XCell ATF Devices and Controllers

XCell ATF^® Systems simplify and fast-track upstream intensification by providing an integrated solution consisting of an ATF Device, XCell™ controller, software, tubing, pressure sensors and accessories. The family of Systems supports diverse applications across development from 1 L to 5000 L.

Xcell ATF^® Device Size selection guide

Presented are hollow fiber filter areas, XCell ATF^® pump volumes, and typical bioreactor working volumes paired with each XCell ATF^® Device. Actual capacity and vessel size depend upon specific cell culture process conditions.


Device	Effective filter surface area (m²)	Approximate ATF volume exchanged (L)	Suspension cultures volume (L)
XCell ATF^® 2	0.13	0.1	1 - 10
XCell ATF^® 4	0.77	0.4	7 - 25
XCell ATF^® 6	2.5	1.2	20 - 200
XCell ATF^® 10	11.0	6.0	200 - 1000

Xcell ATF^® Devices by Scale

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Lab Scale

Pilot Scale

Process Scale

XCell ATF^® Devices By Format

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XCell ATF^® Single-use Devices

XCell ATF^® 2 Single-use Device

XCell ATF^® 6 Single-use Device

XCell ATF^®10 Single-use Device

XCell ATF® Single-use Tubing Sets

XCell ATF^® 6 Single-use Tubing Set

XCell ATF^® 10 Single-use Tubing Set

Filter Wetting Procedure

XCell ATF^® single-use devices are supplied dry. To ensure robust filter performance, it is important to wet the filter prior to use. The XCell ATF^® Tubing Set and the suggested wetting protocols are designed to:

Ensure efficient and complete filter wetting by minimizing the formation of air pockets during the wetting process
Enable pre-use filter integrity testing while maintaining sterility
Provide a means for evaluating the sterility of the XCell ATF^® device prior to establishing the bioreactor connection
Allow change-out of the XCell ATF^® device during a cell culture run without interrupting the bioreactor or XCell ATF^® operation

Two wetting procedures have been developed: an off-line procedure and an on-line procedure. After filter wetting, a filter integrity test can be performed. Additional detail can be obtained in the XCell ATF^® Single-use User Guides.

Watch the video to see the XCell ATF^® offline wetting procedure.