Strategies for dsRNA Removal and Accurate Quantitation in Emerging mRNA Modalities
Immunogenic double-stranded RNA (dsRNA) byproducts are a major impurity complicating the purification of RNA-based vaccines and therapeutics consisting of mRNA, circRNA, or saRNA. In addition, current UV methods of nucleic acid quantitation require extra sample handling in the form of dilutions, which can lead to inaccuracies in measuring mRNA concentration.
In this webinar, Rice University assistant professor Kelsey Swingle, PhD, provides a current overview of mRNA lipid nanoparticle therapeutics and Repligen senior scientist Nathaniel Clark, PhD, discusses emerging purification and quantitation techniques that work for the new generation of mRNA therapeutics beyond COVID-19 vaccines.
Topics include:
- A dsRNA-specific affinity chromatography resin that removes dsRNA byproducts
- How pairing dsRNA affinity chromatography with optimized in vitro transcription (IVT) processes eliminates immunogenicity
- Validation of variable pathlength technology for accurate, reproducible mRNA quantitation
May 12, 2026
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