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XCell ATF® Technology

Simplify and intensify upstream bioprocessing. XCell ATF® intensification solves manufacturing capacity, productivity, and throughput challenges.

NEW | Xcell™ Lab System

The XCell™ Lab system is designed and engineered as a complete solution. The XCell™ Lab system integrates.. NEED INTRO

  • Engineering-designed controller promotes increased intensification
  • Configurable set-up delivers high throughput - a single manifold supports up to 8 XCell ATF® devices
  • Software enables precise technical control with a user-friendly interface
  • New XCell ATF® 1 Device for low volume intensification ready for scale-up
  • Repligen global technical support ensures successful implementation and scale-up

XCell™ Lab System

Fast and easy intensification that scales to manufacturing 


Increase throughput: ​
more programs, ​
more molecules​

Increase productivity: ​
more product per batch​

Increase capacity: ​
more batches per facility


Reduce bioreactor size,
especially < 2000L​

Save > $250 million by solving facility-fit challenges​

Alternative to facility expansion

  • Lower cost​
  • Lower risk​
  • Faster results

Design simplicity:​
software, hardware, device​

Precision pumping enables robust development​

More process data for improved characterization​

Reduced media cost

How and why XCell ATF® Technology works


The XCell ATF® System is based on award-winning alternating tangential flow (ATF) technology, created by the action of a diaphragm moving upward and downward within a pump head, connected to a filter housing and attached to a bioreactor. Alternating tangential flow is attained by the action of a diaphragm pump.

XCell ATF® Devices

XCell™ ATF Single-use System

XCell ATF® - Single-use 

100% cell retention, 80% faster implementation, no autoclave

XCell™ ATF System - Stainless Steel

XCell ATF® - Stainless Steel

100% cell retention, high cell densities and product yields

The XCell ATF® Devices come in multiple sizes from lab-scale to production-scale. Each device consists of a proprietary diaphragm pump and a single-use hollow fiber filter module, for use with an XCell™ Controller.

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system.  We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

about repligen manufacturing

XCell ATF® intensification from Process development to manufacturing​

XCell ATF® applications


Long-term Perfusion

Perfusion, or upstream continuous processing, has been used since the 1980s. High cell density perfusion with XCell ATF® devices delivers higher cell concentration and cell viability.

N-1 Perfusion

N-1 perfusion refers to the intensification of cell growth in the N-1 bioreactor prior to the production N bioreactor, whh remains in fed-batch mode. This process enables high cell density seeding of the production bioreactor.

High Productivity Harvest

High Productivity Harvest (HPH) is a novel application developed by Repligen that enables you to harvest a fed-batch bioreactor in a fully sterile manner while eliminating both centrifugation and depth filtration.

REPLIGEN technical support ENSUres Success

The Repligen Field Application team with expertise in XCell ATF® technology will provide support from consultation to implementation globally. 










Technology fit


Flow path sizing


Experimental plan

Data analysis

System sizing

Long term training plan


About Repligen FAS


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Frequently asked questions | perfusion