XCell ATF® Systems simplify and fast-track upstream intensification by providing an integrated solution consisting of an ATF Device, XCell™ controller, software, tubing, pressure sensors and accessories. The family of Systems supports diverse applications across development from 1 L to 5000 L.
Xcell ATF® Device Size selection guide
Presented are hollow fiber filter areas, XCell ATF® pump volumes, and typical bioreactor working volumes paired with each XCell ATF® Device. Actual capacity and vessel size depend upon specific cell culture process conditions.
| Device | Effective filter surface area (m2) |
Approximate ATF volume exchanged (L) |
Suspension cultures volume (L) |
|---|---|---|---|
| XCell ATF® 1 | 0.022 | 0.017 | 0.5 - 2 |
| XCell ATF® 2 | 0.13 | 0.1 | 2 - 10 |
| XCell ATF® 4 | 0.77 | 0.4 | 10 - 50 |
| XCell ATF® 6 | 2.5 | 1.3 | 50 - 200 |
| XCell ATF® 10 | 11.0 | 6.0 | 200 - 1000 |
Xcell ATF® Devices by Scale
XCell ATF® Devices By Format
XCell ATF® 2 Single-use Device
XCell ATF® 6 Single-use Device
XCell ATF® 10 Single-use Device
XCell ATF® Single-use Tubing Sets
XCell ATF® 6 Single-use Tubing Set
XCell ATF® 10 Single-use Tubing Set
Filter Wetting Procedure
XCell ATF® single-use devices are supplied dry. To ensure robust filter performance, it is important to wet the filter prior to use. The XCell ATF® Tubing Set and the suggested wetting protocols are designed to:
- Ensure efficient and complete filter wetting by minimizing the formation of air pockets during the wetting process
- Enable pre-use filter integrity testing while maintaining sterility
- Provide a means for evaluating the sterility of the XCell ATF® device prior to establishing the bioreactor connection
- Allow change-out of the XCell ATF® device during a cell culture run without interrupting the bioreactor or XCell ATF® operation
Two wetting procedures have been developed: an off-line procedure and an on-line procedure. After filter wetting, a filter integrity test can be performed. Additional detail can be obtained in the XCell ATF® Single-use User Guides.
Watch the video to see the XCell ATF® offline wetting procedure.
XCell ATF® 2 Stainless Steel Device
XCell ATF® 4 Stainless Steel Device
XCell ATF® 6 Stainless Steel Device
XCell ATF® 10 Stainless Steel Device
How to Install a Hollow Fiber Filter
Watch the video on how to install a hollow fiber filter into an XCell ATF® 10 stainless steel device.
for use with:
XCell ATF® 1 (SU)
XCell ATF® 2 (SU,SS)
XCell® ATF 4 (SS)
XCell ATF® C410 Controller
for use with:
XCell ATF® 4 (SS)
XCell ATF® 6 (SU, SS)
XCell ATF® 10 (SU, SS)
XCell ATF® C410 Controller Cart
(optional)
