delta V
GMP 21CFR11 Enabled

 

*Final design configured to customer application

Configure your krosflo® ktf system

Specification

KrosFlo® KTF Process System

Typical process volumes Up to 5000L
Max. filter area 2 each up t0 17.2 m2 (Hollow Fiber)
2 each up to 20 m2 (Cassettes)
Configurable Components
Pump MagLev 2 each 2000 Series
Flow rate Pumps 1 and 2: 85 LMP
Max. Pressure 2.5 Bar
Min. hold up volume 6 Liter
Tubing 1" ID
Membranes X Series Hollow fibers and cassettes
Retentate Flow meter
Permeate Flow meter
Clamp-on flow meter Ultrasonic
Clamp-on or flow through Ultrasonic
Feed Scale Tank 4-20 mA or Ethernet
Cabinet width 30"
Modes of TFF (Client Base) Conc, Diaf, Final Conc, Fed Batch+
Flow Path Sterile ProConnex®
TMP control Non-invasive / No Product Contact
Pressure sensor 1.0" and 1.38" Sanitary Polysulfone Single-Use
Frame Stainless Steel 304, <35Ra
Auxiliary pumps Tri Lobe SS Perisaltic pump
Software and HMI
PLC Allen Bradley
Software IFix 6.0
iHistorian
Dream Reports
E-signature
 
Delta V Connectivity to Delta V
GMP 21CFR11 Enabled

 

 

KrosFlo® KTF 200

KrosFlo® KTF 600

KrosFlo® KTF 1000

Typical process volumes Up to 200L Up to 1000L Up to 2500L
Max. filter area 1.0m2 (Hollow Fiber)
0.5 m2 (Cassette)
17.2m2 (Hollow Fiber)
5m2 (Cassette)
17.2m2 (Hollow Fiber)
20m2 (Cassette)
Configurable components 
Pump MagLev x 1 (i100) MagLev x 1 (600 Series) MagLev x 1 (2000 Series)
Flow rate Pump 1: 10 LPM Pump 1: 45 LPM Pump 1: 85 LPM
Max. pressure 1.5 Bar 2.0 Bar 2.5 Bar
Min. hold up volume 200 ml 2000 ml 3500 ml
Tubing Connections to Tank 3/8" ID 3/4" ID 1" and 3/4" ID
Hollow Fiber Filter N filter modules N, K, X filter modules X filter modules
Retentate Flow meter Clamp-on flow Ultrasonic

Feed Scale or Client Signal (4-20mA) Platform 220kg Paltform 500kg, 1000kg, 2000kg Platform 2000kg+
Frame Width 24" 24" 24"
Modes of TFF Conc, Diaf, Final Conc, Fed Batch+

Flow-Path Sterile ProConnex® Flow Path

TMP Control Non-invasive value

Single-Use Pressure Sensor Sanitary PS 0.5", 1.0", 1.38"

Frame Stainless Steel 304, < 35Ra

Permeate Pump Tri lobe Peristaltic pump

Software and HMI      
HMI
 
Rockwell 6181P HMI/ Windows 10
PLC Allen Bradley Micrologix 1400

Software IFix 6.0
iHistorian
Dream Reports
E-signature
 







GMP Enables 21CFR11

Delta V Connectivity to Delta V

Permeate Flow Meter Clamp-on or Flow Thru Ultrasonic

 

 

KrosFlo® KTF 650

KrosFlo® KTF 700

KrosFlo® KTF 1600

KrosFlo® KTF 2000

Typical process volumes Up to 1000L Up to 1000L Up to 2500L Up to 5000L
Max. filter area 17.2m2 (Hollow Fiber)
5m2 (Cassette)
17.2m2 (Hollow Fiber)
5m2 (Cassette)
17.2m2 (Hollow Fiber)
20m2 (Cassette)
17.2m2 (Hollow Fiber) x 2
20m2 (Cassette) x 2
Configurable components 
Pump MagLev x 2 MagLev x 2 MagLev x 2 MagLev x 2
Flow rate Pump 1: 4 LPM
Pump 2: 45 LPM
Pump 1: 10 LPM
Pump 2: 45 LPM
Pump 1: 45 LPM
Pump 2: 85 LPM
Pumps 1 and 2: 85 LPM
Max. pressure 1.5 Bar 2.0 Bar 2.5 Bar 2.5 Bar
Min. hold up volume 375 ml 750 ml 4500 ml 6000 ml
Tubing Connections to Tank 1/4" and 3/4" ID 3/8" and 3/4" ID 1" and 3/4" ID 1" ID
Hollow Fiber Filter T,S,N,K,X filter modules S,N,K X filter modules K,X filter modules X filter modules
Retentate Flow meter Clamp-on flow Ultrasonic

Feed Scale or Client Signal
(4-20mA)
Platform 220kg, 500kg, 2000kg Paltform  200kg, 500kg, 2000kg Platform 1000kg+ Platorm 2000kg+
Frame Width 30"
Modes of TFF Conc, Diaf, Final Conc, Fed Batch+

Flow-Path Sterile ProConnex® Flow Path

TMP Control Non-invasive value

Single-Use Pressure Sensor Sanitary PS 0.5", 1.0", 1.38"

Frame Stainless Steel 304, < 35Ra

Permeate Pump Tri lobe Peristaltic pump

Software and HMI
HMI Rockwell 6181P HMI/Windows 10
PLC Allen Bradley Micrologix 1400

Software IFix 6.0
iHistorian
Dream Reports
E-signature
 










GMP Enables 21CFR11

Delta V Connectivity to Delta V

Permeate Flow Meter Clamp-on or Flow Through Ultrasonic

 

 

 

Base Control Platform iFIX 6.0 Software, 300 tags
Batch Reports Dream Reports
Security / Trends E-signature, iHistorian
PLC Allen Bradley PLC Micrologix 1400
Communication Ethernet Communication
  • Direct to iFIX: OPC - DA, UA, AE
  • Ethernet IP - straight to PLC
Interface Options

Remote control via Ethernet IP

Connecting to other SCADA:

  • Delta V, Wonderware, Pi
Regulatory 21CFR11 Enabled - Compliance and Documentation, including Audit Trail
Process Sequence Sequence programmed to Process Requirements
 

 

resources

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