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Spectrum® Hollow Fiber Membranes

Fully encapsulated in single-use modules, the membranes used in the hollow fiber filter modules from Repligen are constructed using four chemistries that are anisotropic in structure to deliver high flux rates, excellent separation, and low protein binding for higher product yields.

 

Ultrafiltration (UF) membranes have a high density skin layer for less fouling and a fairly open outer support structure for increased filtration rates.

Microfiltration (MF) membranes have a retention defining microporous outer skin and a more open inner layer for increased filtration with higher recoveries.

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

about Repligen Manufacturing

Membrane Types

With a broad choice of single-use module configurations, hollow filter membranes from Repligen use advanced hydrophilic membrane filtration chemistry to provide higher flux rates for faster processing times, excellent selectivity for separation applications, and low protein binding for higher product yields than other membrane chemistries.

hollow fiber module configurations

 

  Membrane Fiber internal diameter MWCO and Pore Size # Module Configurations # Effective Lengths
           
mPES Modified Polyethersulfone 0.50 mm 1kD, 3kD, 5kD, 10kD, 30kD, 50kD, 70kD, 100kD, 300kD, 500kD, 750kD 7 3
mPES Modified Polyethersulfone 0.75 mm 0.65µm 7 3
mPES Modified Polyethersulfone 1.00 mm 3kD, 5kD, 10kD 30kD, 50kD, 70kD, 100kD, 300kD, 500kD, 750kD 7 3
           
ME Mixed Cellulose Ester 0.63 mm 0.1µm, 0.2µm 7 3
ME Mixed Cellulose Ester 1.00 mm 0.2µm 7 3
           
PS Polysulfone  0.5 mm 10kD, 50kD, 500kD and 0.05µm 7 3
           
PES Polyethersulfone 0.5 mm 0.2µm 7 3
PES Polyethersulfone 1.0 mm 0.2µm 7 3

 

Membrane Applications

The following table shows typical use of membrane chemistries by application.

Applications Membrane selection mPES ME PS PES
Concentration and diafiltration (buffer exchange) of proteins, peptides, enzymes, recombinant proteins 1, 3, 5, 10, 30, 50, 70,100kD x   x  
Depyrogenation of water / buffers 3, 10kD x   x  
Coating, diafiltration, fractionation and/or dewatering of nanoparticles 70,100,300,500,750kD, 0.2µm x   x  
Concentration and diafiltration of albumin (nominal MW 67kd) 10kD x      
Concentration and diafiltration of monoclonal antibodies (155kd) 30kD x      
Concentration and diafiltration of envelope and non-envelope virus 50, 70, 100, 300, 500, 750kD, 0.05µm x   x  
Clarification of cell lysates, viral-expressed cultures to recover product in permeate 500, 750kD, 0.05, 0.1µm x x x  
Concentration and washing of insect, bacterial and/or
mammalian cells
750kD, 0.1, 0.2, 0.65µm x x    
Continuous bioreactor perfusion of cell culture 30, 50, 70, 100, 300, 500, 750kD, 0.2, 0.65µm x   x x
Concentration and diafiltration of single-stranded or double-stranded nucleic acids 1, 3, 5, 10, 30, 50, 70,100kD x      
Washing latex, polystyrene and other diagnostic particles to
remove un-reactives / unbound drug substance
500, 750kD, 0.05, 0.1, 0.2, 0.65µm x x x