A proven turnkey benchtop system for lab-scale process development, the KrosFlo® KR2i Tangential Flow Filtration (TFF) System is fully integrated and easy to use with walkaway automation.
• 2ml - 15L volume
• 13 cm2 - 5000 cm2 filtration area
TFF for lab-scale process Development
Designed for low volume, high concentration applications, the KrosFlo® KR2i TFF System from Repligen is designed for robust downstream ultrafiltration and microfiltration during purification and formulation development.
The KrosFlo® KR2i enables faster process development facilitating multiple tests/processes, accurate process definition and execution consistency. Fully integrated and ready to use out of the box, the system includes precise and reproducible control and documentation of TFF processes with volumes from 2mL to 15L, and filtration areas from 13 cm2 to 5000 cm2.
KrosFlo® KR2i TFF SYSTEM
The fully integrated KrosFlo® KR2i TFF System features a digital peristaltic pump, graphical LCD display, digital pressure monitor, KR2i Pump head, automatic backpressure valve, filter module stand, and KF Comm real-time data collection software. Highly configurable and versatile, the KrosFlo® KR2i utilizes a number user-defined set-points to run complex application processes with numerous built-in safety alarms and stops. The system can run up to two stackable pump heads for higher flow rates.
Fully integrated
Includes filters, pumps, process monitors, software and graphic display
Configurable
Pro-Connex® custom flow paths, 4 filter module configurations, 4 membrane chemistries, 3 effective filter lengths
Scalable
Compatible with KrosFlo® TFF systems from process development to commercial manufacturing
Flexible
Use with hollow fiber filters or flat sheet cassettes
Ready to use
Out of the box functionality, short time to system proficiency
KONDUiT walkaway automation
KONDUiT add-on module for automated process value indication for conductivity, UV and temperature
Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.
Walkaway TFF Automation
KrosFlo® KR2i with KONDUiT
Perform efficient, automated downstream tangential flow filtration with volumes from 2mL to 10L. Fully integrated and easy to use out of the box, the KrosFlo® KR2i TFF System delivers precise and reproducible control and documentation of tangential flow filtration processes.
Automatic Back pressure Control Valve
Unique to Repligen, the KrosFlo® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.
- Automatically adjusts tubing pinch distance according to user-defined set-points for inlet, retentate, permeate or transmembrane pressures (TMP) when attached to the Pro-Connex® flow path
- Maintains a uniform TMP throughout the process run, decreasing membrane fouling
- Controls the permeate pressure and flow during microfiltration applications
- Releases and tightens the pinch valve as solution viscosity increases or decreases during a process
- Can be set to start pinching from the open, middle, or closed position
- Compatible with tubing sizes 13, 14, 16, 25, HP15, 17, HP24, 18, HP35, 36, and HP36
Configurable Pro-Connex® Flow Path
Enhanced through an engineering design process, Pro-Connex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration. Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.
Proprietary KF Comm Software
KF Comm Software
- Automatically collects process data
- Provides real-time overviews of the filtration process
Microsoft Surface Pro 4 with Excel
- Automatically graphs data in Excel-based software
- 21CFR Part 11 enabled when used with optional KF Comm Compliant Workbook and interface software package from OFNI Systems (ExcelSafe™)
Real-time operating parameters
- Time
- Feed Pressure
- Retentate Pressure
- Permeate Pressure
- Feed Flow Rate
- Diafiltration Flow Rate
- Permeate Flow Rate
- Feed Scale Weight
- Permeate Scale Weight
- Pump RPM
- Notes (Alarms, Process status, Functions)
- Pump Mode
Processing parameters
- Transmembrane Pressure (TMP)
- Pressure Drop (ΔP)
- Retentate Flow Rate
- Permeate Flux
- Volumetric Throughput (VT)
- Concentration Factor
- Shear
- Diafiltration Volumes
- Process Time
- UV, Conductivity, and Temperature (with KONDUiT)
| Process Control Modes | Primary Application | |
|---|---|---|
| M | Manual Mode | Manual operation of pumps and auxiliary components |
| CONT | Continuous Mode | Manual operation of pumps and auxiliary components with operator interface step of operation (concentration and diafiltration) |
| C | Concentration Mode |
Simple concentration of the process solution |
| C/D | Concentration / Diafiltration | Concentration and then diafiltration of the process solution in succession |
| C/D/C | Concentration / Diafiltration / Concentration | Diafiltration of the process solution with one buffer before concentration of the process solution a second time |
| C/D/D/C | Concentration / Diafiltration / Diafiltration / Concentration | Diafiltration of the process solution with two different buffers in succession before concentration of the process solution a second time |
| CFC | Constant Feed Concentration | Concentration of the solution while maintaining a constant feed weight |
| CF/D/C | Constant Feed Concentration / Diafiltration / Concentration | Concentration of the solution while maintaining a constant feed weight, then washing the solution with a buffer during diafiltration, followed by further concentration of the solution |
Compatible hollow fiber Filter Modules
| Primary Application |
Volume | Inlet/ Outlet | Side Port |
Scale Length |
Effective Length (cm) | Total Length (cm) |
Surface area |
|
|---|---|---|---|---|---|---|---|---|
MicroKros® |
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 |
Process characterization, Small-volume TFF in a laboratory |
1-100 ml | MLL | FLL | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 45 69 |
13 – 20 cm2 26 - 59 cm2 41 - 92 cm2 |
MidiKros |
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 |
Process development at small scale | 100 ml - 3 L | FLL | FLL | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 45 69 |
75 – 190 cm2 155 - 390 cm2 245 - 610 cm2 |
MidiKros TC |
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 |
Process development at pilot scale | 100 ml - 3 L | 1/2" TC | FLL | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 45 69 |
75 – 190 cm2 155 - 390 cm2 245 - 610 cm2 |
MiniKros Sampler |
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 |
Batch TFF of small volumes | 3 L -15 L | 0.75" TC | 0.75" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 47 70 |
490 – 1000 cm2 1000 - 2200 cm2 1600 - 3400 cm2 |
see hollow fiber membrane types
Compatible Flat Sheet Cassettes
KONDUiT Add-on Monitor
Monitor and evaluate conductivity, UV and temperature for automated process controls. KONDUiT is an add-on module ready to use with the KrosFlo® KR2i and KMPi TFF Systems, with the ability to scale from 2mL to 500L process volumes.
KONDUiT adds:
- End point conductivity control for UF /DF
- End point UV control for diafiltering free drug from drug delivery particles
- End point UV control for concentration of certain molecules
- UV alarm for membrane breakthrough
- A260 and A280 light sources available
*UV components sold separately
| Conductivity Range | 0.1 to 100 mS/cm |
| Temperature Range | 0-70° |
| Pressure Range | up to 75 psi (5 bar) |
| Dimensions | (L x W x H): 7¾" x 4¾" x 4½" |
| Weight | 1.6 kg |
Scalable from lab-scale to process-scale
The KrosFlo® family of TFF Systems for concentration and diafiltration supports scale up from process development to commercial manufacturing.
KrosFlo® KR2i TFF System
2ml - 15L
KrosFlo® KMPi TFF System
100mL - 500L
KrosFlo® KTF TFF Systems
10L - 2000L+
Systems, Components and accessories
- IP 33 Enclosure
- 600 RPM pump with reversible drive
- 90-260 Vrms @ 50/60 Hz
- Standard Easy Load 3 Pump Head for precision tubing L/S number 13, 14, 16, 25, 17, and 18 provided.
- (Optional) Easy Load 3 pump head for high performance tubing L/S numbers 15, 24, 35, 36
- ETL listed and CE/UL/cUL marked
- Pump Drive Dimensions: 10.5 in x 8 in x 8 in
Transducers
- Measures and displays 4 pressures: Feed Pressure, Retentate Pressure, Permeate Pressure, Transmembrane Pressure (TMP)
- User-defined pressure alarms and stop set-points
- Available with different connector types (Luer and TC) and selected materials of construction (PS, PC)
Scales
Maintain user-defined feed weight set-points, and total permeate mass during TFF processes.
Monitor
User-defined pressure alarms help ensure maximum sample recovery and membrane integrity.
KrosFlo® KR Jr Auxiliary Pump
Auxiliary pumps automatically increase or decrease buffer/product addition to feed reservoir.
TangenX™ SIUS™ Flat Sheet Cassette Clamps
Stainless steel plates designed to be used with TangenX™ SIUS™ and SIUS™ PD Cassettes and filter plate inserts.
