Designed for low volume, high concentration applications, the KrosFlo® KR2i TFF System from Repligen is designed for robust downstream ultrafiltration and microfiltration during purification and formulation development.
The KrosFlo® KR2i enables faster process development facilitating multiple tests/processes, accurate process definition and execution consistency. Fully integrated and ready to use out of the box, the system includes precise and reproducible control and documentation of TFF processes with volumes from 2mL to 15L, and filtration areas from 13 cm2 to 5000 cm2.
The fully integrated KrosFlo® KR2i TFF System features a digital peristaltic pump, graphical LCD display, digital pressure monitor, KR2i Pump head, automatic backpressure valve, filter module stand, and KF Comm real-time data collection software. Highly configurable and versatile, the KrosFlo® KR2i utilizes a number user-defined set-points to run complex application processes with numerous built-in safety alarms and stops. The system can run up to two stackable pump heads for higher flow rates.
Includes filters, pumps, process monitors, software and graphic display
ProConnex® custom flow paths, 4 filter module configurations, 4 membrane chemistries, 3 effective filter lengths
Compatible with KrosFlo® TFF systems from process development to commercial manufacturing
Use with hollow fiber filters or flat sheet cassettes
Out of the box functionality, short time to system proficiency
KONDUiT add-on module for automated process value indication for conductivity, UV and temperature
Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.
Finally, a Tangential Flow Filtration (TFF) system that monitors concentration during your UF/DF run. No longer are you dependent solely on mass inputs and off-line fixed-pathlength UV-Vis spectrophotometers. Now, you can measure what truly matters in real-time, your concentration.
Unique to Repligen, the KrosFlo® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.
Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration. Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.
KF Comm 2 Software, backward compatible with legacy KrosFlo® KR2i and KrosFlo® KMPi Systems, is conveniently installed on any computer using the Windows Operating System. The software is comptaible with Chrome, Mozilla, Firefox and Microsoft Explorer browsers and is always current with automatic software and firmware updates.
Primary Application |
Volume | Inlet/ Outlet | Side Port |
Scale Length |
Effective Length (cm) | Total Length (cm) |
Surface area |
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MicroKros |
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Process characterization, Small-volume TFF in a laboratory |
1-100 ml | MLL | FLL | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 45 69 |
13 – 20 cm2 26 - 59 cm2 41 - 92 cm2 |
MidiKros |
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Process development at small scale | 100 ml - 3 L | FLL | FLL | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 45 69 |
75 – 190 cm2 155 - 390 cm2 245 - 610 cm2 |
MidiKros TC |
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Process development at pilot scale | 100 ml - 3 L | 1/2" TC | FLL | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 45 69 |
75 – 190 cm2 155 - 390 cm2 245 - 610 cm2 |
MiniKros Sampler |
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Batch TFF of small volumes | 3 L -15 L | 0.75" TC | 0.75" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 47 70 |
490 – 1000 cm2 1000 - 2200 cm2 1600 - 3400 cm2 |
Monitor and evaluate conductivity, UV and temperature for automated process controls. KONDUiT is an add-on module ready to use with the KrosFlo® KR2i and KMPi TFF Systems, with the ability to scale from 2mL to 500L process volumes.
KONDUiT adds:
*UV components sold separately
Conductivity Range | 0.1 to 100 mS/cm |
Temperature Range | 0-70° |
Pressure Range | up to 75 psi (5 bar) |
Dimensions | (L x W x H): 7¾" x 4¾" x 4½" |
Weight | 1.6 kg |
The KrosFlo® family of TFF Systems for concentration and diafiltration supports scale up from process development to commercial manufacturing.
KrosFlo® KR2i TFF System
2ml - 15L
KrosFlo® KMPi TFF System
100mL - 500L
KrosFlo® KTF TFF Systems
10L - 2000L+
Transducers
Scales
Maintain user-defined feed weight set-points, and total permeate mass during TFF processes.
KrosFlo® KR Jr Auxiliary Pump
Auxiliary pumps automatically increase or decrease buffer/product addition to feed reservoir.