The KrosFlo® KTF TFF Systems were developed for large-scale downstream tangential flow microfiltration or ultrafiltration of shear sensitive cells or process solutions. Both systems use a single-use non-pulsating levitating magnetic pump controlled by a PLC.
KrosFlo® KTF Systems are set up on a mobile cart that supports the hollow fiber filter module, an integrated ProConnex® flow path, an integrated non-invasive retentate control valve, up to two peristaltic pumps, the levitating recirculating pump, a clamp on ultrasonic retentate flow meter, a flow though ultrasonic permeate flow meter and the electrical cabinet with built in 15” color touch-screen HMI. The mobile cart is made of Sanitary 304 Stainless Steel to meet NEMA 4X wash down requirements.
Hollow fiber filter, custom ProConnex® flow path, control valve, peristaltic and recirculating pumps, flow meters, electrical cabinet, 15” color touch-screen interface mounted on a mobile cart
Fully integrated with sterile,custom-engineered ProConnex® flow path
Compatible with KrosFlo® TFF systems from process development to commercial manufacturing
Sequences available in automated, semi-automated or manual operation modes
15" color touch screen computer display
Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.
Unique to Repligen, the KrosFlo® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.
Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration. Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.
KF Comm Software
Microsoft Surface Pro 4 with Excel
Real-time operating parameters
|Effective Length (cm)||Total
|Production-scale TFF||10 – 100L||3" TC||1.5" TC||1 x FL
2 x FL
3 x FL
|0.78 - 1.6 m2
1.65 - 3.25 m2
2.55 - 5.10 m2
|Production-scale TFF||100L – 1000+L||6" TC||1.5" TC||R65
3 x FL
|4.30 - 10.40 m2
7.10 - 17.10 m2
11.30 - 11.35 m2
The KrosFlo® family of TFF Systems for ultrafiltration and diafiltration supports scale up from process development to commercial manufacturing.
KrosFlo® KTF TFF Systems use a single-use non-pulsating levitating magnetic pump controlled by a PLC.