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XCell ATF® Technology

XCell ATF® intensification solves upstream manufacturing capacity, productivity, and throughput challenges.

intensification Delivers more Product Faster 

XCell ATF® technology helps simplify and intensify upstream processes to deliver more product, faster. Intensified processes achieve higher cell densities, require smaller bioreactors and consume less suite time. Increase throughput, productivity, and capacity in both clinical and commercial manufacturing.


Increase throughput​
More programs, ​more molecules​

Increase productivity ​
More product per batch​

Increase capacity ​
More batches per facility

Execute high roi ​BUSINESS DECISIONS

Reduce bioreactor size up to 10 fold
For example from 10,000 L to 1,000 L

Advance your program
Smaller, flexible intensified processes

Re-think your facility
Lower cost​, lower risk​, faster results

XCell ATF®, a leading cell retention technology, simplifies and fast-tracks upstream intensification development for gene therapy, fed-batch intensification, seed train intensification, perfusion, and media exchange applications.  

XCell ATF® by application

XCell ATF® intensification from Process development to manufacturing​

The XCell ATF® intensification simplifies and fast-tracks upstream intensification development from PD to commercial scale. Intensification increases throughput, accelerates tech transfer and optimizes your process in clinical manufacturing and MSAT while increasing bioreactor productivity and reducing costs in commercial manufacturing. Higher productivity, smaller bioreactors and higher throughput with shorter times to harvest, combine to mitigate facility expansion risks and capital costs. 

XCell Atf® in Gene Therapy

The XCell ATF® solution delivers an efficiency boost both pre- and post-transfection in the gene therapy workflow. Increase yield and save time during host cell expansion and pre-transfection preparation. Post-transfection options exist for multiple host types. Learn more about Gene therapy solutions at,  Gene Therapy Manufacturing 2.0  


Lentivirus Production

  • Boost output
  • Eliminate centrifugation
  • Reduce depth filtration requirements

AAV Production

  • Boost output
  • Save time

Adenovirus and Vaccine Production

  • Boost output
  • Produce clarified harvest
  • Go straight to downstream

Xcell ATF® Insect Line Delivers 10x VCD and Lowers cogs

Source: ExpreS2ion Technologies

Higher productivity and lower COGs for malaria therapeutic candidate.

  • 10X VCD more than 2-week Fed- Batch (350 x 106 cells/mL) 
  • 50% decrease in bioreactor time
  • 20-fold increase in yield

How XCell ATF® Technology works

The XCell ATF® System is based on award-winning alternating tangential flow (ATF) technology, created by the action of a diaphragm moving upward and downward within a pump head, connected to a filter housing and attached to a bioreactor. Alternating tangential flow is attained by the action of a diaphragm pump.

XCell ATF® Devices and controllers

The XCell ATF® System delivers a complete solution for upstream intensification with robust hardware software integration. Available in single-use and stainless steel formats with sizes from lab-scale (2 L) to process scale (5000 L). Each system consists of an XCell ATF® Device, XCell™ controller, software, tubing and sensors.

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system.  We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

about repligen manufacturing

REPLIGEN technical support ENSUres Success

The Repligen Field Application team with expertise in XCell ATF® technology will provide support from consultation to implementation globally. 


  • Theory
  • Technology fit
  • Experimental plan


  • Demonstration
  • Training
  • Data analysis


  • Optimization
  • Flow Path sizing
  • System sizing


  • Scale-up
  • Validation
  • Long-term training plan


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Frequently asked questions | perfusion