When used for cell culture clarification, TFDF® Technology delivers unprecedented high flux, high capacity, low turbidity, and minimal dilution. The result is a single-use, Gamma-irradiated and closed solution with linear scaling from 1 L to 2000 L. Clarification with the TFDF® Technology provides an alternative to centrifugation and primary depth filters. TFDF® output turbidity and impurity profile are highly comparable to those of centrifugation.
Tangential Flow
High cell density
Poor product passage
Depth Filtration
Low cell density
Excellent product passage
Tangential Flow Depth Filtration
High cell density
Excellent product passage
Tangential flow through the lumen
Thick wall depth filter
Synergistic Benefits
High flux single-use filtration for Fed-Batch clarification
Combines tangential flow with depth filtration
High flux, high capacity, low turbidity, minimal dilution
Integrated hardware, software, flow path
Automated process control logic
Systems scale from lab to process
Flow paths capacity increases with tube number and length
Work with Repligen Field Applications Specialists from consultation to implementation
Cell Culture Feed
Volume 1.0 L
%PCV 6.6 %
VCC 2.5 x 107
Viability 80%
Titer 3.0 g/L
Turbidity 1196 NTU
Permeate Pool
Pool Volume 1.2 L
Process Time 6 hours
Yield > 90%
Turbidity 88 NTU
NTU reduction 92%
Cell Culture Feed
Volume 1.0 L
%PCV 6.6 %
VCC 2.5 x 107
Viability 80%
Turbidity 1182 NTU
Permeate Pool
Pool Volume 1.2 L
Process Time 3 hours
Yield > 90%
Turbidity 94 NTU
NTU reduction 91%
Cell Culture Feed
Volume 1.0 L
%PCV 6.6 %
VCC 2.5 x 107
Viability 80%
Titer 3.0 g/L
Turbidity 1182 NTU
Permeate Pool
Pool Volume 1.2 L
Process Time 1.5 hours
Yield > 90%
Titer 3.0 g/L
Turbidity 101 NTU
NTU reduction 91%
Cell Culture Feed
Volume 500L
%PCV 7%
VCC 1.5 x 107
Viability 80%
Titer 3.0 g/L
Comparable or lower HCP and DNA levels to centrifugation and depth filtration
No filter fouling with high LMH
Volume 1L
%PCV 6.8%
VCC 2.3 x 107
Viability 92%
Titer 5.9 g/L
CC Feed Turbidity 954 NTU
Permeate Pool Turbidity 92 NTU
Flux 1300 LMH
The Repligen Field Application team with expertise in TFDF® technology will provide support from consultation to implementation globally.
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Evaluation |
Development |
Implementation |
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Lentiviral vector manufacturing process enchancement utilizing TFDF® technology
by Thomas Williams, Oliver Goodyear, Lee Davies, Carol Knevelman, Michael Bransby, Kyriacos Mitrophanous and James Miskin
Cell and Gene Therapy Insights, April 2020
