Presented are hollow fiber filter areas, XCell ATF® pump volumes, and typical bioreactor working volumes paired with each XCell ATF® Device. Actual capacity and vessel size depend upon specific cell culture process conditions.
Device | Effective filter surface area (m2) |
Approximate ATF volume exchanged (L) |
Suspension cultures volume (L) |
---|---|---|---|
XCell ATF® 2 | 0.13 | 0.1 | 1 - 10 |
XCell ATF® 4 | 0.77 | 0.4 | 7 - 25 |
XCell ATF® 6 | 2.5 | 1.2 | 20 - 200 |
XCell ATF® 10 | 11.0 | 6.0 | 200 - 1000 |
XCell ATF® 2 Single-use Device
XCell ATF® 6 Single-use Device
XCell ATF® 10 Single-use Device
XCell ATF® Single-use Tubing Sets
XCell ATF® 6 Single-use Tubing Set
XCell ATF® 10 Single-use Tubing Set
Filter Wetting Procedure
XCell ATF® single-use devices are supplied dry. To ensure robust filter performance, it is important to wet the filter prior to use. The XCell ATF® Tubing Set and the suggested wetting protocols are designed to:
Two wetting procedures have been developed: an off-line procedure and an on-line procedure. After filter wetting, a filter integrity test can be performed. Additional detail can be obtained in the XCell ATF® Single-use User Guides.
Watch the video to see the XCell ATF® offline wetting procedure.
XCell ATF® 2 Stainless Steel Device
XCell ATF® 4 Stainless Steel Device
XCell ATF® 6 Stainless Steel Device
XCell ATF® 10 Stainless Steel Device
How to Install a Hollow Fiber Filter
Watch the video on how to install a hollow fiber filter into an XCell ATF® 10 stainless steel device.
XCell ATF® C24 Controller
for use with:
XCell ATF® 2 (SU, SS)
XCell ATF® 4 (SS)
XCell ATF® C410 Controller
for use with:
XCell ATF® 4 (SS)
XCell ATF® 6 (SU, SS)
XCell ATF® 10 (SU, SS)
XCell ATF® C410 Controller Cart
(optional)
Demonstration of XCell ATF® 2 and XCell ATF® 6 Single-use Performance
Mitigation of Protein Retention in Perfusion Process and Effect of Filter Pore Size