KrosFlo^® KR2i TFF System

A proven turnkey benchtop system for lab-scale process development, the KrosFlo^® KR2i Tangential Flow Filtration (TFF) System is fully integrated and easy to use with walkaway automation.
       •  2ml - 15L volume
       •  13 cm² - 5000 cm² filtration area

TFF for lab-scale process Development

 

How hollow fiber TFF works

Designed for low volume, high concentration applications, the KrosFlo^® KR2i TFF System from Repligen is designed for robust downstream ultrafiltration and microfiltration during purification and formulation development.

The KrosFlo^® KR2i enables faster process development facilitating multiple tests/processes, accurate process definition and execution consistency. Fully integrated and ready to use out of the box, the system includes precise and reproducible control and documentation of TFF processes with volumes from 2mL to 15L, and filtration areas from 13 cm² to 5000 cm².

The fully integrated KrosFlo^® KR2i TFF System features a digital peristaltic pump, graphical LCD display, digital pressure monitor, KR2i Pump head, automatic backpressure valve, filter module stand, and KF Comm real-time data collection software. Highly configurable and versatile, the KrosFlo^® KR2i utilizes a number user-defined set-points to run complex application processes with numerous built-in safety alarms and stops. The system can run up to two stackable pump heads for higher flow rates.

Fully integrated

Includes filters, pumps, process monitors, software and graphic display

Configurable

ProConnex^® custom flow paths, 4 filter module configurations, 4 membrane chemistries, 3 effective filter lengths

Scalable

Compatible with KrosFlo^® TFF systems from process development to commercial manufacturing

Flexible 

Use with hollow fiber filters or flat sheet cassettes

Ready to use

Out of the box functionality, short time to system proficiency

KONDUiT walkaway automation

KONDUiT add-on module for automated process value indication for conductivity, UV and temperature

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

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About Repligen manufacturing

Automatic Back pressure Control Valve

Unique to Repligen, the KrosFlo^® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.

• Automatically adjusts tubing pinch distance according to user-defined set-points for inlet, retentate, permeate or transmembrane pressures (TMP) when attached to the ProConnex^® flow path
• Maintains a uniform TMP throughout the process run, decreasing membrane fouling
• Controls the permeate pressure and flow during microfiltration applications
• Releases and tightens the pinch valve as solution viscosity increases or decreases during a process
• Can be set to start pinching from the open, middle, or closed position
• Compatible with tubing sizes 13, 14, 16, 25, HP15, 17, HP24, 18, HP35, 36, and HP36

CONFIGURABLE ProConnex^® FLOW PATH

Enhanced through an engineering design process, ProConnex^® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration.  Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.

ProConnex^® Flow Paths

Proprietary KF Comm Software

KF Comm Software

• Automatically collects process data
• Provides real-time overviews of the filtration process

Microsoft Surface Pro 4 with Excel

• Automatically graphs data in Excel-based software
• 21CFR Part 11 enabled when used with optional KF Comm Compliant Workbook and interface software package from OFNI Systems (ExcelSafe™)

Real-time operating parameters

• Time
• Feed Pressure
• Retentate Pressure
• Permeate Pressure
• Feed Flow Rate
• Diafiltration Flow Rate
• Permeate Flow Rate 
• Feed Scale Weight
• Permeate Scale Weight
• Pump RPM
• Notes (Alarms, Process status, Functions)
• Pump Mode

Processing parameters

• Transmembrane Pressure (TMP)
• Pressure Drop (ΔP)
• Retentate Flow Rate
• Permeate Flux
• Volumetric Throughput (VT)
• Concentration Factor
• Shear
• Diafiltration Volumes
• Process Time
• UV, Conductivity, and Temperature (with KONDUiT)

Compatible hollow fiber Filter Modules

	Primary Application	Volume	Inlet/ Outlet	Side Port	Scale Length	Effective Length (cm)	Total Length (cm)	Surface area
MicroKros
	Process characterization, Small-volume TFF in a laboratory	1-100 ml	MLL	FLL	1 x FL 2 x FL 3 x FL	20 41.5 65	25 45 69	13 – 20 cm2 26 - 59 cm2 41 - 92 cm2
MidiKros
	Process development at small scale	100 ml - 3 L	FLL	FLL	1 x FL 2 x FL 3 x FL	20 41.5 65	25 45 69	75 – 190 cm2 155 - 390 cm2 245 - 610 cm2
MidiKros TC
	Process development at pilot scale	100 ml - 3 L	1/2" TC	FLL	1 x FL 2 x FL 3 x FL	20 41.5 65	25 45 69	75 – 190 cm2 155 - 390 cm2 245 - 610 cm2
MiniKros Sampler
	Batch TFF of small volumes	3 L -15 L	0.75" TC	0.75" TC	1 x FL 2 x FL 3 x FL	20 41.5 65	25 47 70	490 – 1000 cm2 1000 - 2200 cm2 1600 - 3400 cm2

Compatible Flat Sheet Cassettes

TangenX™ SIUS™ PD
Flat Sheet Cassettes

Membrane surface areas

100 cm²
200 cm²
1000 cm²

Monitor and evaluate conductivity, UV and temperature for automated process controls. KONDUiT is an add-on module ready to use with the KrosFlo^® KR2i and KMPi TFF Systems, with the ability to scale from 2mL to 500L process volumes.

KONDUiT adds:

• End point conductivity control for UF /DF
• End point UV control for diafiltering free drug from drug delivery particles
• End point UV control for concentration of certain molecules
• UV alarm for membrane breakthrough
• A260 and A280 light sources available

*UV components sold separately

Scalable from lab-scale to process-scale

The KrosFlo^® family of TFF Systems for concentration and diafiltration supports scale up from process development to commercial manufacturing.