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KrosFlo® KTF TFF Systems

For efficient purification at commercial scale using tangential flow filtration (TFF), the KrosFlo® KTF Systems can process volumes over 2000 liters.
     • 10L - 2000L+ volume

TFF for commercial production purification

The KrosFlo® KTF TFF Systems were developed for large-scale downstream tangential flow microfiltration or ultrafiltration of shear sensitive cells or process solutions. Both systems use a single-use non-pulsating levitating magnetic pump controlled by a PLC.

KrosFlo® KTF TFF SYSTEMS

KrosFlo® KTF Systems are set up on a mobile cart that supports the hollow fiber filter module,  an integrated Pro-Connex® flow path, an integrated non-invasive retentate control valve, up to two peristaltic pumps, the levitating recirculating pump, a clamp on ultrasonic retentate flow meter, a flow though ultrasonic permeate flow meter and the electrical cabinet with built in 15” color touch-screen HMI. The mobile cart is made of Sanitary 304 Stainless Steel to meet NEMA 4X wash down requirements.

Integrated system

Hollow fiber filter,  custom Pro-Connex® flow path, control valve, peristaltic and recirculating pumps, flow meters, electrical cabinet, 15” color touch-screen interface mounted on a mobile cart 

custom flow path

Fully integrated with sterile,custom-engineered Pro-Connex® flow path

Scalable

Compatible with KrosFlo® TFF systems from process development to commercial manufacturing

Flexible

Sequences available in automated, semi-automated or manual operation modes

Easy to use

15" color touch screen computer display 

 

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

about Repligen Manufacturing

Automatic Back Pressure Control Valve

Unique to Repligen, the KrosFlo® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.

  • Automatically adjusts tubing pinch distance according to user-defined set-points for inlet, retentate, permeate or transmembrane pressures (TMP) when attached to the Pro-Connex® flow path
  • Maintains a uniform TMP throughout the process run, decreasing membrane fouling
  • Controls the permeate pressure and flow during microfiltration applications
  • Releases and tightens the pinch valve as solution viscosity increases or decreases during a process
  • Can be set to start pinching from the open, middle, or closed position
  • Compatible with tubing sizes 13, 14, 16, 25, HP15, 17, HP24, 18, HP35, 36, and HP36

configurable Pro-Connex® flow paths

Enhanced through an engineering design process, Pro-Connex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration.  Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.

Pro-Connex® Flow Paths

Proprietary KF Comm Software

KF Comm Software

  • Automatically collects process data
  • Provides real-time overviews of the filtration process

Microsoft Surface Pro 4 with Excel

  • Automatically graphs data in Excel-based software
  • 21CFR Part 11 enabled when used with optional KF Comm Compliant Workbook and interface software package from OFNI Systems (ExcelSafe™)

Real-time operating parameters

  • Time
  • Feed Pressure
  • Retentate Pressure
  • Permeate Pressure
  • Feed Flow Rate
  • Diafiltration Flow Rate
  • Permeate Flow Rate 
  • Feed Scale Weight
  • Permeate Scale Weight
  • Pump RPM
  • Notes (Alarms, Process status, Functions)
  • Pump Mode

Processing parameters

  • Transmembrane Pressure (TMP)
  • Pressure Drop (ΔP)
  • Retentate Flow Rate
  • Permeate Flux
  • Volumetric Throughput (VT)
  • Concentration Factor
  • Shear
  • Diafiltration Volumes
  • Process Time
  • UV, Conductivity, and Temperature (with KONDUiT)

Compatible Filter Modules

  Primary
Application
Volume Inlet/ Outlet Side
Port
Scale
Length
Effective Length (cm) Total
Length (cm)
Surface
area

KrosFlo®

               
KrosFlo.png Production-scale TFF 10 – 100L 3" TC 1.5" TC 1 x FL
2 x FL
3 x FL
20
41.5
65
23
46
69
0.78 - 1.6 m2
1.65 - 3.25 m2
2.55 - 5.10 m2

KrosFlo® MAX

               
KrosFlo-MAX.png Production-scale TFF 100L – 1000+L 6" TC 1.5" TC R65
3 x FL
R85
49.9
67.9
107.5
73.5
91.5
131.1
4.30 - 10.40 m2
7.10 - 17.10 m2
11.30 - 11.35 m2

 

See hollow fiber membrane types

Scalable from benchtop to production

The KrosFlo® family of TFF Systems for ultrafiltration and diafiltration supports scale up from process development to commercial manufacturing.

Components

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KrosFlo® KTF TFF Systems use a single-use non-pulsating levitating magnetic pump controlled by a PLC.

  • KTF 600 Recirculation Pump up to 50LPM @ 30 psig (2 Bar)
  • KTF 650 Recirculation Pumps up to 35ml/min to 50LPM @ 30 psig (2 Bar)
  • KTF 700 Recirculation Pumps up to 350ml/min to 50LPM @ 30 psig (2 Bar)
  • KTF 1000 Recirculation Pump up to 110 LPM @ 43 psig (3 Bar)

 

  • Software: Proficy HMI/SCADA-IFIX
  • 304 stainless steel mobile cart with four locking swivel casters
  • NEMA 4X rated enclosure, CE/UL
  • Pro-Connex® Flow Path stand
  • Retentate control valve that is non-invasive to the process stream
  • KTF 600 Recirculation Pump up to 43 LPM at 30 psig (2 bar)
  • KTF 1000 Recirculation Pump up to 110 LPM @ 43 psig (3 Bar)
  • Ethernet port for connection to central data acquisition system
  • 15” color touchscreen computer and display of reading and the rendering of the flow path
  • Ultrasonic non-invasive retentate flow meter (or flow sensor)
  • Ultrasonic flow through permeate flow meter (or flow sensor)
  • Visual and audible alarms with safety interlocks
  • USB port for data transfer and connection to external mouse or keyboard

Resources

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