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KrosFlo® KTF TFF Systems

For efficient purification at commercial scale using tangential flow filtration (TFF), the KrosFlo® KTF Systems can process volumes over 2000 liters.
     • 10L - 2000L+ volume

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TFF for commercial production purification

How Hollow Fiber TFF Works

The KrosFlo® KTF TFF Systems were developed for large-scale downstream tangential flow microfiltration or ultrafiltration of shear sensitive cells or process solutions. Both systems use a single-use non-pulsating levitating magnetic pump controlled by a PLC.

KrosFlo® KTF TFF SYSTEMS

KrosFlo® KTF Systems are set up on a mobile cart that supports the hollow fiber filter module,  an integrated ProConnex® flow path, an integrated non-invasive retentate control valve, up to two peristaltic pumps, the levitating recirculating pump, a clamp on ultrasonic retentate flow meter, a flow though ultrasonic permeate flow meter and the electrical cabinet with built in 15” color touch-screen HMI. The mobile cart is made of Sanitary 304 Stainless Steel to meet NEMA 4X wash down requirements.

Integrated system

Hollow fiber filter, custom ProConnex® flow path, control valve, peristaltic and recirculating pumps, flow meters, electrical cabinet, 15” color touch-screen interface mounted on a mobile cart 

custom flow path

Fully integrated with sterile,custom-engineered ProConnex® flow path

Scalable

Compatible with KrosFlo® TFF systems from process development to commercial manufacturing

Flexible

Sequences available in automated, semi-automated or manual operation modes

Easy to use

15" color touch screen computer display 

 

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

about Repligen Manufacturing

Automatic Back Pressure Control Valve

Unique to Repligen, the KrosFlo® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.

  • Automatically adjusts tubing pinch distance according to user-defined set-points for inlet, retentate, permeate or transmembrane pressures (TMP) when attached to the ProConnex® flow path
  • Maintains a uniform TMP throughout the process run, decreasing membrane fouling
  • Controls the permeate pressure and flow during microfiltration applications
  • Releases and tightens the pinch valve as solution viscosity increases or decreases during a process
  • Can be set to start pinching from the open, middle, or closed position
  • Compatible with tubing sizes 13, 14, 16, 25, HP15, 17, HP24, 18, HP35, 36, and HP36

CONFIGURABLE Pro-Connex® FLOW PATHS

Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration.  Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.

ProConnex® Flow Paths

Single Recirculation Pump Systems

Krosflo® KTF 200 System
Krosflo® KTF 600 System
KrosFlo® KTF 1000 System

One levitating recirculating pump up to 10 LPM

One levitating recirculating pump up to 45 LPM

One levitating recirculating pump up to 85 LPM

Up to 1.5 bar operation

Up to 2.0 bar operation

Up to 2.5 bar operation

Minimal recirculation volume of 500 mls with S04 filter flow path with 200 mls in recirculation loop (Total volume 700 mls)

Minimal recirculation volume of 3800 mls with K04 filter flow path with 1000 mls in recirculation loop (Total volume 4800 mls)

Minimal recirculation volume of 6500 mls with X04 filter flow path with 2100 mls in recirculation loop (Total volume 8600 mls)

Sterile TFF ProConnex® Flow Path

Sterile TFF ProConnex® Flow Path

Sterile TFF ProConnex® Flow Path

MF or UF TFF operations

MF or UF TFF operations

MF or UF TFF operations

3/8” ID recirculation tubing connection

¾” ID recirculation tubing connection

1” or ¾” ID recirculation tubing connections

Non-Invassive TMP control valve

Non-Invassive TMP control valve

Non-Invassive TMP control valve

Allen Bradley PLC

Allen Bradley PLC

Allen Bradley PLC

Configurable to Delta V

Configurable to Delta V

Configurable to Delta V

iFix

iFix

iFix

iHistorian

iHistorian

iHistorian

Dream Reports

Dream Reports

Dream Reports

eSignature

eSignature

eSignature

Connectivity to Delta V

Connectivity to Delta V

Connectivity to Delta V

GMP enabled system

GMP enabled system

GMP enabled system

S and N series Spectrum® Hollow Fiber Filters

N, K and X series Spectrum® Hollow Fiber Filters; TangenX™ Flat Sheet Cassettes up to 5.0 m2

X series Spectrum® Hollow Fiber Filters;
TangenX™ Flat Sheet Cassettes up to 15m2

Delivery < 15 weeks

Delivery < 15 weeks

Delivery < 15 weeks

 

Dual Recirculation Pump SYstems

Krosflo® KTF 650 System
Krosflo® KTF 750 System
Krosflo® KTF 1600 System
KrosFlo® KTF 2000 System

Two levitating recirculating pumps

Two levitating recirculating pumps

Two levitating recirculating pumps

Two levitating recirculating pumps

One pump up to 4 LPM;
One pump up to 45 LPM

One pump up to 10 LPM;
One pump up to 45 LPM

One pump up to 45 LPM;
One pump up to 85 LPM

Both pumps up to 85 LPM

Up to 1.5 bar operation

Up to 2.0 bar operation

Up to 2.5 bar operation

Up to 2.5 bar operation

Final recirculation volume down to 300 mls with S02 filter flow path with 100mls in recirculation loop
(Total Volume 400mls)

Final recirculation volume down to 550 mls with S02- filter flow path with 100 mls in recirculation loop
(Total Volume 650mls)

Final recirculation volume down to 4200 mls with N04 filter flow path with 300mls in recirculation loop (Total Volume 4500mls)

Final recirculation volume down to 50 L with two X04 filter flow path with 3 L in recirculation loop
(Total Volume 56 L)

Starting Volume up to 1500L

Starting Volume up to 1500L

Starting Volume up to 2000L

Starting Volume up to 5000L

Sterile TFF Proconnex® Flow Path 
(T, S, and K Modules)

Sterile TFF Proconnex® Flow Path
(S, K and X Modules)

Sterile TFF Proconnex® Flow Path
(N, K and X Modules)

Sterile TFF Proconnex® Flow Path
(X Modules)

MF or UF TFF Operations

MF or UF TFF Operations

MF or UF TFF Operations

MF or UF TFF Operations
(filters run simultaneously)

TangenX™ Cassettes or Spectrum® Hollow Fibers

TangenX™ Cassettes or Spectrum® Hollow Fibers

TangenX™ Cassettes or Spectrum® Hollow Fibers

TangenX™ Cassettes or Spectrum® Hollow Fibers

1/4” and ¾” ID recirculation tubing connection

3/8” and ¾” ID recirculation tubing connection

1.0” and ¾” ID recirculation tubing connection

1” ID recirculation tubing connection

Non-Invassive TMP control valve

Non-Invassive TMP control valve

Non-Invassive TMP control valve

Non-Invassive TMP control valve

Allen Bradley PLC

Allen Bradley PLC

Allen Bradley PLC

Allen Bradley PLC

iFix

iFix

iFix

iFix

iHistorian

iHistorian

iHistorian

iHistorian

Dream Reports

Dream Reports

Dream Reports

Dream Reports

eSignature

eSignature

eSignature

eSignature

Connectivtiy to Delta V

Connectivtiy to Delta V

Connectivtiy to Delta V

Connectivtiy to Delta V

GMP enabled system

GMP enabled system

GMP enabled system

GMP enabled system

Delivery < 15 weeks

Delivery < 15 weeks

Delivery < 15 weeks

Delivery < 15 weeks

 

Compatible Filter Modules

  Primary
Application		 Volume Inlet/ Outlet Side
Port		 Scale
Length		 Effective Length (cm) Total
Length (cm)		 Surface
area

KrosFlo®

                
KrosFlo.png Production-scale TFF 10 – 100L 3" TC 1.5" TC 1 x FL
2 x FL
3 x FL		 20
41.5
65		 23
46
69		 0.78 - 1.6 m2
1.65 - 3.25 m2
2.55 - 5.10 m2

KrosFlo® MAX

                
KrosFlo-MAX.png Production-scale TFF 100L – 1000+L 6" TC 1.5" TC R65
3 x FL
R85		 49.9
67.9
107.5		 73.5
91.5
131.1		 4.30 - 10.40 m2
7.10 - 17.10 m2
11.30 - 11.35 m2

 

See hollow fiber membrane types

Scalable from benchtop to production

The KrosFlo® family of TFF Systems for ultrafiltration and diafiltration supports scale up from process development to commercial manufacturing.

 KrosFlo® KR2i TFF System
1ml - 10L

KrosFlo® KMPi TFF System
10L - 500L

KrosFlo® KTF TFF Systems
500L - 5000L

Components

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KrosFlo® KTF TFF Systems use a single-use non-pulsating levitating magnetic pump controlled by a PLC.

  • KTF 600 Recirculation Pump up to 50LPM @ 30 psig (2 Bar)
  • KTF 650 Recirculation Pumps up to 35ml/min to 50LPM @ 30 psig (2 Bar)
  • KTF 700 Recirculation Pumps up to 350ml/min to 50LPM @ 30 psig (2 Bar)
  • KTF 1000 Recirculation Pump up to 110 LPM @ 43 psig (3 Bar)

 

  • Software: Proficy HMI/SCADA-IFIX
  • 304 stainless steel mobile cart with four locking swivel casters
  • NEMA 4X rated enclosure, CE/UL
  • Pro-Connex® Flow Path stand
  • Retentate control valve that is non-invasive to the process stream
  • KTF 600 Recirculation Pump up to 43 LPM at 30 psig (2 bar)
  • KTF 1000 Recirculation Pump up to 110 LPM @ 43 psig (3 Bar)
  • Ethernet port for connection to central data acquisition system
  • 15” color touchscreen computer and display of reading and the rendering of the flow path
  • Ultrasonic non-invasive retentate flow meter (or flow sensor)
  • Ultrasonic flow through permeate flow meter (or flow sensor)
  • Visual and audible alarms with safety interlocks
  • USB port for data transfer and connection to external mouse or keyboard

Resources

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